Restriction map for
pFN21AE2486
(Linear) (Six-base) MAPSORT of: KIEE2486.seq Check: 704 from: 1 to: 6669
KIEE2486.orf
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
AatII G_ACGT'C
Cuts at: 0 899 3314 3407 6669
Size: 899 2415 93 3262
Fragments arranged by size:
3262 2415 899 93
AccI GT'mk_AC
Cuts at: 0 899 3449 6669
Size: 899 2550 3220
Fragments arranged by size:
3220 2550 899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 2086 2310 3895 4349 4627 4807 6207
Size: 490 1596 224 1585 454 278 180 1400
Cuts at: 6207 6669
Size: 462
Fragments arranged by size:
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180
AfeI AGC'GCT
Cuts at: 0 1252 2893 6669
Size: 1252 1641 3776
Fragments arranged by size:
3776 1641 1252
AflIII A'CryG_T
Cuts at: 0 4271 6041 6669
Size: 4271 1770 628
Fragments arranged by size:
4271 1770 628
AhdI GACnn_n'nnGTC
Cuts at: 0 321 1475 5469 6669
Size: 321 1154 3994 1200
Fragments arranged by size:
3994 1200 1154 321
AleI CACnn'nnGTG
Cuts at: 0 1004 1612 4834 6669
Size: 1004 608 3222 1835
Fragments arranged by size:
3222 1835 1004 608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 1550 2569 3943 6669
Size: 358 1192 1019 1374 2726
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2398 2430 6669
Size: 2398 32 4239
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 49 1377 1398 2147 3716 4107 4398 5908
Size: 49 1328 21 749 1569 391 291 1510
Cuts at: 5908 6669
Size: 761
Fragments arranged by size:
1569 1510 1328 761 749 391 291 49
21
ApaI G_GGCC'C
Cuts at: 0 1405 1436 1782 3131 4821 5278 6598 6669
Size: 1405 31 346 1349 1690 457 1320 71
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 4449 6669
Size: 4449 2220
AvaI C'yCGr_G
Cuts at: 0 436 1557 1692 1713 1728 3044 3233 3265
Size: 436 1121 135 21 15 1316 189 32
Cuts at: 3265 3463 4778 4906 5248 5283 5304 5620 6094
Size: 198 1315 128 342 35 21 316 474
Cuts at: 6094 6164 6282 6669
Size: 70 118 387
Fragments arranged by size:
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198 189 135 128 118 70 35 32
21 21 15
AvrII C'CTAG_G
Cuts at: 0 2769 6669
Size: 2769 3900
BamHI G'GATC_C
Cuts at: 0 587 5748 6669
Size: 587 5161 921
Fragments arranged by size:
5161 921 587
BanI G'GyrC_C
Cuts at: 0 162 917 1006 1388 2246 2391 2735 2765
Size: 162 755 89 382 858 145 344 30
Cuts at: 2765 3040 3210 3223 3864 4364 5308 6573 6603
Size: 275 170 13 641 500 944 1265 30
Cuts at: 6603 6669
Size: 66
Fragments arranged by size:
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275 170 162 145 89 66 30 30
13
BanII G_rGCy'C
Cuts at: 0 91 1156 1405 1436 1451 1729 1735 1744
Size: 91 1065 249 31 15 278 6 9
Cuts at: 1744 1782 2087 2644 2845 2917 3131 4821 4919
Size: 38 305 557 201 72 214 1690 98
Cuts at: 4919 5249 5278 5479 5687 5977 6274 6532 6598
Size: 330 29 201 208 290 297 258 66
Cuts at: 6598 6669
Size: 71
Fragments arranged by size:
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258 249 214 208 201 201 98 91
72 71 66 38 31 29 15 9
6
BbeI G_GCGC'C
Cuts at: 0 166 921 1392 2250 3044 3227 3868 4368
Size: 166 755 471 858 794 183 641 500
Cuts at: 4368 5312 6607 6669
Size: 944 1295 62
Fragments arranged by size:
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183 166 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 648 1474 2025 2275 3306 3443 3735 6669
Size: 648 826 551 250 1031 137 292 2934
Fragments arranged by size:
2934 1031 826 648 551 292 250 137
BbvCI CC'TCA_GC
Cuts at: 0 3780 6379 6669
Size: 3780 2599 290
Fragments arranged by size:
3780 2599 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1956 1990 3381 3415 3540 3574 4702 4736
Size: 1956 34 1391 34 125 34 1128 34
Cuts at: 4736 5221 5255 6669
Size: 485 34 1414
Fragments arranged by size:
1956 1414 1391 1128 485 125 34 34
34 34 34
BclI T'GATC_A
Cuts at: 0 1058 1166 1551 6669
Size: 1058 108 385 5118
Fragments arranged by size:
5118 1058 385 108
BfrBI ATG'CAT
Cuts at: 0 2343 6669
Size: 2343 4326
BglI GCCn_nnn'nGGC
Cuts at: 0 983 1636 1724 3247 5645 5921 6209 6335
Size: 983 653 88 1523 2398 276 288 126
Cuts at: 6335 6508 6669
Size: 173 161
Fragments arranged by size:
2398 1523 983 653 288 276 173 161
126 88
BglII A'GATC_T
Cuts at: 0 1310 2018 3797 6669
Size: 1310 708 1779 2872
Fragments arranged by size:
2872 1779 1310 708
BlpI GC'TnA_GC
Cuts at: 0 50 6669
Size: 50 6619
Bme1580I G_kGCm'C
Cuts at: 0 578 1405 1436 1576 1782 2396 2738 2768
Size: 578 827 31 140 206 614 342 30
Cuts at: 2768 3131 3215 4453 4821 5278 5938 6135 6576
Size: 363 84 1238 368 457 660 197 441
Cuts at: 6576 6598 6669
Size: 22 71
Fragments arranged by size:
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363 342 206 197 140 84 71 31
30 22
BmgBI CAC'GTC
Cuts at: 0 4538 6044 6669
Size: 4538 1506 625
Fragments arranged by size:
4538 1506 625
BmrI ACTGGGnnnn_n'
Cuts at: 0 572 1191 1822 2741 2767 6669
Size: 572 619 631 919 26 3902
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 3994 5583 6669
Size: 30 3964 1589 1086
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 742 1248 1258 2035 2074 2402 2656
Size: 133 609 506 10 777 39 328 254
Cuts at: 2656 2949 3264 3586 4390 4537 4564 4615 4723
Size: 293 315 322 804 147 27 51 108
Cuts at: 4723 5275 5844 6140 6655 6669
Size: 552 569 296 515 14
Fragments arranged by size:
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322 315 296 293 254 147 133 108
51 39 27 14 10
Bpu10I CC'TnA_GC
Cuts at: 0 3780 5095 5681 6112 6199 6379 6669
Size: 3780 1315 586 431 87 180 290
Fragments arranged by size:
3780 1315 586 431 290 180 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 841 2965 3892 4540 6669
Size: 379 462 2124 927 648 2129
Fragments arranged by size:
2129 2124 927 648 462 379
BsaI GGTCTCn'nnnn_
Cuts at: 0 1201 5025 5693 6367 6669
Size: 1201 3824 668 674 302
Fragments arranged by size:
3824 1201 674 668 302
BsaAI yAC'GTr
Cuts at: 0 1095 6669
Size: 1095 5574
BsaBI GATnn'nnATC
Cuts at: 0 1045 1945 4714 6669
Size: 1045 900 2769 1955
Fragments arranged by size:
2769 1955 1045 900
BsaHI Gr'CG_yC
Cuts at: 0 163 896 918 1389 1600 2247 2609 3041
Size: 163 733 22 471 211 647 362 432
Cuts at: 3041 3224 3311 3404 3865 4365 5228 5309 6053
Size: 183 87 93 461 500 863 81 744
Cuts at: 6053 6604 6647 6669
Size: 551 43 22
Fragments arranged by size:
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432 362 211 183 163 93 87 81
43 22 22
BsaWI w'CCGG_w
Cuts at: 0 5798 6669
Size: 5798 871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1072 1102 1246 1276 1786 1816 2375 2398
Size: 1072 30 144 30 510 30 559 23
Cuts at: 2398 2405 2428 2878 2908 2995 3025 4219 4249
Size: 7 23 450 30 87 30 1194 30
Cuts at: 4249 5386 5416 5479 5509 6286 6316 6669
Size: 1137 30 63 30 777 30 353
Fragments arranged by size:
1194 1137 1072 777 559 510 450 353
144 87 63 30 30 30 30 30
30 30 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 130 739 793 1321 1794 1837 1840
Size: 15 115 609 54 528 473 43 3
Cuts at: 1840 1843 1846 1849 2011 3370 3891 4363 4720
Size: 3 3 3 162 1359 521 472 357
Cuts at: 4720 4861 5487 5791 6007 6337 6669
Size: 141 626 304 216 330 332
Fragments arranged by size:
1359 626 609 528 521 473 472 357
332 330 304 216 162 141 115 54
43 15 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 42 624 1073 2779 3295 3675 4064 4391
Size: 42 582 449 1706 516 380 389 327
Cuts at: 4391 5601 6061 6669
Size: 1210 460 608
Fragments arranged by size:
1706 1210 608 582 516 460 449 389
380 327 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1636 3079 3994 5055 5563 6273 6669
Size: 703 933 1443 915 1061 508 710 396
Fragments arranged by size:
1443 1061 933 915 710 703 508 396
BsiEI CG_ry'CG
Cuts at: 0 330 1683 3333 5738 6213 6489 6570 6669
Size: 330 1353 1650 2405 475 276 81 99
Fragments arranged by size:
2405 1650 1353 475 330 276 99 81
BsiHKAI G_wGCw'C
Cuts at: 0 395 1226 1744 1982 2264 2558 2644 2917
Size: 395 831 518 238 282 294 86 273
Cuts at: 2917 3932 4268 4453 4523 4919 5144 5162 5186
Size: 1015 336 185 70 396 225 18 24
Cuts at: 5186 5249 6368 6669
Size: 63 1119 301
Fragments arranged by size:
1119 1015 831 518 396 395 336 301
294 282 273 238 225 185 86 70
63 24 18
BsiWI C'GTAC_G
Cuts at: 0 979 6669
Size: 979 5690
BsmI GAATG_Cn'
Cuts at: 0 4817 5716 6669
Size: 4817 899 953
Fragments arranged by size:
4817 953 899
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1876 5421 5548 5745 6638 6669
Size: 1876 3545 127 197 893 31
Fragments arranged by size:
3545 1876 893 197 127 31
Bsp1286I G_dGCh'C
Cuts at: 0 91 395 578 1156 1226 1405 1436 1451
Size: 91 304 183 578 70 179 31 15
Cuts at: 1451 1576 1729 1735 1744 1782 1982 2087 2264
Size: 125 153 6 9 38 200 105 177
Cuts at: 2264 2396 2558 2644 2738 2768 2845 2917 3131
Size: 132 162 86 94 30 77 72 214
Cuts at: 3131 3215 3932 4268 4453 4523 4821 4919 5144
Size: 84 717 336 185 70 298 98 225
Cuts at: 5144 5162 5186 5249 5278 5479 5687 5938 5977
Size: 18 24 63 29 201 208 251 39
Cuts at: 5977 6135 6274 6368 6532 6576 6598 6669
Size: 158 139 94 164 44 22 71
Fragments arranged by size:
717 578 336 304 298 251 225 214
208 201 200 185 183 179 177 164
162 158 153 139 132 125 105 98
94 94 91 86 84 77 72 71
70 70 63 44 39 38 31 30
29 24 22 18 15 9 6
BspHI T'CATG_A
Cuts at: 0 1163 1937 2264 2534 4757 5048 5186 6669
Size: 1163 774 327 270 2223 291 138 1483
Fragments arranged by size:
2223 1483 1163 774 327 291 270 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 336 608 2105 2573 2802 6669
Size: 336 272 1497 468 229 3867
Fragments arranged by size:
3867 1497 468 336 272 229
BsrBI CCG'CTC
Cuts at: 0 855 3570 4037 6187 6621 6669
Size: 855 2715 467 2150 434 48
Fragments arranged by size:
2715 2150 855 467 434 48
BsrDI GCAATG_nn'
Cuts at: 0 1512 2031 3288 4772 6669
Size: 1512 519 1257 1484 1897
Fragments arranged by size:
1897 1512 1484 1257 519
BsrFI r'CCGG_y
Cuts at: 0 1226 1385 1508 1682 3586 4310 4967 5296
Size: 1226 159 123 174 1904 724 657 329
Cuts at: 5296 5369 5506 6169 6669
Size: 73 137 663 500
Fragments arranged by size:
1904 1226 724 663 657 500 329 174
159 137 123 73
BsrGI T'GTAC_A
Cuts at: 0 4157 6669
Size: 4157 2512
BssSI C'ACGA_G
Cuts at: 0 1984 2254 6534 6669
Size: 1984 270 4280 135
Fragments arranged by size:
4280 1984 270 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 428 4845 5157 5541 5908 6669
Size: 428 4417 312 384 367 761
Fragments arranged by size:
4417 761 428 384 367 312
BstEII G'GTnAC_C
Cuts at: 0 4503 5993 6669
Size: 4503 1490 676
Fragments arranged by size:
4503 1490 676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 1272 2013 2583 2832 3564 3645 3801
Size: 387 885 741 570 249 732 81 156
Cuts at: 3801 4515 4542 5601 5694 6474 6591 6669
Size: 714 27 1059 93 780 117 78
Fragments arranged by size:
1059 885 780 741 732 714 570 387
249 156 117 93 81 78 27
BstYI r'GATC_y
Cuts at: 0 338 587 845 1310 2018 2447 2697 3797
Size: 338 249 258 465 708 429 250 1100
Cuts at: 3797 5748 5780 6669
Size: 1951 32 889
Fragments arranged by size:
1951 1100 889 708 465 429 338 258
250 249 32
Bsu36I CC'TnA_GG
Cuts at: 0 547 6669
Size: 547 6122
BtgI C'CryG_G
Cuts at: 0 65 235 380 1461 2273 2929 3062 3079
Size: 65 170 145 1081 812 656 133 17
Cuts at: 3079 3215 3333 5024 5265 6071 6305 6330 6513
Size: 136 118 1691 241 806 234 25 183
Cuts at: 6513 6542 6584 6669
Size: 29 42 85
Fragments arranged by size:
1691 1081 812 806 656 241 234 183
170 145 136 133 118 85 65 42
29 25 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3285 3543 6669
Size: 3285 258 3126
Fragments arranged by size:
3285 3126 258
BtsI GCAGTG_nn'
Cuts at: 0 613 2809 5909 6669
Size: 613 2196 3100 760
Fragments arranged by size:
3100 2196 760 613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4569 4604 6669
Size: 4569 35 2065
Fragments arranged by size:
4569 2065 35
DraIII CAC_nnn'GTG
Cuts at: 0 2761 3377 6669
Size: 2761 616 3292
Fragments arranged by size:
3292 2761 616
DrdI GACnn_nn'nnGTC
Cuts at: 0 2470 3445 3523 6669
Size: 2470 975 78 3146
Fragments arranged by size:
3146 2470 975 78
EaeI y'GGCC_r
Cuts at: 0 1274 1680 1943 2285 2573 3200 3635 4049
Size: 1274 406 263 342 288 627 435 414
Cuts at: 4049 4667 4802 5316 5646 5735 6210 6476 6486
Size: 618 135 514 330 89 475 266 10
Cuts at: 6486 6567 6669
Size: 81 102
Fragments arranged by size:
1274 627 618 514 475 435 414 406
342 330 288 266 263 135 102 89
81 10
EagI C'GGCC_G
Cuts at: 0 1680 5735 6210 6486 6567 6669
Size: 1680 4055 475 276 81 102
Fragments arranged by size:
4055 1680 475 276 102 81
EarI CTCTTCn'nnn_
Cuts at: 0 1557 2092 2317 2563 2651 3460 3517 3985
Size: 1557 535 225 246 88 809 57 468
Cuts at: 3985 4325 4879 4891 5017 6226 6409 6658 6669
Size: 340 554 12 126 1209 183 249 11
Fragments arranged by size:
1557 1209 809 554 535 468 340 249
246 225 183 126 88 57 12 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1780 1858 2182 2614 3016 6669
Size: 1780 78 324 432 402 3653
Fragments arranged by size:
3653 1780 432 402 324 78
EcoICRI GAG'CTC
Cuts at: 0 1742 2642 2915 4917 5247 6669
Size: 1742 900 273 2002 330 1422
Fragments arranged by size:
2002 1742 1422 900 330 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 490 742 1248 1258 2035 2074 2086
Size: 133 357 252 506 10 777 39 12
Cuts at: 2086 2310 2402 2656 2949 3264 3586 3895 4349
Size: 224 92 254 293 315 322 309 454
Cuts at: 4349 4390 4537 4564 4615 4627 4723 4807 5275
Size: 41 147 27 51 12 96 84 468
Cuts at: 5275 5844 6140 6207 6655 6669
Size: 569 296 67 448 14
Fragments arranged by size:
777 569 506 468 454 448 357 322
315 309 296 293 254 252 224 147
133 96 92 84 67 51 41 39
27 14 12 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 1339 1369 2455 2584 4099 5318 6022
Size: 886 453 30 1086 129 1515 1219 704
Cuts at: 6022 6155 6669
Size: 133 514
Fragments arranged by size:
1515 1219 1086 886 704 514 453 133
129 30
EcoO109I rG'GnC_Cy
Cuts at: 0 551 924 1374 1395 1410 1433 1778 2773
Size: 551 373 450 21 15 23 345 995
Cuts at: 2773 2791 3128 3480 3807 4818 5275 5585 5625
Size: 18 337 352 327 1011 457 310 40
Cuts at: 5625 6160 6216 6558 6595 6669
Size: 535 56 342 37 74
Fragments arranged by size:
1011 995 551 535 457 450 373 352
345 342 337 327 310 74 56 40
37 23 21 18 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5454 6669
Size: 5454 1215
FseI GG_CCGG'CC
Cuts at: 0 6173 6669
Size: 6173 496
FspI TGC'GCA
Cuts at: 0 1339 3832 4771 6669
Size: 1339 2493 939 1898
Fragments arranged by size:
2493 1898 1339 939
HaeII r_GCGC'y
Cuts at: 0 14 166 921 992 1254 1353 1392 2250
Size: 14 152 755 71 262 99 39 858
Cuts at: 2250 2895 3044 3227 3868 4368 4383 4635 5312
Size: 645 149 183 641 500 15 252 677
Cuts at: 5312 5376 6607 6669
Size: 64 1231 62
Fragments arranged by size:
1231 858 755 677 645 641 500 262
252 183 152 149 99 71 64 62
39 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 1089 1093 1121 1125 1461 1493
Size: 307 32 750 4 28 4 336 32
Cuts at: 1493 1786 1818 2626 2658 2878 2910 2995 3027
Size: 293 32 808 32 220 32 85 32
Cuts at: 3027 3782 3814 5401 5433 5455 5487 5572 5604
Size: 755 32 1587 32 22 32 85 32
Cuts at: 5604 6669
Size: 1065
Fragments arranged by size:
1587 1065 808 755 750 336 307 293
220 85 85 32 32 32 32 32
32 32 32 32 32 28 22 4
4
HincII GTy'rAC
Cuts at: 0 951 1956 6006 6669
Size: 951 1005 4050 663
Fragments arranged by size:
4050 1005 951 663
HindIII A'AGCT_T
Cuts at: 0 4841 6669
Size: 4841 1828
KasI G'GCGC_C
Cuts at: 0 162 917 1388 2246 3040 3223 3864 4364
Size: 162 755 471 858 794 183 641 500
Cuts at: 4364 5308 6603 6669
Size: 944 1295 66
Fragments arranged by size:
1295 944 858 794 755 641 500 471
183 162 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 2672 3644 6669
Size: 350 2322 972 3025
Fragments arranged by size:
3025 2322 972 350
MscI TGG'CCA
Cuts at: 0 1276 1945 2287 2575 4669 4804 6669
Size: 1276 669 342 288 2094 135 1865
Fragments arranged by size:
2094 1865 1276 669 342 288 135
MslI CAynn'nnrTG
Cuts at: 0 220 259 385 472 679 1004 1460 1612
Size: 220 39 126 87 207 325 456 152
Cuts at: 1612 1939 2041 2581 2830 3322 3643 3766 4276
Size: 327 102 540 249 492 321 123 510
Cuts at: 4276 4810 4834 5167 5692 6669
Size: 534 24 333 525 977
Fragments arranged by size:
977 540 534 525 510 492 456 333
327 325 321 249 220 207 152 126
123 102 87 39 24
MspA1I CmG'CkG
Cuts at: 0 49 332 713 938 1245 1655 1747 2693
Size: 49 283 381 225 307 410 92 946
Cuts at: 2693 2969 3064 3081 3217 3335 3716 4064 4395
Size: 276 95 17 136 118 381 348 331
Cuts at: 4395 4659 5090 5121 5547 6073 6266 6332 6544
Size: 264 431 31 426 526 193 66 212
Cuts at: 6544 6579 6669
Size: 35 90
Fragments arranged by size:
946 526 431 426 410 381 381 348
331 307 283 276 264 225 212 193
136 118 95 92 90 66 49 35
31 17
NaeI GCC'GGC
Cuts at: 0 1387 1510 1684 3588 4312 4969 5371 5508
Size: 1387 123 174 1904 724 657 402 137
Cuts at: 5508 6171 6669
Size: 663 498
Fragments arranged by size:
1904 1387 724 663 657 498 402 174
137 123
NarI GG'CG_CC
Cuts at: 0 163 918 1389 2247 3041 3224 3865 4365
Size: 163 755 471 858 794 183 641 500
Cuts at: 4365 5309 6604 6669
Size: 944 1295 65
Fragments arranged by size:
1295 944 858 794 755 641 500 471
183 163 65
NcoI C'CATG_G
Cuts at: 0 380 1461 2273 2929 5265 6305 6584 6669
Size: 380 1081 812 656 2336 1040 279 85
Fragments arranged by size:
2336 1081 1040 812 656 380 279 85
NgoMIV G'CCGG_C
Cuts at: 0 1385 1508 1682 3586 4310 4967 5369 5506
Size: 1385 123 174 1904 724 657 402 137
Cuts at: 5506 6169 6669
Size: 663 500
Fragments arranged by size:
1904 1385 724 663 657 500 402 174
137 123
NsiI A_TGCA'T
Cuts at: 0 2345 6669
Size: 2345 4324
NspI r_CATG'y
Cuts at: 0 258 294 2046 2301 4275 4975 6669
Size: 258 36 1752 255 1974 700 1694
Fragments arranged by size:
1974 1752 1694 700 258 255 36
PasI CC'CwG_GG
Cuts at: 0 408 1359 1398 2507 2838 6135 6669
Size: 408 951 39 1109 331 3297 534
Fragments arranged by size:
3297 1109 951 534 408 331 39
PciI A'CATG_T
Cuts at: 0 4271 6669
Size: 4271 2398
PflMI CCAn_nnn'nTGG
Cuts at: 0 1398 1611 2256 2761 4005 4464 5271 6519
Size: 1398 213 645 505 1244 459 807 1248
Cuts at: 6519 6669
Size: 150
Fragments arranged by size:
1398 1248 1244 807 645 505 459 213
150
PfoI T'CCnGG_A
Cuts at: 0 605 719 1735 2012 2672 2908 6156 6669
Size: 605 114 1016 277 660 236 3248 513
Fragments arranged by size:
3248 1016 660 605 513 277 236 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2398 2430 3457 3489 6669
Size: 2398 32 1027 32 3180
Fragments arranged by size:
3180 2398 1027 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 924 2773 2791 3480 3807 5585 5625 6160
Size: 924 1849 18 689 327 1778 40 535
Cuts at: 6160 6558 6669
Size: 398 111
Fragments arranged by size:
1849 1778 924 689 535 398 327 111
40 18
PshAI GACnn'nnGTC
Cuts at: 0 2747 5615 5822 6669
Size: 2747 2868 207 847
Fragments arranged by size:
2868 2747 847 207
PspOMI G'GGCC_C
Cuts at: 0 1401 1432 1778 3127 4817 5274 6594 6669
Size: 1401 31 346 1349 1690 457 1320 75
Fragments arranged by size:
1690 1401 1349 1320 457 346 75 31
PspXI vC'TCGA_Gb
Cuts at: 0 5248 6094 6669
Size: 5248 846 575
Fragments arranged by size:
5248 846 575
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 949 981 4141 4173 6669
Size: 949 32 3160 32 2496
Fragments arranged by size:
3160 2496 949 32 32
PstI C_TGCA'G
Cuts at: 0 36 347 1382 2816 5108 5904 5972 6086
Size: 36 311 1035 1434 2292 796 68 114
Cuts at: 6086 6669
Size: 583
Fragments arranged by size:
2292 1434 1035 796 583 311 114 68
36
PvuII CAG'CTG
Cuts at: 0 1655 2969 3716 4659 5090 5547 6669
Size: 1655 1314 747 943 431 457 1122
Fragments arranged by size:
1655 1314 1122 943 747 457 431
RsrII CG'GwC_CG
Cuts at: 0 6546 6669
Size: 6546 123
SacI G_AGCT'C
Cuts at: 0 1744 2644 2917 4919 5249 6669
Size: 1744 900 273 2002 330 1420
Fragments arranged by size:
2002 1744 1420 900 330 273
SacII CC_GC'GG
Cuts at: 0 3065 3082 3218 3336 6074 6333 6545 6669
Size: 3065 17 136 118 2738 259 212 124
Fragments arranged by size:
3065 2738 259 212 136 124 118 17
SapI GCTCTTCn'nnn_
Cuts at: 0 2092 2317 2563 3985 4891 6669
Size: 2092 225 246 1422 906 1778
Fragments arranged by size:
2092 1778 1422 906 246 225
SbfI CC_TGCA'GG
Cuts at: 0 347 6669
Size: 347 6322
ScaI AGT'ACT
Cuts at: 0 493 1921 4561 6669
Size: 493 1428 2640 2108
Fragments arranged by size:
2640 2108 1428 493
SfcI C'TryA_G
Cuts at: 0 32 343 1378 2812 5104 5900 5968 6082
Size: 32 311 1035 1434 2292 796 68 114
Cuts at: 6082 6669
Size: 587
Fragments arranged by size:
2292 1434 1035 796 587 311 114 68
32
SfoI GGC'GCC
Cuts at: 0 164 919 1390 2248 3042 3225 3866 4366
Size: 164 755 471 858 794 183 641 500
Cuts at: 4366 5310 6605 6669
Size: 944 1295 64
Fragments arranged by size:
1295 944 858 794 755 641 500 471
183 164 64
SmaI CCC'GGG
Cuts at: 0 3046 3235 3267 5306 6166 6284 6669
Size: 3046 189 32 2039 860 118 385
Fragments arranged by size:
3046 2039 860 385 189 118 32
SmlI C'TyrA_G
Cuts at: 0 394 856 2980 3907 4555 5248 5620 6094
Size: 394 462 2124 927 648 693 372 474
Cuts at: 6094 6669
Size: 575
Fragments arranged by size:
2124 927 693 648 575 474 462 394
372
SphI G_CATG'C
Cuts at: 0 258 2301 4975 6669
Size: 258 2043 2674 1694
Fragments arranged by size:
2674 2043 1694 258
SrfI GCCC'GGGC
Cuts at: 0 6284 6669
Size: 6284 385
StuI AGG'CCT
Cuts at: 0 110 2681 5563 6669
Size: 110 2571 2882 1106
Fragments arranged by size:
2882 2571 1106 110
StyI C'CwwG_G
Cuts at: 0 380 887 1442 1461 2273 2334 2769 2929
Size: 380 507 555 19 812 61 435 160
Cuts at: 2929 4133 5265 5438 5998 6305 6584 6599 6669
Size: 1204 1132 173 560 307 279 15 70
Fragments arranged by size:
1204 1132 812 560 555 507 435 380
307 279 173 160 70 61 19 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 167 1065 2470 2749 4134 4270 4661 5089
Size: 167 898 1405 279 1385 136 391 428
Cuts at: 5089 5593 6307 6669
Size: 504 714 362
Fragments arranged by size:
1405 1385 898 714 504 428 391 362
279 167 136
TatI w'GTAC_w
Cuts at: 0 491 1088 1919 3683 4157 4226 4559 5123
Size: 491 597 831 1764 474 69 333 564
Cuts at: 5123 6669
Size: 1546
Fragments arranged by size:
1764 1546 831 597 564 491 474 333
69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2375 2407 5386 5418 5479 5511 6286 6318
Size: 2375 32 2979 32 61 32 775 32
Cuts at: 6318 6669
Size: 351
Fragments arranged by size:
2979 2375 775 351 61 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 895 3168 3520 5413 6669
Size: 895 2273 352 1893 1256
Fragments arranged by size:
2273 1893 1256 895 352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1525 2280 2736 2754 4461 6669
Size: 1525 755 456 18 1707 2208
Fragments arranged by size:
2208 1707 1525 755 456 18
XhoI C'TCGA_G
Cuts at: 0 5248 5620 6094 6669
Size: 5248 372 474 575
Fragments arranged by size:
5248 575 474 372
XmaI C'CCGG_G
Cuts at: 0 3044 3233 3265 5304 6164 6282 6669
Size: 3044 189 32 2039 860 118 387
Fragments arranged by size:
3044 2039 860 387 189 118 32
XmnI GAAnn'nnTTC
Cuts at: 0 2407 3472 6669
Size: 2407 1065 3197
Fragments arranged by size:
3197 2407 1065
ZraI GAC'GTC
Cuts at: 0 897 3312 3405 6669
Size: 897 2415 93 3264
Fragments arranged by size:
3264 2415 897 93
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
RsrII SacI SacII SapI SbfI ScaI SfcI SfoI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI ApoI AscI AseI
AsiSI BaeI BciVI BmtI BspEI BssHII BstBI BstZ17I
ClaI DraI EcoRI EcoRV FspAI HpaI KpnI MfeI
MluI NdeI NheI NotI NruI PacI PmeI PmlI
PsiI PvuI SalI SanDI SexAI SfiI SgfI SgrAI
SnaBI SpeI SspI SwaI XbaI