Restriction map for
pFN21AE2482
(Linear) (Six-base) MAPSORT of: KIEE2482.seq Check: 5803 from: 1 to: 1404
KIEE2482.orf
With 182 enzymes: SgfI *
September 20, 2013 16:32 ..
AatII G_ACGT'C
Cuts at: 0 1004 1404
Size: 1004 400
AccI GT'mk_AC
Cuts at: 0 1258 1404
Size: 1258 146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1355 1404
Size: 1103 252 49
Fragments arranged by size:
1103 252 49
AflII C'TTAA_G
Cuts at: 0 1263 1404
Size: 1263 141
AflIII A'CryG_T
Cuts at: 0 100 1197 1404
Size: 100 1097 207
Fragments arranged by size:
1097 207 100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 161 818 1404
Size: 161 657 586
Fragments arranged by size:
657 586 161
ApoI r'AATT_y
Cuts at: 0 265 1048 1178 1375 1404
Size: 265 783 130 197 29
Fragments arranged by size:
783 265 197 130 29
AvaI C'yCGr_G
Cuts at: 0 251 1404
Size: 251 1153
BanII G_rGCy'C
Cuts at: 0 719 1404
Size: 719 685
BbsI GAAGACnn'nnnn_
Cuts at: 0 996 1031 1404
Size: 996 35 373
Fragments arranged by size:
996 373 35
BclI T'GATC_A
Cuts at: 0 885 1404
Size: 885 519
BglII A'GATC_T
Cuts at: 0 56 1404
Size: 56 1348
BlpI GC'TnA_GC
Cuts at: 0 371 1404
Size: 371 1033
Bme1580I G_kGCm'C
Cuts at: 0 455 1404
Size: 455 949
BmgBI CAC'GTC
Cuts at: 0 1200 1404
Size: 1200 204
BmtI G_CTAG'C
Cuts at: 0 1189 1404
Size: 1189 215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 1404
Size: 733 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 703 1404
Size: 383 320 701
Fragments arranged by size:
701 383 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1404
Size: 726 678
BsaHI Gr'CG_yC
Cuts at: 0 382 1001 1404
Size: 382 619 403
Fragments arranged by size:
619 403 382
BsaWI w'CCGG_w
Cuts at: 0 323 1404
Size: 323 1081
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1404
Size: 775 30 599
Fragments arranged by size:
775 599 30
BsiHKAI G_wGCw'C
Cuts at: 0 719 1404
Size: 719 685
Bsp1286I G_dGCh'C
Cuts at: 0 455 719 1404
Size: 455 264 685
Fragments arranged by size:
685 455 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 703 898 1404
Size: 703 195 506
Fragments arranged by size:
703 506 195
BsrDI GCAATG_nn'
Cuts at: 0 150 1394 1404
Size: 150 1244 10
Fragments arranged by size:
1244 150 10
BstYI r'GATC_y
Cuts at: 0 56 1404
Size: 56 1348
Bsu36I CC'TnA_GG
Cuts at: 0 461 1014 1404
Size: 461 553 390
Fragments arranged by size:
553 461 390
BtgI C'CryG_G
Cuts at: 0 641 1404
Size: 641 763
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 37 1404
Size: 37 1367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 42 77 1404
Size: 42 35 1327
Fragments arranged by size:
1327 42 35
DraI TTT'AAA
Cuts at: 0 1308 1404
Size: 1308 96
DrdI GACnn_nn'nnGTC
Cuts at: 0 484 1404
Size: 484 920
EaeI y'GGCC_r
Cuts at: 0 270 401 1404
Size: 270 131 1003
Fragments arranged by size:
1003 270 131
EarI CTCTTCn'nnn_
Cuts at: 0 707 1196 1404
Size: 707 489 208
Fragments arranged by size:
707 489 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 471 1404
Size: 471 933
EcoICRI GAG'CTC
Cuts at: 0 717 1404
Size: 717 687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 703 1103 1355 1404
Size: 383 320 400 252 49
Fragments arranged by size:
400 383 320 252 49
EcoRV GAT'ATC
Cuts at: 0 540 1404
Size: 540 864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 58 90 1404
Size: 15 32 11 32 1314
Fragments arranged by size:
1314 32 32 15 11
MfeI C'AATT_G
Cuts at: 0 796 1404
Size: 796 608
MslI CAynn'nnrTG
Cuts at: 0 89 1404
Size: 89 1315
MspA1I CmG'CkG
Cuts at: 0 206 378 780 1404
Size: 206 172 402 624
Fragments arranged by size:
624 402 206 172
NcoI C'CATG_G
Cuts at: 0 641 1404
Size: 641 763
NheI G'CTAG_C
Cuts at: 0 1185 1404
Size: 1185 219
NspI r_CATG'y
Cuts at: 0 104 1404
Size: 104 1300
PciI A'CATG_T
Cuts at: 0 100 1404
Size: 100 1304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1404
Size: 1050 32 322
Fragments arranged by size:
1050 322 32
SacI G_AGCT'C
Cuts at: 0 719 1404
Size: 719 685
SapI GCTCTTCn'nnn_
Cuts at: 0 707 1196 1404
Size: 707 489 208
Fragments arranged by size:
707 489 208
SfcI C'TryA_G
Cuts at: 0 1204 1404
Size: 1204 200
SmlI C'TyrA_G
Cuts at: 0 251 705 1263 1404
Size: 251 454 558 141
Fragments arranged by size:
558 454 251 141
StyI C'CwwG_G
Cuts at: 0 641 1404
Size: 641 763
TatI w'GTAC_w
Cuts at: 0 312 971 1113 1404
Size: 312 659 142 291
Fragments arranged by size:
659 312 291 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 1404
Size: 775 32 597
Fragments arranged by size:
775 597 32
XbaI T'CTAG_A
Cuts at: 0 915 1404
Size: 915 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 358 1404
Size: 358 1046
XhoI C'TCGA_G
Cuts at: 0 251 1404
Size: 251 1153
XmnI GAAnn'nnTTC
Cuts at: 0 44 1404
Size: 44 1360
ZraI GAC'GTC
Cuts at: 0 1002 1404
Size: 1002 402
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlfI ApoI AvaI
BanII BbsI BclI BglII BlpI Bme1580I BmgBI BmtI
BplI BpmI BpuEI BsaHI BsaWI BsaXI BsiHKAI Bsp1286I
BspMI BsrDI BstYI Bsu36I BtgI BtgZI CspCI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoRV Hin4I
MfeI MslI MspA1I NcoI NheI NspI PciI PpiI
SacI SapI SfcI SmlI StyI TatI TstI XbaI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BmrI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtsI ClaI DraIII EagI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XmaI