Restriction map for
pFN21AE2475
(Linear) (Six-base) MAPSORT of: KIEE2475.seq Check: 9033 from: 1 to: 1092
KIEE2475.orf
With 182 enzymes: SgfI *
May 14, 2013 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 884 931 1006 1025 1092
Size: 149 735 47 75 19 67
Fragments arranged by size:
735 149 75 67 47 19
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1092
Size: 1057 32 3
Fragments arranged by size:
1057 32 3
ApaLI G'TGCA_C
Cuts at: 0 431 1092
Size: 431 661
ApoI r'AATT_y
Cuts at: 0 42 586 947 1092
Size: 42 544 361 145
Fragments arranged by size:
544 361 145 42
AvrII C'CTAG_G
Cuts at: 0 732 1092
Size: 732 360
BamHI G'GATC_C
Cuts at: 0 75 1092
Size: 75 1017
BanII G_rGCy'C
Cuts at: 0 787 1092
Size: 787 305
BclI T'GATC_A
Cuts at: 0 170 1092
Size: 170 922
BfrBI ATG'CAT
Cuts at: 0 694 1092
Size: 694 398
Bme1580I G_kGCm'C
Cuts at: 0 435 1092
Size: 435 657
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 1092
Size: 329 763
Bpu10I CC'TnA_GC
Cuts at: 0 614 1092
Size: 614 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 963 1092
Size: 833 130 129
Fragments arranged by size:
833 130 129
BsaBI GATnn'nnATC
Cuts at: 0 1035 1092
Size: 1035 57
BsaWI w'CCGG_w
Cuts at: 0 744 1092
Size: 744 348
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 747 1092
Size: 565 182 345
Fragments arranged by size:
565 345 182
BseYI C'CCAG_C
Cuts at: 0 786 1092
Size: 786 306
BsiHKAI G_wGCw'C
Cuts at: 0 435 849 1092
Size: 435 414 243
Fragments arranged by size:
435 414 243
BsmI GAATG_Cn'
Cuts at: 0 204 1015 1051 1092
Size: 204 811 36 41
Fragments arranged by size:
811 204 41 36
Bsp1286I G_dGCh'C
Cuts at: 0 435 787 849 1092
Size: 435 352 62 243
Fragments arranged by size:
435 352 243 62
BspEI T'CCGG_A
Cuts at: 0 744 1092
Size: 744 348
BspHI T'CATG_A
Cuts at: 0 417 1092
Size: 417 675
BsrDI GCAATG_nn'
Cuts at: 0 20 1020 1092
Size: 20 1000 72
Fragments arranged by size:
1000 72 20
BsrFI r'CCGG_y
Cuts at: 0 377 1092
Size: 377 715
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 1092
Size: 199 893
BstEII G'GTnAC_C
Cuts at: 0 339 1092
Size: 339 753
BstYI r'GATC_y
Cuts at: 0 75 1092
Size: 75 1017
BtsI GCAGTG_nn'
Cuts at: 0 635 1092
Size: 635 457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1040 1075 1092
Size: 1040 35 17
Fragments arranged by size:
1040 35 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 884 931 1006 1025 1092
Size: 149 735 47 75 19 67
Fragments arranged by size:
735 149 75 67 47 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 1092
Size: 217 875
EcoO109I rG'GnC_Cy
Cuts at: 0 133 1092
Size: 133 959
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 238 270 298 330 1092
Size: 9 32 197 32 28 32 762
Fragments arranged by size:
762 197 32 32 32 28 9
HincII GTy'rAC
Cuts at: 0 827 1092
Size: 827 265
HindIII A'AGCT_T
Cuts at: 0 925 1092
Size: 925 167
HpaI GTT'AAC
Cuts at: 0 827 1092
Size: 827 265
MslI CAynn'nnrTG
Cuts at: 0 409 1092
Size: 409 683
MspA1I CmG'CkG
Cuts at: 0 646 832 1092
Size: 646 186 260
Fragments arranged by size:
646 260 186
NsiI A_TGCA'T
Cuts at: 0 696 1092
Size: 696 396
NspI r_CATG'y
Cuts at: 0 147 845 1092
Size: 147 698 247
Fragments arranged by size:
698 247 147
PfoI T'CCnGG_A
Cuts at: 0 78 1092
Size: 78 1014
PpuMI rG'GwC_Cy
Cuts at: 0 133 1092
Size: 133 959
PvuII CAG'CTG
Cuts at: 0 646 1092
Size: 646 446
SexAI A'CCwGG_T
Cuts at: 0 153 1092
Size: 153 939
SmlI C'TyrA_G
Cuts at: 0 848 942 1092
Size: 848 94 150
Fragments arranged by size:
848 150 94
StyI C'CwwG_G
Cuts at: 0 204 218 669 732 791 1092
Size: 204 14 451 63 59 301
Fragments arranged by size:
451 301 204 63 59 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 516 548 1092
Size: 74 32 410 32 544
Fragments arranged by size:
544 410 74 32 32
Enzymes that do cut:
AcuI AloI ApaLI ApoI AvrII BamHI BanII BclI
BfrBI Bme1580I BmrI Bpu10I BpuEI BsaBI BsaWI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI
BstAPI BstEII BstYI BtsI CspCI Eco57MI EcoNI EcoO109I
Hin4I HincII HindIII HpaI MslI MspA1I NsiI NspI
PfoI PpuMI PvuII SexAI SmlI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI AscI AseI
AsiSI AvaI BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI BmgBI BmtI BplI BpmI
BsaI BsaAI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI
BspMI BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI