Restriction map for
pFN21AE2460
(Linear) (Six-base) MAPSORT of: KIEE2460.seq Check: 2317 from: 1 to: 1695
KIEE2460.orf
With 182 enzymes: SgfI *
March 29, 2013 15:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1333 1695
Size: 1333 362
Acc65I G'GTAC_C
Cuts at: 0 820 1695
Size: 820 875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 773 1695
Size: 304 469 922
Fragments arranged by size:
922 469 304
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 726 759 1695
Size: 726 33 936
Fragments arranged by size:
936 726 33
BanI G'GyrC_C
Cuts at: 0 449 820 1513 1695
Size: 449 371 693 182
Fragments arranged by size:
693 449 371 182
BanII G_rGCy'C
Cuts at: 0 147 1013 1695
Size: 147 866 682
Fragments arranged by size:
866 682 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 898 1599 1695
Size: 898 701 96
Fragments arranged by size:
898 701 96
BciVI GTATCCnnnnn_n'
Cuts at: 0 39 727 1695
Size: 39 688 968
Fragments arranged by size:
968 688 39
BfrBI ATG'CAT
Cuts at: 0 1428 1695
Size: 1428 267
BglII A'GATC_T
Cuts at: 0 264 1695
Size: 264 1431
Bme1580I G_kGCm'C
Cuts at: 0 390 460 1518 1695
Size: 390 70 1058 177
Fragments arranged by size:
1058 390 177 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 1329 1646 1695
Size: 1329 317 49
Fragments arranged by size:
1329 317 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1474 1578 1695
Size: 1076 398 104 117
Fragments arranged by size:
1076 398 117 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1695
Size: 366 1329
BsaAI yAC'GTr
Cuts at: 0 703 1695
Size: 703 992
BsaWI w'CCGG_w
Cuts at: 0 1054 1695
Size: 1054 641
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 483 513 1695
Size: 483 30 1182
Fragments arranged by size:
1182 483 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1160 1223 1695
Size: 1160 63 472
Fragments arranged by size:
1160 472 63
BseYI C'CCAG_C
Cuts at: 0 134 140 1338 1695
Size: 134 6 1198 357
Fragments arranged by size:
1198 357 134 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 693 1238 1379 1459 1695
Size: 457 236 545 141 80 236
Fragments arranged by size:
545 457 236 236 141 80
BsiEI CG_ry'CG
Cuts at: 0 53 1419 1695
Size: 53 1366 276
Fragments arranged by size:
1366 276 53
BsiHKAI G_wGCw'C
Cuts at: 0 1013 1695
Size: 1013 682
BsiWI C'GTAC_G
Cuts at: 0 699 1695
Size: 699 996
BsmI GAATG_Cn'
Cuts at: 0 655 1428 1695
Size: 655 773 267
Fragments arranged by size:
773 655 267
Bsp1286I G_dGCh'C
Cuts at: 0 147 390 460 1013 1518 1695
Size: 147 243 70 553 505 177
Fragments arranged by size:
553 505 243 177 147 70
BspHI T'CATG_A
Cuts at: 0 636 1695
Size: 636 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 403 1333 1695
Size: 50 353 930 362
Fragments arranged by size:
930 362 353 50
BsrDI GCAATG_nn'
Cuts at: 0 559 1695
Size: 559 1136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1078 1695
Size: 1078 617
BstXI CCAn_nnnn'nTGG
Cuts at: 0 745 1695
Size: 745 950
BstYI r'GATC_y
Cuts at: 0 264 1695
Size: 264 1431
BtgI C'CryG_G
Cuts at: 0 401 1162 1275 1518 1551 1695
Size: 401 761 113 243 33 144
Fragments arranged by size:
761 401 243 144 113 33
BtsI GCAGTG_nn'
Cuts at: 0 1677 1695
Size: 1677 18
ClaI AT'CG_AT
Cuts at: 0 94 1695
Size: 94 1601
DraI TTT'AAA
Cuts at: 0 830 1695
Size: 830 865
DraIII CAC_nnn'GTG
Cuts at: 0 1464 1695
Size: 1464 231
EaeI y'GGCC_r
Cuts at: 0 50 1416 1548 1695
Size: 50 1366 132 147
Fragments arranged by size:
1366 147 132 50
EagI C'GGCC_G
Cuts at: 0 50 1416 1695
Size: 50 1366 279
Fragments arranged by size:
1366 279 50
EarI CTCTTCn'nnn_
Cuts at: 0 522 1695
Size: 522 1173
EcoICRI GAG'CTC
Cuts at: 0 1011 1695
Size: 1011 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 773 1076 1474 1578 1695
Size: 304 469 303 398 104 117
Fragments arranged by size:
469 398 304 303 117 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1094 1695
Size: 1094 601
EcoO109I rG'GnC_Cy
Cuts at: 0 494 707 1335 1437 1695
Size: 494 213 628 102 258
Fragments arranged by size:
628 494 258 213 102
HaeII r_GCGC'y
Cuts at: 0 19 1695
Size: 19 1676
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 344 376 637 669 1116 1148 1695
Size: 344 32 261 32 447 32 547
Fragments arranged by size:
547 447 344 261 32 32 32
HincII GTy'rAC
Cuts at: 0 561 1110 1695
Size: 561 549 585
Fragments arranged by size:
585 561 549
KpnI G_GTAC'C
Cuts at: 0 824 1695
Size: 824 871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1695
Size: 849 846
MscI TGG'CCA
Cuts at: 0 1550 1695
Size: 1550 145
MspA1I CmG'CkG
Cuts at: 0 1078 1695
Size: 1078 617
NcoI C'CATG_G
Cuts at: 0 401 1275 1551 1695
Size: 401 874 276 144
Fragments arranged by size:
874 401 276 144
NotI GC'GGCC_GC
Cuts at: 0 50 1695
Size: 50 1645
NsiI A_TGCA'T
Cuts at: 0 1430 1695
Size: 1430 265
PasI CC'CwG_GG
Cuts at: 0 1381 1695
Size: 1381 314
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 1345 1695
Size: 466 879 350
Fragments arranged by size:
879 466 350
PfoI T'CCnGG_A
Cuts at: 0 1092 1695
Size: 1092 603
PpuMI rG'GwC_Cy
Cuts at: 0 494 707 1335 1437 1695
Size: 494 213 628 102 258
Fragments arranged by size:
628 494 258 213 102
PsiI TTA'TAA
Cuts at: 0 695 1695
Size: 695 1000
PstI C_TGCA'G
Cuts at: 0 47 1695
Size: 47 1648
PvuII CAG'CTG
Cuts at: 0 1078 1695
Size: 1078 617
SacI G_AGCT'C
Cuts at: 0 1013 1695
Size: 1013 682
SanDI GG'GwC_CC
Cuts at: 0 1335 1695
Size: 1335 360
SapI GCTCTTCn'nnn_
Cuts at: 0 522 1695
Size: 522 1173
SbfI CC_TGCA'GG
Cuts at: 0 47 1695
Size: 47 1648
ScaI AGT'ACT
Cuts at: 0 876 1695
Size: 876 819
SfcI C'TryA_G
Cuts at: 0 43 521 1067 1695
Size: 43 478 546 628
Fragments arranged by size:
628 546 478 43
SmlI C'TyrA_G
Cuts at: 0 381 1695
Size: 381 1314
SspI AAT'ATT
Cuts at: 0 1193 1695
Size: 1193 502
StuI AGG'CCT
Cuts at: 0 927 1695
Size: 927 768
StyI C'CwwG_G
Cuts at: 0 401 1275 1551 1695
Size: 401 874 276 144
Fragments arranged by size:
874 401 276 144
TatI w'GTAC_w
Cuts at: 0 874 1084 1695
Size: 874 210 611
Fragments arranged by size:
874 611 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 483 515 1695
Size: 483 32 1180
Fragments arranged by size:
1180 483 32
Tth111I GACn'n_nGTC
Cuts at: 0 164 1362 1695
Size: 164 1198 333
Fragments arranged by size:
1198 333 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1282 1695
Size: 1282 413
Enzymes that do cut:
AarI Acc65I AcuI BaeI BanI BanII BbsI BciVI
BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspHI BspMI BsrDI BstAPI BstXI BstYI BtgI
BtsI ClaI DraI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI
MscI MspA1I NcoI NotI NsiI PasI PflMI PfoI
PpuMI PsiI PstI PvuII SacI SanDI SapI SbfI
ScaI SfcI SmlI SspI StuI StyI TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI
BclI BglI BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaHI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DrdI
EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NruI NspI PacI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII XbaI XhoI XmaI
XmnI ZraI