Restriction map for
pFN21AE2459
(Linear) (Six-base) MAPSORT of: KIEE2459.seq Check: 8301 from: 1 to: 1515
KIEE2459.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 602 1187 1515
Size: 406 196 585 328
Fragments arranged by size:
585 406 328 196
AflIII A'CryG_T
Cuts at: 0 130 1515
Size: 130 1385
AlwNI CAG_nnn'CTG
Cuts at: 0 1355 1515
Size: 1355 160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 146 179 461 494 1515
Size: 146 33 282 33 1021
Fragments arranged by size:
1021 282 146 33 33
BclI T'GATC_A
Cuts at: 0 207 867 1515
Size: 207 660 648
Fragments arranged by size:
660 648 207
BfrBI ATG'CAT
Cuts at: 0 702 1515
Size: 702 813
BglI GCCn_nnn'nGGC
Cuts at: 0 977 1515
Size: 977 538
BglII A'GATC_T
Cuts at: 0 1234 1515
Size: 1234 281
BlpI GC'TnA_GC
Cuts at: 0 530 1515
Size: 530 985
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1256 1515
Size: 1256 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1515
Size: 128 1387
BsaAI yAC'GTr
Cuts at: 0 131 1515
Size: 131 1384
BsaBI GATnn'nnATC
Cuts at: 0 866 1092 1515
Size: 866 226 423
Fragments arranged by size:
866 423 226
BsaHI Gr'CG_yC
Cuts at: 0 30 1515
Size: 30 1485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 749 779 1515
Size: 749 30 736
Fragments arranged by size:
749 736 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 1515
Size: 409 1106
BseYI C'CCAG_C
Cuts at: 0 1111 1515
Size: 1111 404
BsiHKAI G_wGCw'C
Cuts at: 0 1448 1515
Size: 1448 67
BsmI GAATG_Cn'
Cuts at: 0 1464 1515
Size: 1464 51
Bsp1286I G_dGCh'C
Cuts at: 0 1448 1515
Size: 1448 67
BspHI T'CATG_A
Cuts at: 0 1440 1473 1515
Size: 1440 33 42
Fragments arranged by size:
1440 42 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1207 1515
Size: 1207 308
BstYI r'GATC_y
Cuts at: 0 1234 1515
Size: 1234 281
BtsI GCAGTG_nn'
Cuts at: 0 482 1515
Size: 482 1033
ClaI AT'CG_AT
Cuts at: 0 1002 1515
Size: 1002 513
DraIII CAC_nnn'GTG
Cuts at: 0 1452 1515
Size: 1452 63
EaeI y'GGCC_r
Cuts at: 0 802 1491 1515
Size: 802 689 24
Fragments arranged by size:
802 689 24
EarI CTCTTCn'nnn_
Cuts at: 0 578 1515
Size: 578 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 958 1515
Size: 958 557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 406 602 1187 1515
Size: 128 278 196 585 328
Fragments arranged by size:
585 328 278 196 128
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 1515
Size: 391 1124
EcoO109I rG'GnC_Cy
Cuts at: 0 979 1391 1515
Size: 979 412 124
Fragments arranged by size:
979 412 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 526 1515
Size: 526 989
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 968 984 1000 1016 1278 1310
Size: 103 32 833 16 16 16 262 32
Cuts at: 1310 1515
Size: 205
Fragments arranged by size:
833 262 205 103 32 32 16 16
16
HincII GTy'rAC
Cuts at: 0 1363 1515
Size: 1363 152
MfeI C'AATT_G
Cuts at: 0 481 1515
Size: 481 1034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1515
Size: 272 1243
MscI TGG'CCA
Cuts at: 0 1493 1515
Size: 1493 22
MslI CAynn'nnrTG
Cuts at: 0 1187 1515
Size: 1187 328
MspA1I CmG'CkG
Cuts at: 0 106 1515
Size: 106 1409
NsiI A_TGCA'T
Cuts at: 0 704 1515
Size: 704 811
NspI r_CATG'y
Cuts at: 0 52 706 926 1154 1515
Size: 52 654 220 228 361
Fragments arranged by size:
654 361 228 220 52
PfoI T'CCnGG_A
Cuts at: 0 1308 1515
Size: 1308 207
PmlI CAC'GTG
Cuts at: 0 131 1515
Size: 131 1384
PshAI GACnn'nnGTC
Cuts at: 0 1358 1515
Size: 1358 157
PsiI TTA'TAA
Cuts at: 0 494 1515
Size: 494 1021
PstI C_TGCA'G
Cuts at: 0 105 1515
Size: 105 1410
PvuII CAG'CTG
Cuts at: 0 106 1515
Size: 106 1409
SfcI C'TryA_G
Cuts at: 0 101 732 1515
Size: 101 631 783
Fragments arranged by size:
783 631 101
SphI G_CATG'C
Cuts at: 0 926 1515
Size: 926 589
StyI C'CwwG_G
Cuts at: 0 845 1515
Size: 845 670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 23 1515
Size: 23 1492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 81 113 1515
Size: 81 32 1402
Fragments arranged by size:
1402 81 32
XbaI T'CTAG_A
Cuts at: 0 1126 1515
Size: 1126 389
Enzymes that do cut:
AcuI AflIII AlwNI BaeI BclI BfrBI BglI BglII
BlpI BplI BpmI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BstXI BstYI BtsI
ClaI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NsiI NspI PfoI PmlI PshAI PsiI PstI PvuII
SfcI SphI StyI TaqII TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI Bme1580I BmgBI BmrI BmtI
Bpu10I BpuEI BsaI BsaWI BsgI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XcmI XhoI XmaI XmnI ZraI