Restriction map for
pFN21AE2447
(Linear) (Six-base) MAPSORT of: KIEE2447.seq Check: 5826 from: 1 to: 2556
KIEE2447.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1375 2556
Size: 1375 1181
AccI GT'mk_AC
Cuts at: 0 468 2377 2556
Size: 468 1909 179
Fragments arranged by size:
1909 468 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 902 1690 2207 2413 2556
Size: 77 825 788 517 206 143
Fragments arranged by size:
825 788 517 206 143 77
AhdI GACnn_n'nnGTC
Cuts at: 0 2430 2556
Size: 2430 126
AleI CACnn'nnGTG
Cuts at: 0 2413 2556
Size: 2413 143
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1901 1933 2556
Size: 1901 32 623
Fragments arranged by size:
1901 623 32
ApaI G_GGCC'C
Cuts at: 0 615 2556
Size: 615 1941
ApaLI G'TGCA_C
Cuts at: 0 798 2556
Size: 798 1758
ApoI r'AATT_y
Cuts at: 0 63 330 396 733 1118 1604 2048 2306
Size: 63 267 66 337 385 486 444 258
Cuts at: 2306 2505 2556
Size: 199 51
Fragments arranged by size:
486 444 385 337 267 258 199 66
63 51
AvaI C'yCGr_G
Cuts at: 0 1741 2556
Size: 1741 815
BamHI G'GATC_C
Cuts at: 0 894 2556
Size: 894 1662
BanII G_rGCy'C
Cuts at: 0 604 615 811 2556
Size: 604 11 196 1745
Fragments arranged by size:
1745 604 196 11
BclI T'GATC_A
Cuts at: 0 2043 2556
Size: 2043 513
BglII A'GATC_T
Cuts at: 0 406 2556
Size: 406 2150
Bme1580I G_kGCm'C
Cuts at: 0 615 710 802 2556
Size: 615 95 92 1754
Fragments arranged by size:
1754 615 95 92
BmgBI CAC'GTC
Cuts at: 0 2194 2556
Size: 2194 362
BmrI ACTGGGnnnn_n'
Cuts at: 0 1139 2556
Size: 1139 1417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1223 2268 2348 2403 2493 2507 2556
Size: 883 340 1045 80 55 90 14 49
Fragments arranged by size:
1045 883 340 90 80 55 49 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 1038 2556
Size: 375 663 1518
Fragments arranged by size:
1518 663 375
BsaBI GATnn'nnATC
Cuts at: 0 365 2042 2556
Size: 365 1677 514
Fragments arranged by size:
1677 514 365
BsaWI w'CCGG_w
Cuts at: 0 346 2556
Size: 346 2210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 656 686 2556
Size: 656 30 1870
Fragments arranged by size:
1870 656 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1729 2556
Size: 1729 827
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 2556
Size: 1031 1525
BsiHKAI G_wGCw'C
Cuts at: 0 802 1430 2391 2556
Size: 802 628 961 165
Fragments arranged by size:
961 802 628 165
BsmI GAATG_Cn'
Cuts at: 0 121 2533 2556
Size: 121 2412 23
Fragments arranged by size:
2412 121 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2200 2556
Size: 2200 356
Bsp1286I G_dGCh'C
Cuts at: 0 604 615 710 802 811 1430 2391 2556
Size: 604 11 95 92 9 619 961 165
Fragments arranged by size:
961 619 604 165 95 92 11 9
BspHI T'CATG_A
Cuts at: 0 911 2556
Size: 911 1645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1375 2367 2556
Size: 1375 992 189
Fragments arranged by size:
1375 992 189
BsrBI CCG'CTC
Cuts at: 0 569 2556
Size: 569 1987
BsrGI T'GTAC_A
Cuts at: 0 1824 2556
Size: 1824 732
BstEII G'GTnAC_C
Cuts at: 0 633 1662 2556
Size: 633 1029 894
Fragments arranged by size:
1029 894 633
BstYI r'GATC_y
Cuts at: 0 294 406 485 894 2556
Size: 294 112 79 409 1662
Fragments arranged by size:
1662 409 294 112 79
BstZ17I GTA'TAC
Cuts at: 0 469 2378 2556
Size: 469 1909 178
Fragments arranged by size:
1909 469 178
BtsI GCAGTG_nn'
Cuts at: 0 1682 1827 2556
Size: 1682 145 729
Fragments arranged by size:
1682 729 145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1095 1130 2556
Size: 1095 35 1426
Fragments arranged by size:
1426 1095 35
DraI TTT'AAA
Cuts at: 0 960 1928 2556
Size: 960 968 628
Fragments arranged by size:
968 960 628
EaeI y'GGCC_r
Cuts at: 0 145 2556
Size: 145 2411
EarI CTCTTCn'nnn_
Cuts at: 0 259 1067 2165 2556
Size: 259 808 1098 391
Fragments arranged by size:
1098 808 391 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 758 2556
Size: 758 1798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 883 902 1223 1690 2207 2268 2348
Size: 77 806 19 321 467 517 61 80
Cuts at: 2348 2403 2413 2493 2507 2556
Size: 55 10 80 14 49
Fragments arranged by size:
806 517 467 321 80 80 77 61
55 49 19 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2315 2556
Size: 2315 241
EcoO109I rG'GnC_Cy
Cuts at: 0 123 952 2556
Size: 123 829 1604
Fragments arranged by size:
1604 829 123
EcoRI G'AATT_C
Cuts at: 0 733 2556
Size: 733 1823
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2050 2082 2416 2448 2556
Size: 2050 32 334 32 108
Fragments arranged by size:
2050 334 108 32 32
HincII GTy'rAC
Cuts at: 0 553 2556
Size: 553 2003
HindIII A'AGCT_T
Cuts at: 0 2233 2556
Size: 2233 323
MfeI C'AATT_G
Cuts at: 0 141 1112 2556
Size: 141 971 1444
Fragments arranged by size:
1444 971 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1358 1543 2556
Size: 797 561 185 1013
Fragments arranged by size:
1013 797 561 185
MslI CAynn'nnrTG
Cuts at: 0 2413 2556
Size: 2413 143
MspA1I CmG'CkG
Cuts at: 0 561 1075 2227 2556
Size: 561 514 1152 329
Fragments arranged by size:
1152 561 514 329
NdeI CA'TA_TG
Cuts at: 0 2301 2556
Size: 2301 255
NspI r_CATG'y
Cuts at: 0 457 780 2556
Size: 457 323 1776
Fragments arranged by size:
1776 457 323
PflMI CCAn_nnn'nTGG
Cuts at: 0 1085 2556
Size: 1085 1471
PpuMI rG'GwC_Cy
Cuts at: 0 123 2556
Size: 123 2433
PshAI GACnn'nnGTC
Cuts at: 0 1019 2556
Size: 1019 1537
PsiI TTA'TAA
Cuts at: 0 533 986 2089 2556
Size: 533 453 1103 467
Fragments arranged by size:
1103 533 467 453
PspOMI G'GGCC_C
Cuts at: 0 611 2556
Size: 611 1945
PvuII CAG'CTG
Cuts at: 0 561 1075 2227 2556
Size: 561 514 1152 329
Fragments arranged by size:
1152 561 514 329
SapI GCTCTTCn'nnn_
Cuts at: 0 1067 2165 2556
Size: 1067 1098 391
Fragments arranged by size:
1098 1067 391
SfcI C'TryA_G
Cuts at: 0 309 2556
Size: 309 2247
SmlI C'TyrA_G
Cuts at: 0 390 1017 2556
Size: 390 627 1539
Fragments arranged by size:
1539 627 390
SpeI A'CTAG_T
Cuts at: 0 2499 2556
Size: 2499 57
SspI AAT'ATT
Cuts at: 0 108 276 1714 2556
Size: 108 168 1438 842
Fragments arranged by size:
1438 842 168 108
StyI C'CwwG_G
Cuts at: 0 711 1964 2556
Size: 711 1253 592
Fragments arranged by size:
1253 711 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 17 2556
Size: 17 2539
TatI w'GTAC_w
Cuts at: 0 672 1824 2556
Size: 672 1152 732
Fragments arranged by size:
1152 732 672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2183 2215 2556
Size: 2183 32 341
Fragments arranged by size:
2183 341 32
XmnI GAAnn'nnTTC
Cuts at: 0 1394 2556
Size: 1394 1162
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AloI ApaI ApaLI
ApoI AvaI BamHI BanII BclI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaBI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI
BstEII BstYI BstZ17I BtsI CspCI DraI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
MfeI MmeI MslI MspA1I NdeI NspI PflMI PpuMI
PshAI PsiI PspOMI PvuII SapI SfcI SmlI SpeI
SspI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AlwNI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BlpI BmtI
BplI Bpu10I BsaI BsaAI BsaHI BseYI BsiEI BsiWI
BspEI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI