Restriction map for
pFN21AE2441
(Linear) (Six-base) MAPSORT of: KIEE2441.seq Check: 180 from: 1 to: 5508
KIEE2441.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 333 3705 5231 5508
Size: 333 3372 1526 277
Fragments arranged by size:
3372 1526 333 277
AccI GT'mk_AC
Cuts at: 0 548 5508
Size: 548 4960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 576 727 1158 1714 1900 2397 2680
Size: 333 243 151 431 556 186 497 283
Cuts at: 2680 3504 5508
Size: 824 2004
Fragments arranged by size:
2004 824 556 497 431 333 283 243
186 151
AfeI AGC'GCT
Cuts at: 0 3001 5233 5508
Size: 3001 2232 275
Fragments arranged by size:
3001 2232 275
AflIII A'CryG_T
Cuts at: 0 893 965 3809 4772 5255 5508
Size: 893 72 2844 963 483 253
Fragments arranged by size:
2844 963 893 483 253 72
AleI CACnn'nnGTG
Cuts at: 0 25 1638 5508
Size: 25 1613 3870
Fragments arranged by size:
3870 1613 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 5508
Size: 43 5465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5095 5127 5508
Size: 5095 32 381
Fragments arranged by size:
5095 381 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1992 2839 3068 5443 5508
Size: 1992 847 229 2375 65
Fragments arranged by size:
2375 1992 847 229 65
ApaI G_GGCC'C
Cuts at: 0 40 678 726 3399 4649 5508
Size: 40 638 48 2673 1250 859
Fragments arranged by size:
2673 1250 859 638 48 40
ApaLI G'TGCA_C
Cuts at: 0 18 5508
Size: 18 5490
AvaI C'yCGr_G
Cuts at: 0 62 533 1557 2572 2623 2647 2719 2917
Size: 62 471 1024 1015 51 24 72 198
Cuts at: 2917 2953 3767 4677 5508
Size: 36 814 910 831
Fragments arranged by size:
1024 1015 910 831 814 471 198 72
62 51 36 24
AvrII C'CTAG_G
Cuts at: 0 3459 5508
Size: 3459 2049
BamHI G'GATC_C
Cuts at: 0 249 5508
Size: 249 5259
BanI G'GyrC_C
Cuts at: 0 27 2769 3463 4681 5004 5219 5508
Size: 27 2742 694 1218 323 215 289
Fragments arranged by size:
2742 1218 694 323 289 215 27
BanII G_rGCy'C
Cuts at: 0 40 149 346 678 726 1060 1303 1557
Size: 40 109 197 332 48 334 243 254
Cuts at: 1557 2410 2551 3141 3399 4622 4649 4832 5150
Size: 853 141 590 258 1223 27 183 318
Cuts at: 5150 5508
Size: 358
Fragments arranged by size:
1223 853 590 358 334 332 318 258
254 243 197 183 141 109 48 40
27
BbeI G_GCGC'C
Cuts at: 0 3467 5508
Size: 3467 2041
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 715 765 1839 2019 2253 2334 3105
Size: 277 438 50 1074 180 234 81 771
Cuts at: 3105 3204 5508
Size: 99 2304
Fragments arranged by size:
2304 1074 771 438 277 234 180 99
81 50
BbvCI CC'TCA_GC
Cuts at: 0 1954 4951 5508
Size: 1954 2997 557
Fragments arranged by size:
2997 1954 557
BclI T'GATC_A
Cuts at: 0 413 5508
Size: 413 5095
BfrBI ATG'CAT
Cuts at: 0 2232 5508
Size: 2232 3276
BglI GCCn_nnn'nGGC
Cuts at: 0 1189 1336 1372 2223 2596 5276 5508
Size: 1189 147 36 851 373 2680 232
Fragments arranged by size:
2680 1189 851 373 232 147 36
BlpI GC'TnA_GC
Cuts at: 0 340 2404 2566 3127 5508
Size: 340 2064 162 561 2381
Fragments arranged by size:
2381 2064 561 340 162
Bme1580I G_kGCm'C
Cuts at: 0 22 40 678 726 1103 1633 2774 3399
Size: 22 18 638 48 377 530 1141 625
Cuts at: 3399 3417 4649 4684 5508
Size: 18 1232 35 824
Fragments arranged by size:
1232 1141 824 638 625 530 377 48
35 22 18 18
BmgBI CAC'GTC
Cuts at: 0 4601 5508
Size: 4601 907
BmrI ACTGGGnnnn_n'
Cuts at: 0 4890 5508
Size: 4890 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1072 1465 2621 2818 4690 4813 5508
Size: 259 813 393 1156 197 1872 123 695
Fragments arranged by size:
1872 1156 813 695 393 259 197 123
Bpu10I CC'TnA_GC
Cuts at: 0 781 1954 2545 4951 5508
Size: 781 1173 591 2406 557
Fragments arranged by size:
2406 1173 781 591 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 4123 4189 5074 5149 5508
Size: 1435 2688 66 885 75 359
Fragments arranged by size:
2688 1435 885 359 75 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 2284 3094 4552 5508
Size: 200 2084 810 1458 956
Fragments arranged by size:
2084 1458 956 810 200
BsaAI yAC'GTr
Cuts at: 0 878 896 968 3018 5508
Size: 878 18 72 2050 2490
Fragments arranged by size:
2490 2050 878 72 18
BsaBI GATnn'nnATC
Cuts at: 0 418 2473 3742 4084 4174 5425 5508
Size: 418 2055 1269 342 90 1251 83
Fragments arranged by size:
2055 1269 1251 418 342 90 83
BsaHI Gr'CG_yC
Cuts at: 0 1016 1595 3464 3875 4889 5084 5291 5399
Size: 1016 579 1869 411 1014 195 207 108
Cuts at: 5399 5508
Size: 109
Fragments arranged by size:
1869 1016 1014 579 411 207 195 109
108
BsaWI w'CCGG_w
Cuts at: 0 4403 5508
Size: 4403 1105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2173 2203 5095 5125 5508
Size: 2173 30 2892 30 383
Fragments arranged by size:
2892 2173 383 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 118 157 1408 1411 1453 2665 2800
Size: 115 3 39 1251 3 42 1212 135
Cuts at: 2800 2827 2935 2947 2959 2986 3793 4450 4453
Size: 27 108 12 12 27 807 657 3
Cuts at: 4453 4612 4831 5119 5194 5392 5508
Size: 159 219 288 75 198 116
Fragments arranged by size:
1251 1212 807 657 288 219 198 159
135 116 115 108 75 42 39 27
27 12 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 986 1096 1178 1497 2097 3348 3363 4610
Size: 986 110 82 319 600 1251 15 1247
Cuts at: 4610 5508
Size: 898
Fragments arranged by size:
1251 1247 986 898 600 319 110 82
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3016 5508
Size: 3016 2492
BsiEI CG_ry'CG
Cuts at: 0 716 4607 5508
Size: 716 3891 901
Fragments arranged by size:
3891 901 716
BsiHKAI G_wGCw'C
Cuts at: 0 22 383 1667 1769 1814 1829 2410 3089
Size: 22 361 1284 102 45 15 581 679
Cuts at: 3089 4205 5508
Size: 1116 1303
Fragments arranged by size:
1303 1284 1116 679 581 361 102 45
22 15
Bsp1286I G_dGCh'C
Cuts at: 0 22 40 149 346 383 678 726 1060
Size: 22 18 109 197 37 295 48 334
Cuts at: 1060 1103 1303 1557 1633 1667 1769 1814 1829
Size: 43 200 254 76 34 102 45 15
Cuts at: 1829 2410 2551 2774 3089 3141 3399 3417 4205
Size: 581 141 223 315 52 258 18 788
Cuts at: 4205 4622 4649 4684 4832 5150 5508
Size: 417 27 35 148 318 358
Fragments arranged by size:
788 581 417 358 334 318 315 295
258 254 223 200 197 148 141 109
102 76 52 48 45 43 37 35
34 27 22 18 18 15
BspEI T'CCGG_A
Cuts at: 0 4403 5508
Size: 4403 1105
BspHI T'CATG_A
Cuts at: 0 410 440 602 1205 3116 3479 4076 4424
Size: 410 30 162 603 1911 363 597 348
Cuts at: 4424 5508
Size: 1084
Fragments arranged by size:
1911 1084 603 597 410 363 348 162
30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 344 2222 2471 3705 5231 5508
Size: 333 11 1878 249 1234 1526 277
Fragments arranged by size:
1878 1526 1234 333 277 249 11
BsrDI GCAATG_nn'
Cuts at: 0 894 912 930 948 1524 5508
Size: 894 18 18 18 576 3984
Fragments arranged by size:
3984 894 576 18 18 18
BsrFI r'CCGG_y
Cuts at: 0 2317 3547 4606 5216 5508
Size: 2317 1230 1059 610 292
Fragments arranged by size:
2317 1230 1059 610 292
BsrGI T'GTAC_A
Cuts at: 0 4154 4775 5508
Size: 4154 621 733
Fragments arranged by size:
4154 733 621
BssSI C'ACGA_G
Cuts at: 0 4045 5176 5344 5508
Size: 4045 1131 168 164
Fragments arranged by size:
4045 1131 168 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1514 2273 3068 4580 5478 5508
Size: 1514 759 795 1512 898 30
Fragments arranged by size:
1514 1512 898 795 759 30
BstEII G'GTnAC_C
Cuts at: 0 2101 4101 5508
Size: 2101 2000 1407
Fragments arranged by size:
2101 2000 1407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 1640 3037 3348 4113 4791 5350 5508
Size: 477 1163 1397 311 765 678 559 158
Fragments arranged by size:
1397 1163 765 678 559 477 311 158
BstYI r'GATC_y
Cuts at: 0 249 497 2540 4022 5018 5198 5508
Size: 249 248 2043 1482 996 180 310
Fragments arranged by size:
2043 1482 996 310 249 248 180
Bsu36I CC'TnA_GG
Cuts at: 0 652 826 1109 2840 2930 2969 3409 5508
Size: 652 174 283 1731 90 39 440 2099
Fragments arranged by size:
2099 1731 652 440 283 174 90 39
BtgI C'CryG_G
Cuts at: 0 1331 1340 1503 1598 1636 1687 1787 1892
Size: 1331 9 163 95 38 51 100 105
Cuts at: 1892 2144 3036 3758 4386 5000 5099 5508
Size: 252 892 722 628 614 99 409
Fragments arranged by size:
1331 892 722 628 614 409 252 163
105 100 99 95 51 38 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1520 2760 4764 4887 5508
Size: 1520 1240 2004 123 621
Fragments arranged by size:
2004 1520 1240 621 123
BtsI GCAGTG_nn'
Cuts at: 0 1095 1101 3138 3388 5022 5441 5508
Size: 1095 6 2037 250 1634 419 67
Fragments arranged by size:
2037 1634 1095 419 250 67 6
ClaI AT'CG_AT
Cuts at: 0 4538 5508
Size: 4538 970
DraIII CAC_nnn'GTG
Cuts at: 0 166 878 1662 5508
Size: 166 712 784 3846
Fragments arranged by size:
3846 784 712 166
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 4264 5508
Size: 595 3669 1244
Fragments arranged by size:
3669 1244 595
EaeI y'GGCC_r
Cuts at: 0 2 1328 1337 1373 1784 1976 2036 2047
Size: 2 1326 9 36 411 192 60 11
Cuts at: 2047 2315 2319 3338 3719 4604 4808 5340 5508
Size: 268 4 1019 381 885 204 532 168
Fragments arranged by size:
1326 1019 885 532 411 381 268 204
192 168 60 36 11 9 4 2
EagI C'GGCC_G
Cuts at: 0 4604 5508
Size: 4604 904
EarI CTCTTCn'nnn_
Cuts at: 0 817 1201 1237 1607 1783 3871 4450 4471
Size: 817 384 36 370 176 2088 579 21
Cuts at: 4471 5508
Size: 1037
Fragments arranged by size:
2088 1037 817 579 384 370 176 36
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 306 1152 1189 4339 5508
Size: 306 846 37 3150 1169
Fragments arranged by size:
3150 1169 846 306 37
EcoICRI GAG'CTC
Cuts at: 0 2408 5508
Size: 2408 3100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 333 576 727 1072 1158 1465 1714
Size: 259 74 243 151 345 86 307 249
Cuts at: 1714 1900 2397 2621 2680 2818 3504 4690 4813
Size: 186 497 224 59 138 686 1186 123
Cuts at: 4813 5508
Size: 695
Fragments arranged by size:
1186 695 686 497 345 307 259 249
243 224 186 151 138 123 86 74
59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1375 2575 2662 3585 3712 5465 5508
Size: 1375 1200 87 923 127 1753 43
Fragments arranged by size:
1753 1375 1200 923 127 87 43
EcoO109I rG'GnC_Cy
Cuts at: 0 37 243 570 656 675 722 723 1494
Size: 37 206 327 86 19 47 1 771
Cuts at: 1494 2655 2925 3065 3367 3396 4646 5058 5207
Size: 1161 270 140 302 29 1250 412 149
Cuts at: 5207 5508
Size: 301
Fragments arranged by size:
1250 1161 771 412 327 302 301 270
206 149 140 86 47 37 29 19
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 321 2217 3291 4077 5508
Size: 291 30 1896 1074 786 1431
Fragments arranged by size:
1896 1431 1074 786 291 30
FseI GG_CCGG'CC
Cuts at: 0 2321 5508
Size: 2321 3187
FspI TGC'GCA
Cuts at: 0 3061 5508
Size: 3061 2447
HaeII r_GCGC'y
Cuts at: 0 2098 2420 3003 3467 5235 5508
Size: 2098 322 583 464 1768 273
Fragments arranged by size:
2098 1768 583 464 322 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 794 826 2815 2847 2866 2898 5508
Size: 794 32 1989 32 19 32 2610
Fragments arranged by size:
2610 1989 794 32 32 32 19
HincII GTy'rAC
Cuts at: 0 549 3537 3609 3675 4770 5508
Size: 549 2988 72 66 1095 738
Fragments arranged by size:
2988 1095 738 549 72 66
HindIII A'AGCT_T
Cuts at: 0 3973 5508
Size: 3973 1535
KasI G'GCGC_C
Cuts at: 0 3463 5508
Size: 3463 2045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 1085 1616 1903 2816 3715 4529 5508
Size: 731 354 531 287 913 899 814 979
Fragments arranged by size:
979 913 899 814 731 531 354 287
MscI TGG'CCA
Cuts at: 0 4 1339 1786 1978 2038 2049 3340 3721
Size: 4 1335 447 192 60 11 1291 381
Cuts at: 3721 4810 5342 5508
Size: 1089 532 166
Fragments arranged by size:
1335 1291 1089 532 447 381 192 166
60 11 4
MslI CAynn'nnrTG
Cuts at: 0 15 25 376 445 604 1638 1786 2231
Size: 15 10 351 69 159 1034 148 445
Cuts at: 2231 2311 2353 2767 3235 3439 3484 3931 4075
Size: 80 42 414 468 204 45 447 144
Cuts at: 4075 4108 4111 4165 5348 5508
Size: 33 3 54 1183 160
Fragments arranged by size:
1183 1034 468 447 445 414 351 204
160 159 148 144 80 69 54 45
42 33 15 10 3
MspA1I CmG'CkG
Cuts at: 0 90 120 1178 1239 1255 1574 1583 2160
Size: 90 30 1058 61 16 319 9 577
Cuts at: 2160 2902 3171 3656 4388 4643 4950 5282 5508
Size: 742 269 485 732 255 307 332 226
Fragments arranged by size:
1058 742 732 577 485 332 319 307
269 255 226 90 61 30 16 9
NaeI GCC'GGC
Cuts at: 0 2319 3549 4608 5218 5508
Size: 2319 1230 1059 610 290
Fragments arranged by size:
2319 1230 1059 610 290
NarI GG'CG_CC
Cuts at: 0 3464 5508
Size: 3464 2044
NcoI C'CATG_G
Cuts at: 0 1340 1503 1598 1636 1787 1892 5000 5099
Size: 1340 163 95 38 151 105 3108 99
Cuts at: 5099 5508
Size: 409
Fragments arranged by size:
3108 1340 409 163 151 105 99 95
38
NdeI CA'TA_TG
Cuts at: 0 1347 1669 5508
Size: 1347 322 3839
Fragments arranged by size:
3839 1347 322
NgoMIV G'CCGG_C
Cuts at: 0 2317 3547 4606 5216 5508
Size: 2317 1230 1059 610 292
Fragments arranged by size:
2317 1230 1059 610 292
NsiI A_TGCA'T
Cuts at: 0 2234 5508
Size: 2234 3274
NspI r_CATG'y
Cuts at: 0 405 2064 2157 2232 3813 4503 4776 5259
Size: 405 1659 93 75 1581 690 273 483
Cuts at: 5259 5508
Size: 249
Fragments arranged by size:
1659 1581 690 483 405 273 249 93
75
PasI CC'CwG_GG
Cuts at: 0 700 3030 5467 5508
Size: 700 2330 2437 41
Fragments arranged by size:
2437 2330 700 41
PciI A'CATG_T
Cuts at: 0 3809 4772 5255 5508
Size: 3809 963 483 253
Fragments arranged by size:
3809 963 483 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 1470 1504 2045 3287 4032 5028 5508
Size: 1470 34 541 1242 745 996 480
Fragments arranged by size:
1470 1242 996 745 541 480 34
PfoI T'CCnGG_A
Cuts at: 0 566 1049 4790 5508
Size: 566 483 3741 718
Fragments arranged by size:
3741 718 566 483
PmlI CAC'GTG
Cuts at: 0 878 896 968 5508
Size: 878 18 72 4540
Fragments arranged by size:
4540 878 72 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5095 5127 5347 5379 5508
Size: 5095 32 220 32 129
Fragments arranged by size:
5095 220 129 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 570 656 1494 2655 2925 3367 5058
Size: 243 327 86 838 1161 270 442 1691
Cuts at: 5058 5508
Size: 450
Fragments arranged by size:
1691 1161 838 450 442 327 270 243
86
PspOMI G'GGCC_C
Cuts at: 0 36 674 722 3395 4645 5508
Size: 36 638 48 2673 1250 863
Fragments arranged by size:
2673 1250 863 638 48 36
PspXI vC'TCGA_Gb
Cuts at: 0 2719 5508
Size: 2719 2789
PstI C_TGCA'G
Cuts at: 0 119 809 2468 2896 3716 4738 4943 5508
Size: 119 690 1659 428 820 1022 205 565
Fragments arranged by size:
1659 1022 820 690 565 428 205 119
PvuI CG_AT'CG
Cuts at: 0 716 5508
Size: 716 4792
PvuII CAG'CTG
Cuts at: 0 1178 1239 1255 2160 2902 5508
Size: 1178 61 16 905 742 2606
Fragments arranged by size:
2606 1178 905 742 61 16
SacI G_AGCT'C
Cuts at: 0 2410 5508
Size: 2410 3098
SacII CC_GC'GG
Cuts at: 0 4389 5508
Size: 4389 1119
SalI G'TCGA_C
Cuts at: 0 547 5508
Size: 547 4961
SanDI GG'GwC_CC
Cuts at: 0 570 1494 3367 5508
Size: 570 924 1873 2141
Fragments arranged by size:
2141 1873 924 570
SapI GCTCTTCn'nnn_
Cuts at: 0 817 5508
Size: 817 4691
SbfI CC_TGCA'GG
Cuts at: 0 3716 4943 5508
Size: 3716 1227 565
Fragments arranged by size:
3716 1227 565
ScaI AGT'ACT
Cuts at: 0 4228 4318 5508
Size: 4228 90 1190
Fragments arranged by size:
4228 1190 90
SexAI A'CCwGG_T
Cuts at: 0 1841 5508
Size: 1841 3667
SfcI C'TryA_G
Cuts at: 0 115 258 805 2464 2892 3712 4734 4908
Size: 115 143 547 1659 428 820 1022 174
Cuts at: 4908 4939 5227 5508
Size: 31 288 281
Fragments arranged by size:
1659 1022 820 547 428 288 281 174
143 115 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1336 1372 5508
Size: 1336 36 4136
Fragments arranged by size:
4136 1336 36
SfoI GGC'GCC
Cuts at: 0 3465 5508
Size: 3465 2043
SmaI CCC'GGG
Cuts at: 0 64 3769 4679 5508
Size: 64 3705 910 829
Fragments arranged by size:
3705 910 829 64
SmlI C'TyrA_G
Cuts at: 0 1414 2719 4138 4204 5089 5164 5508
Size: 1414 1305 1419 66 885 75 344
Fragments arranged by size:
1419 1414 1305 885 344 75 66
SphI G_CATG'C
Cuts at: 0 2157 5508
Size: 2157 3351
StuI AGG'CCT
Cuts at: 0 1973 2512 2989 4060 5508
Size: 1973 539 477 1071 1448
Fragments arranged by size:
1973 1448 1071 539 477
StyI C'CwwG_G
Cuts at: 0 1340 1454 1503 1598 1636 1787 1892 2201
Size: 1340 114 49 95 38 151 105 309
Cuts at: 2201 2507 2552 3459 4394 4833 5000 5099 5132
Size: 306 45 907 935 439 167 99 33
Cuts at: 5132 5508
Size: 376
Fragments arranged by size:
1340 935 907 439 376 309 306 167
151 114 105 99 95 49 45 38
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3240 3520 4344 4909 5053 5508
Size: 3240 280 824 565 144 455
Fragments arranged by size:
3240 824 565 455 280 144
TatI w'GTAC_w
Cuts at: 0 482 3272 4154 4226 4316 4775 5508
Size: 482 2790 882 72 90 459 733
Fragments arranged by size:
2790 882 733 482 459 90 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1074 1106 1982 2014 2173 2205 4353 4385
Size: 1074 32 876 32 159 32 2148 32
Cuts at: 4385 5508
Size: 1123
Fragments arranged by size:
2148 1123 1074 876 159 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3208 5508
Size: 3208 2300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1605 2721 3330 5058 5508
Size: 1605 1116 609 1728 450
Fragments arranged by size:
1728 1605 1116 609 450
XhoI C'TCGA_G
Cuts at: 0 2719 5508
Size: 2719 2789
XmaI C'CCGG_G
Cuts at: 0 62 3767 4677 5508
Size: 62 3705 910 831
Fragments arranged by size:
3705 910 831 62
XmnI GAAnn'nnTTC
Cuts at: 0 274 1231 1930 2386 4318 5508
Size: 274 957 699 456 1932 1190
Fragments arranged by size:
1932 1190 957 699 456 274
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI ApoI AscI
AseI AsiSI BaeI BcgI BciVI BglII BmtI BplI
BsiWI BsmI BsmBI BsrBI BssHII BstBI BstZ17I CspCI
DraI EcoRI EcoRV FspAI HpaI KpnI MfeI MluI
NheI NotI NruI PacI PmeI PshAI PsiI PsrI
RsrII SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI ZraI