Restriction map for
pFN21AE2425
(Linear) (Six-base) MAPSORT of: KIEE2425.seq Check: 8621 from: 1 to: 987
KIEE2425.orf
With 182 enzymes: SgfI *
November 22, 2012 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 604 987
Size: 604 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 457 987
Size: 303 154 530
Fragments arranged by size:
530 303 154
AleI CACnn'nnGTG
Cuts at: 0 249 987
Size: 249 738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 987
Size: 316 671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 987
Size: 517 32 438
Fragments arranged by size:
517 438 32
ApaI G_GGCC'C
Cuts at: 0 91 388 468 583 987
Size: 91 297 80 115 404
Fragments arranged by size:
404 297 115 91 80
AvaI C'yCGr_G
Cuts at: 0 61 91 987
Size: 61 30 896
Fragments arranged by size:
896 61 30
BanI G'GyrC_C
Cuts at: 0 721 987
Size: 721 266
BanII G_rGCy'C
Cuts at: 0 91 388 468 583 987
Size: 91 297 80 115 404
Fragments arranged by size:
404 297 115 91 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 987
Size: 708 279
BbvCI CC'TCA_GC
Cuts at: 0 127 987
Size: 127 860
BglI GCCn_nnn'nGGC
Cuts at: 0 54 987
Size: 54 933
Bme1580I G_kGCm'C
Cuts at: 0 91 388 468 583 987
Size: 91 297 80 115 404
Fragments arranged by size:
404 297 115 91 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 346 880 987
Size: 285 61 534 107
Fragments arranged by size:
534 285 107 61
Bpu10I CC'TnA_GC
Cuts at: 0 127 987
Size: 127 860
BsaAI yAC'GTr
Cuts at: 0 147 987
Size: 147 840
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 987
Size: 517 30 440
Fragments arranged by size:
517 440 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 987
Size: 538 449
BseYI C'CCAG_C
Cuts at: 0 9 75 104 339 807 987
Size: 9 66 29 235 468 180
Fragments arranged by size:
468 235 180 66 29 9
BsiEI CG_ry'CG
Cuts at: 0 49 58 987
Size: 49 9 929
Fragments arranged by size:
929 49 9
BsiHKAI G_wGCw'C
Cuts at: 0 833 985 987
Size: 833 152 2
Fragments arranged by size:
833 152 2
Bsp1286I G_dGCh'C
Cuts at: 0 91 388 468 583 833 985 987
Size: 91 297 80 115 250 152 2
Fragments arranged by size:
297 250 152 115 91 80 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 604 987
Size: 604 383
BsrBI CCG'CTC
Cuts at: 0 54 458 603 954 987
Size: 54 404 145 351 33
Fragments arranged by size:
404 351 145 54 33
BsrFI r'CCGG_y
Cuts at: 0 768 987
Size: 768 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 862 987
Size: 862 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 987
Size: 225 762
BtgI C'CryG_G
Cuts at: 0 194 987
Size: 194 793
BtsI GCAGTG_nn'
Cuts at: 0 728 987
Size: 728 259
EaeI y'GGCC_r
Cuts at: 0 46 55 987
Size: 46 9 932
Fragments arranged by size:
932 46 9
EagI C'GGCC_G
Cuts at: 0 46 55 987
Size: 46 9 932
Fragments arranged by size:
932 46 9
EarI CTCTTCn'nnn_
Cuts at: 0 163 745 886 987
Size: 163 582 141 101
Fragments arranged by size:
582 163 141 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 643 987
Size: 643 344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 303 346 457 880 987
Size: 285 18 43 111 423 107
Fragments arranged by size:
423 285 111 107 43 18
EcoO109I rG'GnC_Cy
Cuts at: 0 88 580 987
Size: 88 492 407
Fragments arranged by size:
492 407 88
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 987
Size: 228 759
HaeII r_GCGC'y
Cuts at: 0 10 76 349 987
Size: 10 66 273 638
Fragments arranged by size:
638 273 66 10
MslI CAynn'nnrTG
Cuts at: 0 249 310 481 987
Size: 249 61 171 506
Fragments arranged by size:
506 249 171 61
MspA1I CmG'CkG
Cuts at: 0 196 343 470 516 645 712 811 987
Size: 196 147 127 46 129 67 99 176
Fragments arranged by size:
196 176 147 129 127 99 67 46
NotI GC'GGCC_GC
Cuts at: 0 55 987
Size: 55 932
NspI r_CATG'y
Cuts at: 0 486 987
Size: 486 501
PasI CC'CwG_GG
Cuts at: 0 182 867 987
Size: 182 685 120
Fragments arranged by size:
685 182 120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 987
Size: 517 32 438
Fragments arranged by size:
517 438 32
PshAI GACnn'nnGTC
Cuts at: 0 33 987
Size: 33 954
PspOMI G'GGCC_C
Cuts at: 0 87 384 464 579 987
Size: 87 297 80 115 408
Fragments arranged by size:
408 297 115 87 80
PvuII CAG'CTG
Cuts at: 0 645 712 811 987
Size: 645 67 99 176
Fragments arranged by size:
645 176 99 67
SacII CC_GC'GG
Cuts at: 0 197 987
Size: 197 790
SapI GCTCTTCn'nnn_
Cuts at: 0 163 987
Size: 163 824
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 54 987
Size: 54 933
SmaI CCC'GGG
Cuts at: 0 93 987
Size: 93 894
StyI C'CwwG_G
Cuts at: 0 824 987
Size: 824 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 96 848 859 987
Size: 96 752 11 128
Fragments arranged by size:
752 128 96 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 987
Size: 225 762
XmaI C'CCGG_G
Cuts at: 0 91 987
Size: 91 896
XmnI GAAnn'nnTTC
Cuts at: 0 676 987
Size: 676 311
Enzymes that do cut:
AarI AcuI AleI AlfI AloI ApaI AvaI BanI
BanII BbsI BbvCI BglI Bme1580I BpmI Bpu10I BsaAI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstXI BtgI BtsI EaeI EagI EarI
EciI Eco57MI EcoO109I FalI HaeII MslI MspA1I NotI
NspI PasI PpiI PshAI PspOMI PvuII SacII SapI
SfiI SmaI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpuMI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI ZraI