Restriction map for
pFN21AE2417
(Linear) (Six-base) MAPSORT of: KIEE2417.seq Check: 4331 from: 1 to: 1341
KIEE2417.orf
With 182 enzymes: SgfI *
July 23, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 486 1341
Size: 486 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1088 1341
Size: 415 673 253
Fragments arranged by size:
673 415 253
AleI CACnn'nnGTG
Cuts at: 0 1213 1341
Size: 1213 128
AlwNI CAG_nnn'CTG
Cuts at: 0 931 1144 1147 1341
Size: 931 213 3 194
Fragments arranged by size:
931 213 194 3
ApaLI G'TGCA_C
Cuts at: 0 965 1341
Size: 965 376
ApoI r'AATT_y
Cuts at: 0 913 1341
Size: 913 428
AvaI C'yCGr_G
Cuts at: 0 1303 1341
Size: 1303 38
BanI G'GyrC_C
Cuts at: 0 501 1341
Size: 501 840
BanII G_rGCy'C
Cuts at: 0 533 1058 1102 1304 1340 1341
Size: 533 525 44 202 36 1
Fragments arranged by size:
533 525 202 44 36 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 815 1341
Size: 815 526
BbvCI CC'TCA_GC
Cuts at: 0 92 1341
Size: 92 1249
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 1341
Size: 1236 105
BfrBI ATG'CAT
Cuts at: 0 1314 1341
Size: 1314 27
Bme1580I G_kGCm'C
Cuts at: 0 969 1341
Size: 969 372
BmrI ACTGGGnnnn_n'
Cuts at: 0 919 1341
Size: 919 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1341
Size: 364 977
Bpu10I CC'TnA_GC
Cuts at: 0 92 527 1341
Size: 92 435 814
Fragments arranged by size:
814 435 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1341
Size: 151 1190
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 1341
Size: 238 1103
BsaAI yAC'GTr
Cuts at: 0 729 1341
Size: 729 612
BsaBI GATnn'nnATC
Cuts at: 0 46 1341
Size: 46 1295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 362 392 1341
Size: 362 30 949
Fragments arranged by size:
949 362 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 1341
Size: 226 1115
BseYI C'CCAG_C
Cuts at: 0 885 1014 1126 1137 1341
Size: 885 129 112 11 204
Fragments arranged by size:
885 204 129 112 11
BsiHKAI G_wGCw'C
Cuts at: 0 533 969 1341
Size: 533 436 372
Fragments arranged by size:
533 436 372
BsmI GAATG_Cn'
Cuts at: 0 759 878 1341
Size: 759 119 463
Fragments arranged by size:
759 463 119
Bsp1286I G_dGCh'C
Cuts at: 0 533 969 1058 1102 1304 1340 1341
Size: 533 436 89 44 202 36 1
Fragments arranged by size:
533 436 202 89 44 36 1
BspHI T'CATG_A
Cuts at: 0 776 1341
Size: 776 565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 486 1047 1341
Size: 486 561 294
Fragments arranged by size:
561 486 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1215 1341
Size: 1215 126
Bsu36I CC'TnA_GG
Cuts at: 0 1070 1341
Size: 1070 271
BtgI C'CryG_G
Cuts at: 0 259 1214 1238 1341
Size: 259 955 24 103
Fragments arranged by size:
955 259 103 24
BtsI GCAGTG_nn'
Cuts at: 0 83 1341
Size: 83 1258
EarI CTCTTCn'nnn_
Cuts at: 0 260 1341
Size: 260 1081
EcoICRI GAG'CTC
Cuts at: 0 531 1341
Size: 531 810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 415 1088 1341
Size: 364 51 673 253
Fragments arranged by size:
673 364 253 51
EcoO109I rG'GnC_Cy
Cuts at: 0 36 943 1187 1341
Size: 36 907 244 154
Fragments arranged by size:
907 244 154 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 445 1118 1341
Size: 445 673 223
Fragments arranged by size:
673 445 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 758 790 1341
Size: 216 32 510 32 551
Fragments arranged by size:
551 510 216 32 32
MfeI C'AATT_G
Cuts at: 0 748 1341
Size: 748 593
MslI CAynn'nnrTG
Cuts at: 0 1213 1341
Size: 1213 128
MspA1I CmG'CkG
Cuts at: 0 1144 1341
Size: 1144 197
NcoI C'CATG_G
Cuts at: 0 259 1238 1341
Size: 259 979 103
Fragments arranged by size:
979 259 103
NsiI A_TGCA'T
Cuts at: 0 1316 1341
Size: 1316 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 844 1341
Size: 844 497
PfoI T'CCnGG_A
Cuts at: 0 59 714 837 1223 1341
Size: 59 655 123 386 118
Fragments arranged by size:
655 386 123 118 59
PpuMI rG'GwC_Cy
Cuts at: 0 36 1187 1341
Size: 36 1151 154
Fragments arranged by size:
1151 154 36
PstI C_TGCA'G
Cuts at: 0 161 1341
Size: 161 1180
PvuII CAG'CTG
Cuts at: 0 1144 1341
Size: 1144 197
SacI G_AGCT'C
Cuts at: 0 533 1341
Size: 533 808
SfcI C'TryA_G
Cuts at: 0 157 1341
Size: 157 1184
SmlI C'TyrA_G
Cuts at: 0 130 1341
Size: 130 1211
SspI AAT'ATT
Cuts at: 0 301 1341
Size: 301 1040
StyI C'CwwG_G
Cuts at: 0 259 1093 1238 1341
Size: 259 834 145 103
Fragments arranged by size:
834 259 145 103
XbaI T'CTAG_A
Cuts at: 0 187 1005 1341
Size: 187 818 336
Fragments arranged by size:
818 336 187
XmnI GAAnn'nnTTC
Cuts at: 0 428 1341
Size: 428 913
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BfrBI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BstXI Bsu36I BtgI
BtsI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MfeI
MslI MspA1I NcoI NsiI PflMI PfoI PpuMI PstI
PvuII SacI SfcI SmlI SspI StyI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BglI BglII BlpI
BmgBI BmtI BplI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI TstI Tth111I
XcmI XhoI XmaI ZraI