(Linear) (Six-base) MAPSORT of: KIEE2415.seq  Check: 9708  from: 1  to: 1377



KIEE2415.orf

 With 182 enzymes: SgfI * 

                        July 10, 2012 13:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     444    1377 
   Size:        444     933
 
AccI GT'mk_AC

Cuts at:      0     305    1325    1377 
   Size:        305    1020      52

  Fragments arranged by size: 

                1020     305      52
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29    1377 
   Size:         29    1348
 
AflIII A'CryG_T

Cuts at:      0    1259    1377 
   Size:       1259     118
 
AleI CACnn'nnGTG

Cuts at:      0     925    1195    1377 
   Size:        925     270     182

  Fragments arranged by size: 

                 925     270     182
 
ApoI r'AATT_y

Cuts at:      0      39    1377 
   Size:         39    1338
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     793     826    1377 
   Size:        793      33     551

  Fragments arranged by size: 

                 793     551      33
 
BamHI G'GATC_C

Cuts at:      0    1343    1377 
   Size:       1343      34
 
BanI G'GyrC_C

Cuts at:      0     372    1377 
   Size:        372    1005
 
BanII G_rGCy'C

Cuts at:      0     384     423    1377 
   Size:        384      39     954

  Fragments arranged by size: 

                 954     384      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     166     627    1377 
   Size:        166     461     750

  Fragments arranged by size: 

                 750     461     166
 
BbvCI CC'TCA_GC

Cuts at:      0    1020    1219    1377 
   Size:       1020     199     158

  Fragments arranged by size: 

                1020     199     158
 
BclI T'GATC_A

Cuts at:      0    1295    1377 
   Size:       1295      82
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109     170    1165    1377 
   Size:        109      61     995     212

  Fragments arranged by size: 

                 995     212     109      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     409    1146    1377 
   Size:        100     309     737     231

  Fragments arranged by size: 

                 737     309     231     100
 
Bpu10I CC'TnA_GC

Cuts at:      0    1020    1219    1377 
   Size:       1020     199     158

  Fragments arranged by size: 

                1020     199     158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     976    1377 
   Size:        213     763     401

  Fragments arranged by size: 

                 763     401     213
 
BsaAI yAC'GTr

Cuts at:      0     387    1260    1377 
   Size:        387     873     117

  Fragments arranged by size: 

                 873     387     117
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     371     401    1377 
   Size:        371      30     976

  Fragments arranged by size: 

                 976     371      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1008    1377 
   Size:       1008     369
 
BseYI C'CCAG_C

Cuts at:      0    1355    1377 
   Size:       1355      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1377 
   Size:        203    1174
 
BsiHKAI G_wGCw'C

Cuts at:      0     438    1377 
   Size:        438     939
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     656    1377 
   Size:        656     721
 
Bsp1286I G_dGCh'C

Cuts at:      0     384     423     438    1377 
   Size:        384      39      15     939

  Fragments arranged by size: 

                 939     384      39      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     444    1377 
   Size:        444     933
 
BsrDI GCAATG_nn'

Cuts at:      0    1119    1377 
   Size:       1119     258
 
BsrGI T'GTAC_A

Cuts at:      0    1180    1377 
   Size:       1180     197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     442    1377 
   Size:        442     935
 
BstYI r'GATC_y

Cuts at:      0    1343    1377 
   Size:       1343      34
 
BtsI GCAGTG_nn'

Cuts at:      0     757    1377 
   Size:        757     620
 
EarI CTCTTCn'nnn_

Cuts at:      0    1204    1377 
   Size:       1204     173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1336    1377 
   Size:       1336      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     100     409    1146    1377 
   Size:         29      71     309     737     231

  Fragments arranged by size: 

                 737     309     231      71      29
 
EcoRI G'AATT_C

Cuts at:      0      39    1377 
   Size:         39    1338
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     526     558     653     685    1377 
   Size:        526      32      95      32     692

  Fragments arranged by size: 

                 692     526      95      32      32
 
HincII GTy'rAC

Cuts at:      0    1122    1377 
   Size:       1122     255
 
MslI CAynn'nnrTG

Cuts at:      0     925    1195    1377 
   Size:        925     270     182

  Fragments arranged by size: 

                 925     270     182
 
NdeI CA'TA_TG

Cuts at:      0    1041    1377 
   Size:       1041     336
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1194    1377 
   Size:       1194     183
 
PmlI CAC'GTG

Cuts at:      0     387    1377 
   Size:        387     990
 
PsiI TTA'TAA

Cuts at:      0     658    1377 
   Size:        658     719
 
PstI C_TGCA'G

Cuts at:      0     584    1254    1377 
   Size:        584     670     123

  Fragments arranged by size: 

                 670     584     123
 
SfcI C'TryA_G

Cuts at:      0     580    1250    1377 
   Size:        580     670     127

  Fragments arranged by size: 

                 670     580     127
 
SmlI C'TyrA_G

Cuts at:      0     192     991    1377 
   Size:        192     799     386

  Fragments arranged by size: 

                 799     386     192
 
SspI AAT'ATT

Cuts at:      0     299    1377 
   Size:        299    1078
 
TatI w'GTAC_w

Cuts at:      0    1077    1180    1377 
   Size:       1077     103     197

  Fragments arranged by size: 

                1077     197     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     371     403    1377 
   Size:        371      32     974

  Fragments arranged by size: 

                 974     371      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1092    1213    1377 
   Size:       1092     121     164

  Fragments arranged by size: 

                1092     164     121
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     ApoI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrDI    BsrGI   BstAPI    BstYI     BtsI     EarI
     EciI  Eco57MI    EcoRI    Hin4I   HincII     MslI     NdeI    PflMI
     PmlI     PsiI     PstI     SfcI     SmlI     SspI     TatI     TstI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BbeI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI