Restriction map for
pFN21AE2410
(Linear) (Six-base) MAPSORT of: KIEE2410.seq Check: 4178 from: 1 to: 858
KIEE2410.orf
With 182 enzymes: SgfI *
May 24, 2012 13:15 ..
AleI CACnn'nnGTG
Cuts at: 0 691 858
Size: 691 167
AvaI C'yCGr_G
Cuts at: 0 23 842 858
Size: 23 819 16
Fragments arranged by size:
819 23 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 242 275 858
Size: 242 33 583
Fragments arranged by size:
583 242 33
BanI G'GyrC_C
Cuts at: 0 461 599 628 849 858
Size: 461 138 29 221 9
Fragments arranged by size:
461 221 138 29 9
BanII G_rGCy'C
Cuts at: 0 24 198 858
Size: 24 174 660
Fragments arranged by size:
660 174 24
BbvCI CC'TCA_GC
Cuts at: 0 176 858
Size: 176 682
BciVI GTATCCnnnnn_n'
Cuts at: 0 287 858
Size: 287 571
BlpI GC'TnA_GC
Cuts at: 0 71 858
Size: 71 787
Bme1580I G_kGCm'C
Cuts at: 0 604 779 852 858
Size: 604 175 73 6
Fragments arranged by size:
604 175 73 6
BmgBI CAC'GTC
Cuts at: 0 294 858
Size: 294 564
Bpu10I CC'TnA_GC
Cuts at: 0 176 200 858
Size: 176 24 658
Fragments arranged by size:
658 176 24
BsaAI yAC'GTr
Cuts at: 0 190 858
Size: 190 668
BsiHKAI G_wGCw'C
Cuts at: 0 198 858
Size: 198 660
Bsp1286I G_dGCh'C
Cuts at: 0 24 198 604 779 852 858
Size: 24 174 406 175 73 6
Fragments arranged by size:
406 175 174 73 24 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 858
Size: 171 687
BsrDI GCAATG_nn'
Cuts at: 0 65 552 858
Size: 65 487 306
Fragments arranged by size:
487 306 65
BsrFI r'CCGG_y
Cuts at: 0 355 858
Size: 355 503
BssSI C'ACGA_G
Cuts at: 0 206 858
Size: 206 652
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 774 858
Size: 468 306 84
Fragments arranged by size:
468 306 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 112 858
Size: 112 746
BtsI GCAGTG_nn'
Cuts at: 0 83 858
Size: 83 775
DraIII CAC_nnn'GTG
Cuts at: 0 468 858
Size: 468 390
EaeI y'GGCC_r
Cuts at: 0 114 507 858
Size: 114 393 351
Fragments arranged by size:
393 351 114
EarI CTCTTCn'nnn_
Cuts at: 0 491 665 858
Size: 491 174 193
Fragments arranged by size:
491 193 174
EcoICRI GAG'CTC
Cuts at: 0 196 858
Size: 196 662
EcoO109I rG'GnC_Cy
Cuts at: 0 725 858
Size: 725 133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 729 858
Size: 729 129
FspI TGC'GCA
Cuts at: 0 475 858
Size: 475 383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 816 848 858
Size: 571 32 213 32 10
Fragments arranged by size:
571 213 32 32 10
HincII GTy'rAC
Cuts at: 0 291 858
Size: 291 567
MscI TGG'CCA
Cuts at: 0 116 509 858
Size: 116 393 349
Fragments arranged by size:
393 349 116
MslI CAynn'nnrTG
Cuts at: 0 575 691 858
Size: 575 116 167
Fragments arranged by size:
575 167 116
NdeI CA'TA_TG
Cuts at: 0 690 858
Size: 690 168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 148 180 858
Size: 11 32 105 32 678
Fragments arranged by size:
678 105 32 32 11
PpuMI rG'GwC_Cy
Cuts at: 0 725 858
Size: 725 133
PspXI vC'TCGA_Gb
Cuts at: 0 842 858
Size: 842 16
SacI G_AGCT'C
Cuts at: 0 198 858
Size: 198 660
SmlI C'TyrA_G
Cuts at: 0 842 858
Size: 842 16
SnaBI TAC'GTA
Cuts at: 0 190 858
Size: 190 668
StyI C'CwwG_G
Cuts at: 0 713 858
Size: 713 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 858
Size: 264 594
TatI w'GTAC_w
Cuts at: 0 683 858
Size: 683 175
XbaI T'CTAG_A
Cuts at: 0 793 858
Size: 793 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 109 455 858
Size: 109 346 403
Fragments arranged by size:
403 346 109
XhoI C'TCGA_G
Cuts at: 0 842 858
Size: 842 16
Enzymes that do cut:
AleI AvaI BaeI BanI BanII BbvCI BciVI BlpI
Bme1580I BmgBI Bpu10I BsaAI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssSI BstAPI BstXI BtsI DraIII EaeI EarI
EcoICRI EcoO109I FalI FspI Hin4I HincII MscI MslI
NdeI PpiI PpuMI PspXI SacI SmlI SnaBI StyI
TaqII TatI XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI
BcgI BclI BfrBI BglI BglII BmrI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
Eco57MI EcoNI EcoRI EcoRV FseI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XmaI XmnI ZraI