Restriction map for
pFN21AE2405
(Linear) (Six-base) MAPSORT of: KIEE2405.seq Check: 6684 from: 1 to: 1179
KIEE2405.orf
With 182 enzymes: SgfI *
February 28, 2012 13:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 656 1052 1179
Size: 359 297 396 127
Fragments arranged by size:
396 359 297 127
AflIII A'CryG_T
Cuts at: 0 405 945 1179
Size: 405 540 234
Fragments arranged by size:
540 405 234
AleI CACnn'nnGTG
Cuts at: 0 877 1179
Size: 877 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 883 1179
Size: 883 296
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1179
Size: 150 1029
ApoI r'AATT_y
Cuts at: 0 201 505 570 1179
Size: 201 304 65 609
Fragments arranged by size:
609 304 201 65
BbvCI CC'TCA_GC
Cuts at: 0 934 1179
Size: 934 245
BciVI GTATCCnnnnn_n'
Cuts at: 0 197 1179
Size: 197 982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 710 1179
Size: 708 2 469
Fragments arranged by size:
708 469 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 703 1179
Size: 127 576 476
Fragments arranged by size:
576 476 127
Bpu10I CC'TnA_GC
Cuts at: 0 934 1179
Size: 934 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 779 1179
Size: 689 90 400
Fragments arranged by size:
689 400 90
BsaWI w'CCGG_w
Cuts at: 0 487 635 1179
Size: 487 148 544
Fragments arranged by size:
544 487 148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 1179
Size: 126 1053
BsiHKAI G_wGCw'C
Cuts at: 0 424 1179
Size: 424 755
Bsp1286I G_dGCh'C
Cuts at: 0 424 1179
Size: 424 755
BspHI T'CATG_A
Cuts at: 0 926 1179
Size: 926 253
BsrGI T'GTAC_A
Cuts at: 0 284 1125 1179
Size: 284 841 54
Fragments arranged by size:
841 284 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 562 1179
Size: 81 481 617
Fragments arranged by size:
617 481 81
BstYI r'GATC_y
Cuts at: 0 798 1179
Size: 798 381
Bsu36I CC'TnA_GG
Cuts at: 0 159 1179
Size: 159 1020
BtgI C'CryG_G
Cuts at: 0 80 1179
Size: 80 1099
DraI TTT'AAA
Cuts at: 0 179 197 1179
Size: 179 18 982
Fragments arranged by size:
982 179 18
EaeI y'GGCC_r
Cuts at: 0 230 1044 1179
Size: 230 814 135
Fragments arranged by size:
814 230 135
EarI CTCTTCn'nnn_
Cuts at: 0 656 770 907 925 1028 1179
Size: 656 114 137 18 103 151
Fragments arranged by size:
656 151 137 114 103 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 359 656 703 1052 1179
Size: 127 232 297 47 349 127
Fragments arranged by size:
349 297 232 127 127 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 1179
Size: 87 32 1060
Fragments arranged by size:
1060 87 32
HindIII A'AGCT_T
Cuts at: 0 371 1179
Size: 371 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1179
Size: 618 561
MscI TGG'CCA
Cuts at: 0 1046 1179
Size: 1046 133
MslI CAynn'nnrTG
Cuts at: 0 79 250 481 560 877 1179
Size: 79 171 231 79 317 302
Fragments arranged by size:
317 302 231 171 79 79
NspI r_CATG'y
Cuts at: 0 409 949 1179
Size: 409 540 230
Fragments arranged by size:
540 409 230
PciI A'CATG_T
Cuts at: 0 405 945 1179
Size: 405 540 234
Fragments arranged by size:
540 405 234
PfoI T'CCnGG_A
Cuts at: 0 365 1179
Size: 365 814
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 395 427 1179
Size: 395 32 752
Fragments arranged by size:
752 395 32
SapI GCTCTTCn'nnn_
Cuts at: 0 925 1179
Size: 925 254
ScaI AGT'ACT
Cuts at: 0 729 1179
Size: 729 450
SmlI C'TyrA_G
Cuts at: 0 704 794 1179
Size: 704 90 385
Fragments arranged by size:
704 385 90
SpeI A'CTAG_T
Cuts at: 0 621 1179
Size: 621 558
StuI AGG'CCT
Cuts at: 0 49 605 1179
Size: 49 556 574
Fragments arranged by size:
574 556 49
TatI w'GTAC_w
Cuts at: 0 284 411 727 754 1091 1125 1179
Size: 284 127 316 27 337 34 54
Fragments arranged by size:
337 316 284 127 54 34 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 783 815 1179
Size: 783 32 364
Fragments arranged by size:
783 364 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI BbvCI BciVI
BplI BpmI Bpu10I BpuEI BsaWI BseRI BsiHKAI Bsp1286I
BspHI BsrGI BstXI BstYI Bsu36I BtgI DraI EaeI
EarI Eco57MI Hin4I HindIII MmeI MscI MslI NspI
PciI PfoI PsrI SapI ScaI SmlI SpeI StuI
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI