Restriction map for
pFN21AE2404
(Linear) (Six-base) MAPSORT of: KIEE2404.seq Check: 3197 from: 1 to: 1083
KIEE2404.orf
With 182 enzymes: SgfI *
February 28, 2012 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 1083
Size: 24 1059
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1083
Size: 1051 32
AflII C'TTAA_G
Cuts at: 0 334 1083
Size: 334 749
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 1083
Size: 478 32 573
Fragments arranged by size:
573 478 32
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1083
Size: 150 933
ApoI r'AATT_y
Cuts at: 0 660 823 1000 1083
Size: 660 163 177 83
Fragments arranged by size:
660 177 163 83
AseI AT'TA_AT
Cuts at: 0 671 1083
Size: 671 412
BanI G'GyrC_C
Cuts at: 0 867 1083
Size: 867 216
BanII G_rGCy'C
Cuts at: 0 67 1083
Size: 67 1016
BbvCI CC'TCA_GC
Cuts at: 0 103 619 1083
Size: 103 516 464
Fragments arranged by size:
516 464 103
Bpu10I CC'TnA_GC
Cuts at: 0 103 619 1083
Size: 103 516 464
Fragments arranged by size:
516 464 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 325 1083
Size: 60 265 758
Fragments arranged by size:
758 265 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 1083
Size: 166 917
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 615 645 1083
Size: 478 30 107 30 438
Fragments arranged by size:
478 438 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 150 188 196 636 1052 1083
Size: 90 60 38 8 440 416 31
Fragments arranged by size:
440 416 90 60 38 31 8
BseYI C'CCAG_C
Cuts at: 0 28 448 1083
Size: 28 420 635
Fragments arranged by size:
635 420 28
BsiHKAI G_wGCw'C
Cuts at: 0 67 1083
Size: 67 1016
Bsp1286I G_dGCh'C
Cuts at: 0 67 1083
Size: 67 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 1083
Size: 24 1059
BsrDI GCAATG_nn'
Cuts at: 0 474 1083
Size: 474 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 505 1083
Size: 505 578
BtgI C'CryG_G
Cuts at: 0 576 1083
Size: 576 507
DrdI GACnn_nn'nnGTC
Cuts at: 0 556 1083
Size: 556 527
EaeI y'GGCC_r
Cuts at: 0 573 579 1083
Size: 573 6 504
Fragments arranged by size:
573 504 6
EarI CTCTTCn'nnn_
Cuts at: 0 182 683 1083
Size: 182 501 400
Fragments arranged by size:
501 400 182
EcoICRI GAG'CTC
Cuts at: 0 65 1083
Size: 65 1018
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1083
Size: 1051 32
EcoO109I rG'GnC_Cy
Cuts at: 0 192 954 1083
Size: 192 762 129
Fragments arranged by size:
762 192 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 1083
Size: 378 705
HaeII r_GCGC'y
Cuts at: 0 846 1083
Size: 846 237
HincII GTy'rAC
Cuts at: 0 225 1083
Size: 225 858
MscI TGG'CCA
Cuts at: 0 581 1083
Size: 581 502
MspA1I CmG'CkG
Cuts at: 0 90 150 731 1083
Size: 90 60 581 352
Fragments arranged by size:
581 352 90 60
NdeI CA'TA_TG
Cuts at: 0 1051 1083
Size: 1051 32
PfoI T'CCnGG_A
Cuts at: 0 482 971 1083
Size: 482 489 112
Fragments arranged by size:
489 482 112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1083
Size: 478 32 573
Fragments arranged by size:
573 478 32
PpuMI rG'GwC_Cy
Cuts at: 0 192 954 1083
Size: 192 762 129
Fragments arranged by size:
762 192 129
PvuII CAG'CTG
Cuts at: 0 150 1083
Size: 150 933
SacI G_AGCT'C
Cuts at: 0 67 1083
Size: 67 1016
SfcI C'TryA_G
Cuts at: 0 936 1083
Size: 936 147
SmlI C'TyrA_G
Cuts at: 0 39 304 334 1083
Size: 39 265 30 749
Fragments arranged by size:
749 265 39 30
StyI C'CwwG_G
Cuts at: 0 582 1083
Size: 582 501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 969 1083
Size: 969 114
XmnI GAAnn'nnTTC
Cuts at: 0 608 739 1083
Size: 608 131 344
Fragments arranged by size:
608 344 131
Enzymes that do cut:
AarI AcuI AflII AloI AlwNI ApoI AseI BanI
BanII BbvCI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrDI BstXI BtgI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII HincII MscI
MspA1I NdeI PfoI PpiI PpuMI PvuII SacI SfcI
SmlI StyI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI