Restriction map for
pFN21AE2384
(Linear) (Six-base) MAPSORT of: KIEE2384.seq Check: 8233 from: 1 to: 948
KIEE2384.orf
With 182 enzymes: SgfI *
November 11, 2011 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 186 948
Size: 69 117 762
Fragments arranged by size:
762 117 69
AatII G_ACGT'C
Cuts at: 0 113 948
Size: 113 835
Acc65I G'GTAC_C
Cuts at: 0 55 116 948
Size: 55 61 832
Fragments arranged by size:
832 61 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 948
Size: 319 629
AflIII A'CryG_T
Cuts at: 0 419 948
Size: 419 529
AlwNI CAG_nnn'CTG
Cuts at: 0 656 744 884 948
Size: 656 88 140 64
Fragments arranged by size:
656 140 88 64
ApaLI G'TGCA_C
Cuts at: 0 240 948
Size: 240 708
ApoI r'AATT_y
Cuts at: 0 361 948
Size: 361 587
AvaI C'yCGr_G
Cuts at: 0 667 948
Size: 667 281
BamHI G'GATC_C
Cuts at: 0 690 948
Size: 690 258
BanI G'GyrC_C
Cuts at: 0 28 55 116 948
Size: 28 27 61 832
Fragments arranged by size:
832 61 28 27
BanII G_rGCy'C
Cuts at: 0 218 948
Size: 218 730
BbeI G_GCGC'C
Cuts at: 0 32 948
Size: 32 916
BbsI GAAGACnn'nnnn_
Cuts at: 0 289 948
Size: 289 659
BglI GCCn_nnn'nGGC
Cuts at: 0 638 948
Size: 638 310
Bme1580I G_kGCm'C
Cuts at: 0 138 244 948
Size: 138 106 704
Fragments arranged by size:
704 138 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 392 948
Size: 392 556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 478 706 906 948
Size: 56 422 228 200 42
Fragments arranged by size:
422 228 200 56 42
Bpu10I CC'TnA_GC
Cuts at: 0 187 948
Size: 187 761
BsaAI yAC'GTr
Cuts at: 0 245 422 948
Size: 245 177 526
Fragments arranged by size:
526 245 177
BsaBI GATnn'nnATC
Cuts at: 0 40 948
Size: 40 908
BsaHI Gr'CG_yC
Cuts at: 0 29 110 948
Size: 29 81 838
Fragments arranged by size:
838 81 29
BsaWI w'CCGG_w
Cuts at: 0 749 948
Size: 749 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 879 909 948
Size: 879 30 39
Fragments arranged by size:
879 39 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 948
Size: 226 722
BsiHKAI G_wGCw'C
Cuts at: 0 218 244 948
Size: 218 26 704
Fragments arranged by size:
704 218 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 948
Size: 16 932
Bsp1286I G_dGCh'C
Cuts at: 0 138 218 244 948
Size: 138 80 26 704
Fragments arranged by size:
704 138 80 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 186 948
Size: 69 117 762
Fragments arranged by size:
762 117 69
BsrDI GCAATG_nn'
Cuts at: 0 393 806 948
Size: 393 413 142
Fragments arranged by size:
413 393 142
BstEII G'GTnAC_C
Cuts at: 0 618 948
Size: 618 330
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 948
Size: 414 534
BstYI r'GATC_y
Cuts at: 0 690 948
Size: 690 258
BtgI C'CryG_G
Cuts at: 0 431 948
Size: 431 517
EaeI y'GGCC_r
Cuts at: 0 101 170 334 428 948
Size: 101 69 164 94 520
Fragments arranged by size:
520 164 101 94 69
EarI CTCTTCn'nnn_
Cuts at: 0 223 431 935 948
Size: 223 208 504 13
Fragments arranged by size:
504 223 208 13
EcoICRI GAG'CTC
Cuts at: 0 216 948
Size: 216 732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 319 478 706 906 948
Size: 56 263 159 228 200 42
Fragments arranged by size:
263 228 200 159 56 42
EcoO109I rG'GnC_Cy
Cuts at: 0 258 653 672 741 948
Size: 258 395 19 69 207
Fragments arranged by size:
395 258 207 69 19
HaeII r_GCGC'y
Cuts at: 0 32 948
Size: 32 916
HincII GTy'rAC
Cuts at: 0 546 948
Size: 546 402
HpaI GTT'AAC
Cuts at: 0 546 948
Size: 546 402
KasI G'GCGC_C
Cuts at: 0 28 948
Size: 28 920
KpnI G_GTAC'C
Cuts at: 0 59 120 948
Size: 59 61 828
Fragments arranged by size:
828 61 59
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 948
Size: 503 445
MscI TGG'CCA
Cuts at: 0 103 172 948
Size: 103 69 776
Fragments arranged by size:
776 103 69
MslI CAynn'nnrTG
Cuts at: 0 616 948
Size: 616 332
NarI GG'CG_CC
Cuts at: 0 29 948
Size: 29 919
NcoI C'CATG_G
Cuts at: 0 431 948
Size: 431 517
PasI CC'CwG_GG
Cuts at: 0 656 948
Size: 656 292
PfoI T'CCnGG_A
Cuts at: 0 683 948
Size: 683 265
PmlI CAC'GTG
Cuts at: 0 422 948
Size: 422 526
PpuMI rG'GwC_Cy
Cuts at: 0 672 741 948
Size: 672 69 207
Fragments arranged by size:
672 207 69
PstI C_TGCA'G
Cuts at: 0 543 948
Size: 543 405
SacI G_AGCT'C
Cuts at: 0 218 948
Size: 218 730
SapI GCTCTTCn'nnn_
Cuts at: 0 223 431 948
Size: 223 208 517
Fragments arranged by size:
517 223 208
ScaI AGT'ACT
Cuts at: 0 595 948
Size: 595 353
SfcI C'TryA_G
Cuts at: 0 539 627 731 948
Size: 539 88 104 217
Fragments arranged by size:
539 217 104 88
SfoI GGC'GCC
Cuts at: 0 30 948
Size: 30 918
StyI C'CwwG_G
Cuts at: 0 431 948
Size: 431 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 578 948
Size: 578 370
TatI w'GTAC_w
Cuts at: 0 593 948
Size: 593 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 911 948
Size: 879 32 37
Fragments arranged by size:
879 37 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 438 612 948
Size: 48 390 174 336
Fragments arranged by size:
390 336 174 48
ZraI GAC'GTC
Cuts at: 0 111 948
Size: 111 837
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstEII BstXI
BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
HincII HpaI KasI KpnI MmeI MscI MslI NarI
NcoI PasI PfoI PmlI PpuMI PstI SacI SapI
ScaI SfcI SfoI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII MfeI MluI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI