Restriction map for
pFN21AE2378
(Linear) (Six-base) MAPSORT of: KIEE2378.seq Check: 5712 from: 1 to: 1464
KIEE2378.orf
With 182 enzymes: SgfI *
September 20, 2011 13:23 ..
AflIII A'CryG_T
Cuts at: 0 251 1464
Size: 251 1213
AlwNI CAG_nnn'CTG
Cuts at: 0 358 1270 1464
Size: 358 912 194
Fragments arranged by size:
912 358 194
ApoI r'AATT_y
Cuts at: 0 223 1107 1214 1258 1464
Size: 223 884 107 44 206
Fragments arranged by size:
884 223 206 107 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 481 514 1464
Size: 481 33 950
Fragments arranged by size:
950 481 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1181 1464
Size: 1181 283
BclI T'GATC_A
Cuts at: 0 1308 1464
Size: 1308 156
BlpI GC'TnA_GC
Cuts at: 0 1453 1464
Size: 1453 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 823 1464
Size: 823 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 431 1052 1464
Size: 380 51 621 412
Fragments arranged by size:
621 412 380 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1464
Size: 654 810
BsaBI GATnn'nnATC
Cuts at: 0 533 1464
Size: 533 931
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 914 1464
Size: 914 550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1464
Size: 809 655
BspHI T'CATG_A
Cuts at: 0 1170 1464
Size: 1170 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 604 1464
Size: 604 860
BstYI r'GATC_y
Cuts at: 0 1200 1464
Size: 1200 264
BtsI GCAGTG_nn'
Cuts at: 0 179 1464
Size: 179 1285
DraIII CAC_nnn'GTG
Cuts at: 0 1135 1464
Size: 1135 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 431 1052 1464
Size: 380 51 621 412
Fragments arranged by size:
621 412 380 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 623 1464
Size: 623 841
EcoO109I rG'GnC_Cy
Cuts at: 0 167 1464
Size: 167 1297
EcoRI G'AATT_C
Cuts at: 0 1258 1464
Size: 1258 206
FspI TGC'GCA
Cuts at: 0 1114 1464
Size: 1114 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 238 270 691 723 784 816
Size: 186 32 20 32 421 32 61 32
Cuts at: 816 1464
Size: 648
Fragments arranged by size:
648 421 186 61 32 32 32 32
20
HindIII A'AGCT_T
Cuts at: 0 557 1464
Size: 557 907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 916 1464
Size: 270 646 548
Fragments arranged by size:
646 548 270
MslI CAynn'nnrTG
Cuts at: 0 209 539 542 602 1464
Size: 209 330 3 60 862
Fragments arranged by size:
862 330 209 60 3
NdeI CA'TA_TG
Cuts at: 0 711 942 1464
Size: 711 231 522
Fragments arranged by size:
711 522 231
NspI r_CATG'y
Cuts at: 0 255 1119 1464
Size: 255 864 345
Fragments arranged by size:
864 345 255
PciI A'CATG_T
Cuts at: 0 251 1464
Size: 251 1213
PstI C_TGCA'G
Cuts at: 0 954 1464
Size: 954 510
SexAI A'CCwGG_T
Cuts at: 0 1131 1464
Size: 1131 333
SfcI C'TryA_G
Cuts at: 0 950 1464
Size: 950 514
SmlI C'TyrA_G
Cuts at: 0 633 1464
Size: 633 831
StyI C'CwwG_G
Cuts at: 0 1400 1464
Size: 1400 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1121 1153 1233 1265 1464
Size: 1121 32 80 32 199
Fragments arranged by size:
1121 199 80 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 80 1464
Size: 80 1384
XmnI GAAnn'nnTTC
Cuts at: 0 614 1464
Size: 614 850
Enzymes that do cut:
AflIII AlwNI ApoI BaeI BbsI BclI BlpI BmrI
BpmI BpuEI BsaBI BseRI BsgI BspHI BstXI BstYI
BtsI DraIII Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I
HindIII MmeI MslI NdeI NspI PciI PstI SexAI
SfcI SmlI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI EcoRV
FalI FseI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI XbaI XcmI XhoI XmaI ZraI