(Linear) (Six-base) MAPSORT of: KIEE2376.seq Check: 3474 from: 1 to: 1551 KIEE2376.orf With 182 enzymes: SgfI * September 20, 2011 13:23 .. AccI GT'mk_AC Cuts at: 0 695 1551 Size: 695 856 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1041 1379 1551 Size: 1041 338 172 Fragments arranged by size: 1041 338 172 AflIII A'CryG_T Cuts at: 0 855 1551 Size: 855 696 AvaI C'yCGr_G Cuts at: 0 72 106 259 1551 Size: 72 34 153 1292 Fragments arranged by size: 1292 153 72 34 BanI G'GyrC_C Cuts at: 0 427 1551 Size: 427 1124 BanII G_rGCy'C Cuts at: 0 41 55 104 259 424 482 1551 Size: 41 14 49 155 165 58 1069 Fragments arranged by size: 1069 165 155 58 49 41 14 BbeI G_GCGC'C Cuts at: 0 431 1551 Size: 431 1120 BbsI GAAGACnn'nnnn_ Cuts at: 0 296 1367 1551 Size: 296 1071 184 Fragments arranged by size: 1071 296 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1214 1248 1551 Size: 1214 34 303 Fragments arranged by size: 1214 303 34 BglI GCCn_nnn'nGGC Cuts at: 0 274 377 506 611 1551 Size: 274 103 129 105 940 Fragments arranged by size: 940 274 129 105 103 BglII A'GATC_T Cuts at: 0 828 1551 Size: 828 723 BlpI GC'TnA_GC Cuts at: 0 98 1551 Size: 98 1453 BmrI ACTGGGnnnn_n' Cuts at: 0 581 1551 Size: 581 970 BmtI G_CTAG'C Cuts at: 0 1036 1551 Size: 1036 515 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 57 429 1551 Size: 57 372 1122 Fragments arranged by size: 1122 372 57 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 493 528 710 1551 Size: 52 441 35 182 841 Fragments arranged by size: 841 441 182 52 35 Bpu10I CC'TnA_GC Cuts at: 0 49 646 1551 Size: 49 597 905 Fragments arranged by size: 905 597 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 400 1551 Size: 400 1151 BsaHI Gr'CG_yC Cuts at: 0 428 1551 Size: 428 1123 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 613 643 1551 Size: 613 30 908 Fragments arranged by size: 908 613 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 124 127 130 139 142 145 154 Size: 49 75 3 3 9 3 3 9 Cuts at: 154 230 310 313 325 328 364 367 490 Size: 76 80 3 12 3 36 3 123 Cuts at: 490 887 1062 1551 Size: 397 175 489 Fragments arranged by size: 489 397 175 123 80 76 75 49 36 12 9 9 3 3 3 3 3 3 3 BsiHKAI G_wGCw'C Cuts at: 0 41 1551 Size: 41 1510 BsmBI CGTCTCn'nnnn_ Cuts at: 0 232 1551 Size: 232 1319 Bsp1286I G_dGCh'C Cuts at: 0 41 55 104 259 424 482 1551 Size: 41 14 49 155 165 58 1069 Fragments arranged by size: 1069 165 155 58 49 41 14 BsrBI CCG'CTC Cuts at: 0 413 1551 Size: 413 1138 BsrDI GCAATG_nn' Cuts at: 0 737 1011 1551 Size: 737 274 540 Fragments arranged by size: 737 540 274 BsrFI r'CCGG_y Cuts at: 0 605 1551 Size: 605 946 BstYI r'GATC_y Cuts at: 0 828 1551 Size: 828 723 BtgI C'CryG_G Cuts at: 0 490 1174 1551 Size: 490 684 377 Fragments arranged by size: 684 490 377 DraIII CAC_nnn'GTG Cuts at: 0 967 1551 Size: 967 584 EaeI y'GGCC_r Cuts at: 0 2 23 1177 1551 Size: 2 21 1154 374 Fragments arranged by size: 1154 374 21 2 EarI CTCTTCn'nnn_ Cuts at: 0 116 131 1489 1498 1551 Size: 116 15 1358 9 53 Fragments arranged by size: 1358 116 53 15 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 160 215 227 596 1551 Size: 160 55 12 369 955 Fragments arranged by size: 955 369 160 55 12 EcoICRI GAG'CTC Cuts at: 0 39 1551 Size: 39 1512 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 493 528 710 1041 1379 1551 Size: 52 441 35 182 331 338 172 Fragments arranged by size: 441 338 331 182 172 52 35 EcoO109I rG'GnC_Cy Cuts at: 0 468 1551 Size: 468 1083 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 773 1551 Size: 773 778 HaeII r_GCGC'y Cuts at: 0 169 269 365 431 1551 Size: 169 100 96 66 1120 Fragments arranged by size: 1120 169 100 96 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 261 613 645 1206 1238 1551 Size: 229 32 352 32 561 32 313 Fragments arranged by size: 561 352 313 229 32 32 32 KasI G'GCGC_C Cuts at: 0 427 1551 Size: 427 1124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 227 611 1551 Size: 227 384 940 Fragments arranged by size: 940 384 227 MslI CAynn'nnrTG Cuts at: 0 19 860 1551 Size: 19 841 691 Fragments arranged by size: 841 691 19 MspA1I CmG'CkG Cuts at: 0 188 338 386 446 492 548 1551 Size: 188 150 48 60 46 56 1003 Fragments arranged by size: 1003 188 150 60 56 48 46 NaeI GCC'GGC Cuts at: 0 607 1551 Size: 607 944 NarI GG'CG_CC Cuts at: 0 428 1551 Size: 428 1123 NdeI CA'TA_TG Cuts at: 0 953 1231 1551 Size: 953 278 320 Fragments arranged by size: 953 320 278 NgoMIV G'CCGG_C Cuts at: 0 605 1551 Size: 605 946 NheI G'CTAG_C Cuts at: 0 1032 1551 Size: 1032 519 NspI r_CATG'y Cuts at: 0 859 1551 Size: 859 692 PciI A'CATG_T Cuts at: 0 855 1551 Size: 855 696 SacI G_AGCT'C Cuts at: 0 41 1551 Size: 41 1510 SacII CC_GC'GG Cuts at: 0 493 1551 Size: 493 1058 ScaI AGT'ACT Cuts at: 0 681 1296 1551 Size: 681 615 255 Fragments arranged by size: 681 615 255 SfoI GGC'GCC Cuts at: 0 429 1551 Size: 429 1122 SmaI CCC'GGG Cuts at: 0 74 1551 Size: 74 1477 SspI AAT'ATT Cuts at: 0 774 1551 Size: 774 777 StyI C'CwwG_G Cuts at: 0 482 1551 Size: 482 1069 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1371 1551 Size: 1371 180 TatI w'GTAC_w Cuts at: 0 679 1294 1551 Size: 679 615 257 Fragments arranged by size: 679 615 257 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 832 864 1551 Size: 832 32 687 Fragments arranged by size: 832 687 32 Tth111I GACn'n_nGTC Cuts at: 0 243 1551 Size: 243 1308 XmaI C'CCGG_G Cuts at: 0 72 1551 Size: 72 1479 XmnI GAAnn'nnTTC Cuts at: 0 1364 1551 Size: 1364 187 Enzymes that do cut: AccI AcuI AflIII AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI BmrI BmtI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstYI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI PciI SacI SacII ScaI SfoI SmaI SspI StyI TaqII TatI TstI Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI Bme1580I BmgBI BpuEI BsaAI BsaBI BsaWI BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI ZraI