(Linear) (Six-base) MAPSORT of: KIEE2366.seq  Check: 2125  from: 1  to: 1398



KIEE2366.orf

 With 182 enzymes: SgfI * 

                        August 4, 2011 11:52  ..

AccI GT'mk_AC

Cuts at:      0      19    1398 
   Size:         19    1379
 
AleI CACnn'nnGTG

Cuts at:      0     673    1398 
   Size:        673     725
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66    1398 
   Size:         66    1332
 
ApoI r'AATT_y

Cuts at:      0     589    1398 
   Size:        589     809
 
BlpI GC'TnA_GC

Cuts at:      0      67    1398 
   Size:         67    1331
 
Bme1580I G_kGCm'C

Cuts at:      0     704    1398 
   Size:        704     694
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1103    1398 
   Size:       1103     295
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     497    1398 
   Size:        497     901
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1398 
   Size:        122    1276
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247    1398 
   Size:        247    1151
 
BsaAI yAC'GTr

Cuts at:      0     414    1398 
   Size:        414     984
 
BsaBI GATnn'nnATC

Cuts at:      0     608     731     839    1398 
   Size:        608     123     108     559

  Fragments arranged by size: 

                 608     559     123     108
 
BsaWI w'CCGG_w

Cuts at:      0     395    1398 
   Size:        395    1003
 
BseYI C'CCAG_C

Cuts at:      0    1382    1398 
   Size:       1382      16
 
BsmI GAATG_Cn'

Cuts at:      0     819    1343    1398 
   Size:        819     524      55

  Fragments arranged by size: 

                 819     524      55
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      98    1398 
   Size:         98    1300
 
Bsp1286I G_dGCh'C

Cuts at:      0     704    1398 
   Size:        704     694
 
BsrDI GCAATG_nn'

Cuts at:      0    1003    1398 
   Size:       1003     395
 
BsrFI r'CCGG_y

Cuts at:      0      27    1398 
   Size:         27    1371
 
BsrGI T'GTAC_A

Cuts at:      0    1276    1398 
   Size:       1276     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1200    1398 
   Size:       1200     198
 
BstYI r'GATC_y

Cuts at:      0     324     499     603    1008    1257    1398 
   Size:        324     175     104     405     249     141

  Fragments arranged by size: 

                 405     324     249     175     141     104
 
BtgI C'CryG_G

Cuts at:      0     564    1398 
   Size:        564     834
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     951    1398 
   Size:        951     447
 
BtsI GCAGTG_nn'

Cuts at:      0      78     547    1398 
   Size:         78     469     851

  Fragments arranged by size: 

                 851     469      78
 
EaeI y'GGCC_r

Cuts at:      0      50     561     969    1398 
   Size:         50     511     408     429

  Fragments arranged by size: 

                 511     429     408      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1398 
   Size:        122    1276
 
HaeII r_GCGC'y

Cuts at:      0    1383    1398 
   Size:       1383      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542     654     686    1000    1032    1398 
   Size:        510      32     112      32     314      32     366

  Fragments arranged by size: 

                 510     366     314     112      32      32      32
 
HincII GTy'rAC

Cuts at:      0      20    1398 
   Size:         20    1378
 
MscI TGG'CCA

Cuts at:      0     563    1398 
   Size:        563     835
 
MslI CAynn'nnrTG

Cuts at:      0     569     673     707    1298    1398 
   Size:        569     104      34     591     100

  Fragments arranged by size: 

                 591     569     104     100      34
 
NaeI GCC'GGC

Cuts at:      0      29    1398 
   Size:         29    1369
 
NcoI C'CATG_G

Cuts at:      0     564    1398 
   Size:        564     834
 
NgoMIV G'CCGG_C

Cuts at:      0      27    1398 
   Size:         27    1371
 
NspI r_CATG'y

Cuts at:      0      45    1398 
   Size:         45    1353
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     289    1376    1398 
   Size:        289    1087      22

  Fragments arranged by size: 

                1087     289      22
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     698     730    1398 
   Size:        698      32     668

  Fragments arranged by size: 

                 698     668      32
 
PsiI TTA'TAA

Cuts at:      0     839    1398 
   Size:        839     559
 
SalI G'TCGA_C

Cuts at:      0      18    1398 
   Size:         18    1380
 
SfcI C'TryA_G

Cuts at:      0     344     491    1398 
   Size:        344     147     907

  Fragments arranged by size: 

                 907     344     147
 
SgrAI Cr'CCGG_yG

Cuts at:      0      27    1398 
   Size:         27    1371
 
SmlI C'TyrA_G

Cuts at:      0     262    1398 
   Size:        262    1136
 
SspI AAT'ATT

Cuts at:      0     238     683    1398 
   Size:        238     445     715

  Fragments arranged by size: 

                 715     445     238
 
StyI C'CwwG_G

Cuts at:      0     564    1398 
   Size:        564     834
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     730    1398 
   Size:        730     668
 
TatI w'GTAC_w

Cuts at:      0    1276    1398 
   Size:       1276     122
 
Tth111I GACn'n_nGTC

Cuts at:      0     121    1398 
   Size:        121    1277
 
XbaI T'CTAG_A

Cuts at:      0     502    1398 
   Size:        502     896
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     286    1398 
   Size:        286    1112
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApoI     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BseYI     BsmI    BsmBI
 Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI    BstYI     BtgI    BtgZI
     BtsI     EaeI  Eco57MI    HaeII    Hin4I   HincII     MscI     MslI
     NaeI     NcoI   NgoMIV     NspI    PflMI     PpiI     PsiI     SalI
     SfcI    SgrAI     SmlI     SspI     StyI    TaqII     TatI  Tth111I
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmtI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrBI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI     XhoI     XmaI
     XmnI     ZraI