(Linear) (Six-base) MAPSORT of: KIEE2362.seq  Check: 8282  from: 1  to: 825



KIEE2362.orf

 With 182 enzymes: SgfI * 

                        July 21, 2011 14:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     707     825 
   Size:        707     118
 
AlwNI CAG_nnn'CTG

Cuts at:      0     603     759     825 
   Size:        603     156      66

  Fragments arranged by size: 

                 603     156      66
 
ApoI r'AATT_y

Cuts at:      0      44     825 
   Size:         44     781
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     194     825 
   Size:        194     631
 
BglII A'GATC_T

Cuts at:      0     648     825 
   Size:        648     177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     670     825 
   Size:        670     155
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     643     825 
   Size:        643     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     825 
   Size:        322     503
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     233     825 
   Size:        233     592
 
BstYI r'GATC_y

Cuts at:      0     648     825 
   Size:        648     177
 
BtsI GCAGTG_nn'

Cuts at:      0     760     825 
   Size:        760      65
 
DraI TTT'AAA

Cuts at:      0     404     825 
   Size:        404     421
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     825 
   Size:        290     535
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     707     825 
   Size:        707     118
 
EcoRI G'AATT_C

Cuts at:      0      44     825 
   Size:         44     781
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      37     825 
   Size:         37     788
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     753     785     825 
   Size:        753      32      40

  Fragments arranged by size: 

                 753      40      32
 
MfeI C'AATT_G

Cuts at:      0     245     359     825 
   Size:        245     114     466

  Fragments arranged by size: 

                 466     245     114
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     719     825 
   Size:         73     646     106

  Fragments arranged by size: 

                 646     106      73
 
MslI CAynn'nnrTG

Cuts at:      0     231     825 
   Size:        231     594
 
PstI C_TGCA'G

Cuts at:      0     531     624     755     825 
   Size:        531      93     131      70

  Fragments arranged by size: 

                 531     131      93      70
 
SapI GCTCTTCn'nnn_

Cuts at:      0     290     825 
   Size:        290     535
 
SfcI C'TryA_G

Cuts at:      0     527     620     751     825 
   Size:        527      93     131      74

  Fragments arranged by size: 

                 527     131      93      74
 
StyI C'CwwG_G

Cuts at:      0     289     825 
   Size:        289     536
 
TatI w'GTAC_w

Cuts at:      0     303     825 
   Size:        303     522
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BbsI    BglII     BplI    BseRI     BsgI
    BstXI    BstYI     BtsI     DraI     EarI  Eco57MI    EcoRI     FalI
    Hin4I     MfeI     MmeI     MslI     PstI     SapI     SfcI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI