Restriction map for
pFN21AE2361
(Linear) (Six-base) MAPSORT of: KIEE2361.seq Check: 8384 from: 1 to: 792
KIEE2361.orf
With 182 enzymes: SgfI *
July 21, 2011 14:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 559 617 792
Size: 469 90 58 175
Fragments arranged by size:
469 175 90 58
AfeI AGC'GCT
Cuts at: 0 221 792
Size: 221 571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 155 766 792
Size: 155 611 26
Fragments arranged by size:
611 155 26
AlwNI CAG_nnn'CTG
Cuts at: 0 621 780 792
Size: 621 159 12
Fragments arranged by size:
621 159 12
ApaI G_GGCC'C
Cuts at: 0 589 792
Size: 589 203
BanI G'GyrC_C
Cuts at: 0 31 121 742 792
Size: 31 90 621 50
Fragments arranged by size:
621 90 50 31
BanII G_rGCy'C
Cuts at: 0 589 792
Size: 589 203
BglI GCCn_nnn'nGGC
Cuts at: 0 93 102 287 556 792
Size: 93 9 185 269 236
Fragments arranged by size:
269 236 185 93 9
BlpI GC'TnA_GC
Cuts at: 0 11 792
Size: 11 781
Bme1580I G_kGCm'C
Cuts at: 0 72 589 745 792
Size: 72 517 156 47
Fragments arranged by size:
517 156 72 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 792
Size: 37 755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 792
Size: 200 592
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 792
Size: 74 718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 792
Size: 381 411
BsiHKAI G_wGCw'C
Cuts at: 0 716 792
Size: 716 76
Bsp1286I G_dGCh'C
Cuts at: 0 72 589 716 745 792
Size: 72 517 127 29 47
Fragments arranged by size:
517 127 72 47 29
BspHI T'CATG_A
Cuts at: 0 387 792
Size: 387 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 423 792
Size: 423 369
BsrBI CCG'CTC
Cuts at: 0 29 187 792
Size: 29 158 605
Fragments arranged by size:
605 158 29
BsrDI GCAATG_nn'
Cuts at: 0 605 792
Size: 605 187
BstEII G'GTnAC_C
Cuts at: 0 501 577 792
Size: 501 76 215
Fragments arranged by size:
501 215 76
BstYI r'GATC_y
Cuts at: 0 617 792
Size: 617 175
Bsu36I CC'TnA_GG
Cuts at: 0 698 792
Size: 698 94
BtsI GCAGTG_nn'
Cuts at: 0 479 792
Size: 479 313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 205 240 792
Size: 205 35 552
Fragments arranged by size:
552 205 35
EaeI y'GGCC_r
Cuts at: 0 360 557 642 792
Size: 360 197 85 150
Fragments arranged by size:
360 197 150 85
EarI CTCTTCn'nnn_
Cuts at: 0 593 792
Size: 593 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 469 559 617 792
Size: 37 432 90 58 175
Fragments arranged by size:
432 175 90 58 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 792
Size: 116 676
EcoO109I rG'GnC_Cy
Cuts at: 0 444 586 792
Size: 444 142 206
Fragments arranged by size:
444 206 142
HaeII r_GCGC'y
Cuts at: 0 11 223 787 792
Size: 11 212 564 5
Fragments arranged by size:
564 212 11 5
MscI TGG'CCA
Cuts at: 0 559 792
Size: 559 233
MspA1I CmG'CkG
Cuts at: 0 15 792
Size: 15 777
PspOMI G'GGCC_C
Cuts at: 0 585 792
Size: 585 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 562 594 792
Size: 562 32 198
Fragments arranged by size:
562 198 32
PstI C_TGCA'G
Cuts at: 0 144 792
Size: 144 648
PvuII CAG'CTG
Cuts at: 0 15 792
Size: 15 777
SfcI C'TryA_G
Cuts at: 0 140 270 303 792
Size: 140 130 33 489
Fragments arranged by size:
489 140 130 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 102 556 792
Size: 102 454 236
Fragments arranged by size:
454 236 102
SmlI C'TyrA_G
Cuts at: 0 215 792
Size: 215 577
StyI C'CwwG_G
Cuts at: 0 494 792
Size: 494 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 47 792
Size: 47 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 495 527 792
Size: 495 32 265
Fragments arranged by size:
495 265 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 792
Size: 618 174
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI BanI BanII BglI
BlpI Bme1580I BpmI BpuEI BsaI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BstEII BstYI Bsu36I BtsI
CspCI EaeI EarI Eco57MI EcoNI EcoO109I HaeII MscI
MspA1I PspOMI PsrI PstI PvuII SfcI SfiI SmlI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI