Restriction map for
pFN21AE2358
(Linear) (Six-base) MAPSORT of: KIEE2358.seq Check: 4449 from: 1 to: 1263
KIEE2358.orf
With 182 enzymes: SgfI *
July 7, 2011 16:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1263
Size: 754 509
AfeI AGC'GCT
Cuts at: 0 379 1263
Size: 379 884
AflIII A'CryG_T
Cuts at: 0 485 1263
Size: 485 778
AgeI A'CCGG_T
Cuts at: 0 51 1263
Size: 51 1212
AleI CACnn'nnGTG
Cuts at: 0 1229 1263
Size: 1229 34
AlwNI CAG_nnn'CTG
Cuts at: 0 460 1263
Size: 460 803
ApaI G_GGCC'C
Cuts at: 0 467 1263
Size: 467 796
ApoI r'AATT_y
Cuts at: 0 689 1263
Size: 689 574
AvaI C'yCGr_G
Cuts at: 0 256 346 394 1263
Size: 256 90 48 869
Fragments arranged by size:
869 256 90 48
BanII G_rGCy'C
Cuts at: 0 26 230 257 467 710 1163 1170 1263
Size: 26 204 27 210 243 453 7 93
Fragments arranged by size:
453 243 210 204 93 27 26 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 1263
Size: 587 676
BglI GCCn_nnn'nGGC
Cuts at: 0 234 1263
Size: 234 1029
Bme1580I G_kGCm'C
Cuts at: 0 467 1079 1263
Size: 467 612 184
Fragments arranged by size:
612 467 184
BmgBI CAC'GTC
Cuts at: 0 291 1263
Size: 291 972
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 457 1263
Size: 457 806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 205 337 518 532 634 1189 1263
Size: 40 165 132 181 14 102 555 74
Fragments arranged by size:
555 181 165 132 102 74 40 14
Bpu10I CC'TnA_GC
Cuts at: 0 1181 1263
Size: 1181 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1263
Size: 244 1019
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 1263
Size: 802 461
BsaHI Gr'CG_yC
Cuts at: 0 827 1263
Size: 827 436
BsaWI w'CCGG_w
Cuts at: 0 51 1263
Size: 51 1212
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1053 1263
Size: 1053 210
BseYI C'CCAG_C
Cuts at: 0 41 201 535 1263
Size: 41 160 334 728
Fragments arranged by size:
728 334 160 41
BsiHKAI G_wGCw'C
Cuts at: 0 710 949 1263
Size: 710 239 314
Fragments arranged by size:
710 314 239
BsmBI CGTCTCn'nnnn_
Cuts at: 0 595 1263
Size: 595 668
Bsp1286I G_dGCh'C
Cuts at: 0 26 230 257 467 710 949 1079 1163
Size: 26 204 27 210 243 239 130 84
Cuts at: 1163 1170 1263
Size: 7 93
Fragments arranged by size:
243 239 210 204 130 93 84 27
26 7
BsrFI r'CCGG_y
Cuts at: 0 51 90 411 1263
Size: 51 39 321 852
Fragments arranged by size:
852 321 51 39
BssHII G'CGCG_C
Cuts at: 0 102 128 148 189 191 417 1263
Size: 102 26 20 41 2 226 846
Fragments arranged by size:
846 226 102 41 26 20 2
BssSI C'ACGA_G
Cuts at: 0 373 1237 1263
Size: 373 864 26
Fragments arranged by size:
864 373 26
BstEII G'GTnAC_C
Cuts at: 0 849 1263
Size: 849 414
BstXI CCAn_nnnn'nTGG
Cuts at: 0 597 1263
Size: 597 666
Bsu36I CC'TnA_GG
Cuts at: 0 656 1263
Size: 656 607
BtgI C'CryG_G
Cuts at: 0 26 230 308 596 719 1032 1175 1263
Size: 26 204 78 288 123 313 143 88
Fragments arranged by size:
313 288 204 143 123 88 78 26
EaeI y'GGCC_r
Cuts at: 0 305 330 773 1233 1263
Size: 305 25 443 460 30
Fragments arranged by size:
460 443 305 30 25
EarI CTCTTCn'nnn_
Cuts at: 0 448 910 982 1263
Size: 448 462 72 281
Fragments arranged by size:
462 448 281 72
EcoICRI GAG'CTC
Cuts at: 0 708 1263
Size: 708 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 205 337 518 532 634 754 1189
Size: 40 165 132 181 14 102 120 435
Cuts at: 1189 1263
Size: 74
Fragments arranged by size:
435 181 165 132 120 102 74 40
14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 899 1263
Size: 899 364
EcoO109I rG'GnC_Cy
Cuts at: 0 464 904 1263
Size: 464 440 359
Fragments arranged by size:
464 440 359
HaeII r_GCGC'y
Cuts at: 0 381 1263
Size: 381 882
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 1263
Size: 594 32 637
Fragments arranged by size:
637 594 32
HincII GTy'rAC
Cuts at: 0 831 1263
Size: 831 432
MscI TGG'CCA
Cuts at: 0 332 775 1235 1263
Size: 332 443 460 28
Fragments arranged by size:
460 443 332 28
MslI CAynn'nnrTG
Cuts at: 0 847 928 1229 1263
Size: 847 81 301 34
Fragments arranged by size:
847 301 81 34
MspA1I CmG'CkG
Cuts at: 0 28 35 1263
Size: 28 7 1228
Fragments arranged by size:
1228 28 7
NcoI C'CATG_G
Cuts at: 0 230 596 719 1032 1175 1263
Size: 230 366 123 313 143 88
Fragments arranged by size:
366 313 230 143 123 88
NspI r_CATG'y
Cuts at: 0 489 1126 1263
Size: 489 637 137
Fragments arranged by size:
637 489 137
PasI CC'CwG_GG
Cuts at: 0 1163 1263
Size: 1163 100
PciI A'CATG_T
Cuts at: 0 485 1263
Size: 485 778
PflMI CCAn_nnn'nTGG
Cuts at: 0 460 676 1263
Size: 460 216 587
Fragments arranged by size:
587 460 216
PfoI T'CCnGG_A
Cuts at: 0 17 669 1263
Size: 17 652 594
Fragments arranged by size:
652 594 17
PpuMI rG'GwC_Cy
Cuts at: 0 904 1263
Size: 904 359
PspOMI G'GGCC_C
Cuts at: 0 463 1263
Size: 463 800
SacI G_AGCT'C
Cuts at: 0 710 1263
Size: 710 553
SacII CC_GC'GG
Cuts at: 0 29 1263
Size: 29 1234
SapI GCTCTTCn'nnn_
Cuts at: 0 448 910 1263
Size: 448 462 353
Fragments arranged by size:
462 448 353
ScaI AGT'ACT
Cuts at: 0 619 1263
Size: 619 644
SmaI CCC'GGG
Cuts at: 0 348 396 1263
Size: 348 48 867
Fragments arranged by size:
867 348 48
SmlI C'TyrA_G
Cuts at: 0 223 1263
Size: 223 1040
SphI G_CATG'C
Cuts at: 0 1126 1263
Size: 1126 137
StuI AGG'CCT
Cuts at: 0 1012 1263
Size: 1012 251
StyI C'CwwG_G
Cuts at: 0 168 230 248 266 302 596 719 1032
Size: 168 62 18 18 36 294 123 313
Cuts at: 1032 1127 1175 1263
Size: 95 48 88
Fragments arranged by size:
313 294 168 123 95 88 62 48
36 18 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1219 1263
Size: 1219 44
TatI w'GTAC_w
Cuts at: 0 617 1263
Size: 617 646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1182 1263
Size: 1182 81
XmaI C'CCGG_G
Cuts at: 0 346 394 1263
Size: 346 48 869
Fragments arranged by size:
869 346 48
XmnI GAAnn'nnTTC
Cuts at: 0 919 1263
Size: 919 344
Enzymes that do cut:
AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApoI
AvaI BanII BbsI BglI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BsrFI BssHII BssSI BstEII BstXI Bsu36I
BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII MscI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpuMI PspOMI SacI SacII SapI
ScaI SmaI SmlI SphI StuI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XhoI
ZraI