Restriction map for
pFN21AE2355
(Linear) (Six-base) MAPSORT of: KIEE2355.seq Check: 8724 from: 1 to: 2886
KIEE2355.orf
With 182 enzymes: SgfI *
June 21, 2011 13:28 ..
AatII G_ACGT'C
Cuts at: 0 1193 2886
Size: 1193 1693
Acc65I G'GTAC_C
Cuts at: 0 526 2886
Size: 526 2360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 2578 2809 2886
Size: 609 1969 231 77
Fragments arranged by size:
1969 609 231 77
AleI CACnn'nnGTG
Cuts at: 0 13 2886
Size: 13 2873
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 2886
Size: 1286 32 1568
Fragments arranged by size:
1568 1286 32
AlwNI CAG_nnn'CTG
Cuts at: 0 619 2446 2886
Size: 619 1827 440
Fragments arranged by size:
1827 619 440
ApaI G_GGCC'C
Cuts at: 0 270 915 1081 1358 1525 2223 2886
Size: 270 645 166 277 167 698 663
Fragments arranged by size:
698 663 645 277 270 167 166
ApaLI G'TGCA_C
Cuts at: 0 768 2886
Size: 768 2118
ApoI r'AATT_y
Cuts at: 0 85 2886
Size: 85 2801
AvaI C'yCGr_G
Cuts at: 0 982 1030 1240 1378 1402 1426 1464 1858
Size: 982 48 210 138 24 24 38 394
Cuts at: 1858 1917 2022 2188 2257 2349 2590 2886
Size: 59 105 166 69 92 241 296
Fragments arranged by size:
982 394 296 241 210 166 138 105
92 69 59 48 38 24 24
BanI G'GyrC_C
Cuts at: 0 526 609 855 888 1122 1269 1368 1455
Size: 526 83 246 33 234 147 99 87
Cuts at: 1455 1476 1638 1651 1665 1693 2092 2212 2586
Size: 21 162 13 14 28 399 120 374
Cuts at: 2586 2886
Size: 300
Fragments arranged by size:
526 399 374 300 246 234 162 147
120 99 87 83 33 28 21 14
13
BanII G_rGCy'C
Cuts at: 0 270 463 490 915 1022 1031 1061 1081
Size: 270 193 27 425 107 9 30 20
Cuts at: 1081 1201 1358 1379 1403 1427 1448 1471 1525
Size: 120 157 21 24 24 21 23 54
Cuts at: 1525 1574 1865 1960 1997 2223 2378 2886
Size: 49 291 95 37 226 155 508
Fragments arranged by size:
508 425 291 270 226 193 157 155
120 107 95 54 49 37 30 27
24 24 23 21 21 20 9
BbeI G_GCGC'C
Cuts at: 0 859 892 1126 1273 1372 1459 1480 1642
Size: 859 33 234 147 99 87 21 162
Cuts at: 1642 1655 1669 2096 2886
Size: 13 14 427 790
Fragments arranged by size:
859 790 427 234 162 147 99 87
33 21 14 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1193 2540 2886
Size: 1193 1347 346
Fragments arranged by size:
1347 1193 346
BglI GCCn_nnn'nGGC
Cuts at: 0 1362 1660 1939 2886
Size: 1362 298 279 947
Fragments arranged by size:
1362 947 298 279
BlpI GC'TnA_GC
Cuts at: 0 671 2266 2886
Size: 671 1595 620
Fragments arranged by size:
1595 671 620
Bme1580I G_kGCm'C
Cuts at: 0 253 270 772 915 1081 1146 1358 1525
Size: 253 17 502 143 166 65 212 167
Cuts at: 1525 2223 2550 2591 2886
Size: 698 327 41 295
Fragments arranged by size:
698 502 327 295 253 212 167 166
143 65 41 17
BmgBI CAC'GTC
Cuts at: 0 516 767 852 2886
Size: 516 251 85 2034
Fragments arranged by size:
2034 516 251 85
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1290 2886
Size: 1011 279 1596
Fragments arranged by size:
1596 1011 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 401 539 667 1831 1839 2468 2886
Size: 256 145 138 128 1164 8 629 418
Fragments arranged by size:
1164 629 418 256 145 138 128 8
Bpu10I CC'TnA_GC
Cuts at: 0 754 872 1373 2886
Size: 754 118 501 1513
Fragments arranged by size:
1513 754 501 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 2837 2886
Size: 981 1856 49
Fragments arranged by size:
1856 981 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 546 1858 2886
Size: 546 1312 1028
Fragments arranged by size:
1312 1028 546
BsaAI yAC'GTr
Cuts at: 0 564 2886
Size: 564 2322
BsaHI Gr'CG_yC
Cuts at: 0 856 889 977 1123 1190 1270 1324 1369
Size: 856 33 88 146 67 80 54 45
Cuts at: 1369 1456 1477 1639 1652 1666 1757 2093 2342
Size: 87 21 162 13 14 91 336 249
Cuts at: 2342 2886
Size: 544
Fragments arranged by size:
856 544 336 249 162 146 91 88
87 80 67 54 45 33 21 14
13
BsaWI w'CCGG_w
Cuts at: 0 301 2057 2138 2675 2886
Size: 301 1756 81 537 211
Fragments arranged by size:
1756 537 301 211 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1048 1078 2164 2194 2886
Size: 1048 30 1086 30 692
Fragments arranged by size:
1086 1048 692 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 404 636 793 796 808 933 982
Size: 253 151 232 157 3 12 125 49
Cuts at: 982 1180 1360 1424 1818 1836 2188 2886
Size: 198 180 64 394 18 352 698
Fragments arranged by size:
698 394 352 253 232 198 180 157
151 125 64 49 18 12 3
BseYI C'CCAG_C
Cuts at: 0 255 832 1865 2001 2224 2886
Size: 255 577 1033 136 223 662
Fragments arranged by size:
1033 662 577 255 223 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 2886
Size: 493 2393
BsiEI CG_ry'CG
Cuts at: 0 709 1511 1788 1943 1973 2886
Size: 709 802 277 155 30 913
Fragments arranged by size:
913 802 709 277 155 30
BsiHKAI G_wGCw'C
Cuts at: 0 463 772 1022 1182 1337 2165 2886
Size: 463 309 250 160 155 828 721
Fragments arranged by size:
828 721 463 309 250 160 155
BsmBI CGTCTCn'nnnn_
Cuts at: 0 585 1387 1764 2886
Size: 585 802 377 1122
Fragments arranged by size:
1122 802 585 377
Bsp1286I G_dGCh'C
Cuts at: 0 253 270 463 490 772 915 1022 1031
Size: 253 17 193 27 282 143 107 9
Cuts at: 1031 1061 1081 1146 1182 1201 1337 1358 1379
Size: 30 20 65 36 19 136 21 21
Cuts at: 1379 1403 1427 1448 1471 1525 1574 1865 1960
Size: 24 24 21 23 54 49 291 95
Cuts at: 1960 1997 2165 2223 2378 2550 2591 2886
Size: 37 168 58 155 172 41 295
Fragments arranged by size:
295 291 282 253 193 172 168 155
143 136 107 95 65 58 54 49
41 37 36 30 27 24 24 23
21 21 21 20 19 17 9
BspEI T'CCGG_A
Cuts at: 0 301 2886
Size: 301 2585
BsrBI CCG'CTC
Cuts at: 0 1414 1674 1808 2265 2358 2886
Size: 1414 260 134 457 93 528
Fragments arranged by size:
1414 528 457 260 134 93
BsrDI GCAATG_nn'
Cuts at: 0 188 2886
Size: 188 2698
BsrFI r'CCGG_y
Cuts at: 0 451 885 1365 1473 1499 1644 2016 2344
Size: 451 434 480 108 26 145 372 328
Cuts at: 2344 2886
Size: 542
Fragments arranged by size:
542 480 451 434 372 328 145 108
26
BssHII G'CGCG_C
Cuts at: 0 735 2886
Size: 735 2151
BssSI C'ACGA_G
Cuts at: 0 1731 1986 2886
Size: 1731 255 900
Fragments arranged by size:
1731 900 255
BstYI r'GATC_y
Cuts at: 0 590 2886
Size: 590 2296
Bsu36I CC'TnA_GG
Cuts at: 0 1220 1910 1961 2886
Size: 1220 690 51 925
Fragments arranged by size:
1220 925 690 51
BtgI C'CryG_G
Cuts at: 0 1151 1451 1505 2886
Size: 1151 300 54 1381
Fragments arranged by size:
1381 1151 300 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 716 2886
Size: 716 2170
BtsI GCAGTG_nn'
Cuts at: 0 1305 2765 2886
Size: 1305 1460 121
Fragments arranged by size:
1460 1305 121
DraIII CAC_nnn'GTG
Cuts at: 0 516 2886
Size: 516 2370
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 2559 2886
Size: 577 1982 327
Fragments arranged by size:
1982 577 327
EaeI y'GGCC_r
Cuts at: 0 956 1007 1148 1508 1785 1931 1940 1970
Size: 956 51 141 360 277 146 9 30
Cuts at: 1970 2886
Size: 916
Fragments arranged by size:
956 916 360 277 146 141 51 30
9
EagI C'GGCC_G
Cuts at: 0 1508 1785 1940 1970 2886
Size: 1508 277 155 30 916
Fragments arranged by size:
1508 916 277 155 30
EarI CTCTTCn'nnn_
Cuts at: 0 115 818 2113 2785 2886
Size: 115 703 1295 672 101
Fragments arranged by size:
1295 703 672 115 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1118 1177 1696 1912 2401 2886
Size: 1118 59 519 216 489 485
Fragments arranged by size:
1118 519 489 485 216 59
EcoICRI GAG'CTC
Cuts at: 0 461 1020 2886
Size: 461 559 1866
Fragments arranged by size:
1866 559 461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 401 539 609 667 1831 1839 2468
Size: 256 145 138 70 58 1164 8 629
Cuts at: 2468 2578 2809 2886
Size: 110 231 77
Fragments arranged by size:
1164 629 256 231 145 138 110 77
70 58 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 416 2886
Size: 55 361 2470
Fragments arranged by size:
2470 361 55
EcoO109I rG'GnC_Cy
Cuts at: 0 267 520 868 911 990 1077 1078 1354
Size: 267 253 348 43 79 87 1 276
Cuts at: 1354 1355 1521 1522 2219 2220 2443 2507 2886
Size: 1 166 1 697 1 223 64 379
Fragments arranged by size:
697 379 348 276 267 253 223 166
87 79 64 43 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2715 2886
Size: 2715 171
FspI TGC'GCA
Cuts at: 0 2404 2886
Size: 2404 482
HaeII r_GCGC'y
Cuts at: 0 831 859 892 1126 1273 1372 1459 1480
Size: 831 28 33 234 147 99 87 21
Cuts at: 1480 1617 1642 1655 1669 2096 2886
Size: 137 25 13 14 427 790
Fragments arranged by size:
831 790 427 234 147 137 99 87
33 28 25 21 14 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 1018 1048 1050 1080 2080 2112
Size: 351 32 635 30 2 30 1000 32
Cuts at: 2112 2806 2838 2886
Size: 694 32 48
Fragments arranged by size:
1000 694 635 351 48 32 32 32
30 30 2
HindIII A'AGCT_T
Cuts at: 0 28 2855 2886
Size: 28 2827 31
Fragments arranged by size:
2827 31 28
KasI G'GCGC_C
Cuts at: 0 855 888 1122 1269 1368 1455 1476 1638
Size: 855 33 234 147 99 87 21 162
Cuts at: 1638 1651 1665 2092 2886
Size: 13 14 427 794
Fragments arranged by size:
855 794 427 234 162 147 99 87
33 21 14 13
KpnI G_GTAC'C
Cuts at: 0 530 2886
Size: 530 2356
MslI CAynn'nnrTG
Cuts at: 0 13 2886
Size: 13 2873
MspA1I CmG'CkG
Cuts at: 0 705 1507 1549 1770 1784 2400 2668 2787
Size: 705 802 42 221 14 616 268 119
Cuts at: 2787 2886
Size: 99
Fragments arranged by size:
802 705 616 268 221 119 99 42
14
NaeI GCC'GGC
Cuts at: 0 453 1367 1475 2018 2346 2886
Size: 453 914 108 543 328 540
Fragments arranged by size:
914 543 540 453 328 108
NarI GG'CG_CC
Cuts at: 0 856 889 1123 1270 1369 1456 1477 1639
Size: 856 33 234 147 99 87 21 162
Cuts at: 1639 1652 1666 2093 2886
Size: 13 14 427 793
Fragments arranged by size:
856 793 427 234 162 147 99 87
33 21 14 13
NgoMIV G'CCGG_C
Cuts at: 0 451 1365 1473 2016 2344 2886
Size: 451 914 108 543 328 542
Fragments arranged by size:
914 543 542 451 328 108
NotI GC'GGCC_GC
Cuts at: 0 1508 1785 1970 2886
Size: 1508 277 185 916
Fragments arranged by size:
1508 916 277 185
NruI TCG'CGA
Cuts at: 0 1195 1283 1801 2886
Size: 1195 88 518 1085
Fragments arranged by size:
1195 1085 518 88
NspI r_CATG'y
Cuts at: 0 2871 2886
Size: 2871 15
PasI CC'CwG_GG
Cuts at: 0 1360 2886
Size: 1360 1526
PfoI T'CCnGG_A
Cuts at: 0 1337 2824 2886
Size: 1337 1487 62
Fragments arranged by size:
1487 1337 62
PmlI CAC'GTG
Cuts at: 0 564 2886
Size: 564 2322
PpuMI rG'GwC_Cy
Cuts at: 0 520 990 2507 2886
Size: 520 470 1517 379
Fragments arranged by size:
1517 520 470 379
PshAI GACnn'nnGTC
Cuts at: 0 518 2886
Size: 518 2368
PsiI TTA'TAA
Cuts at: 0 2881 2886
Size: 2881 5
PspOMI G'GGCC_C
Cuts at: 0 266 911 1077 1354 1521 2219 2886
Size: 266 645 166 277 167 698 667
Fragments arranged by size:
698 667 645 277 266 167 166
PstI C_TGCA'G
Cuts at: 0 45 374 639 669 938 2306 2436 2760
Size: 45 329 265 30 269 1368 130 324
Cuts at: 2760 2886
Size: 126
Fragments arranged by size:
1368 329 324 269 265 130 126 45
30
PvuII CAG'CTG
Cuts at: 0 2787 2886
Size: 2787 99
SacI G_AGCT'C
Cuts at: 0 463 1022 2886
Size: 463 559 1864
Fragments arranged by size:
1864 559 463
SacII CC_GC'GG
Cuts at: 0 1508 2886
Size: 1508 1378
SapI GCTCTTCn'nnn_
Cuts at: 0 818 2886
Size: 818 2068
SfcI C'TryA_G
Cuts at: 0 41 370 635 665 934 2302 2432 2756
Size: 41 329 265 30 269 1368 130 324
Cuts at: 2756 2886
Size: 130
Fragments arranged by size:
1368 329 324 269 265 130 130 41
30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1362 1939 2886
Size: 1362 577 947
Fragments arranged by size:
1362 947 577
SfoI GGC'GCC
Cuts at: 0 857 890 1124 1271 1370 1457 1478 1640
Size: 857 33 234 147 99 87 21 162
Cuts at: 1640 1653 1667 2094 2886
Size: 13 14 427 792
Fragments arranged by size:
857 792 427 234 162 147 99 87
33 21 14 13
SgrAI Cr'CCGG_yG
Cuts at: 0 885 1473 1644 2886
Size: 885 588 171 1242
Fragments arranged by size:
1242 885 588 171
SmaI CCC'GGG
Cuts at: 0 984 2351 2886
Size: 984 1367 535
Fragments arranged by size:
1367 984 535
SmlI C'TyrA_G
Cuts at: 0 996 2852 2886
Size: 996 1856 34
Fragments arranged by size:
1856 996 34
StuI AGG'CCT
Cuts at: 0 50 2886
Size: 50 2836
StyI C'CwwG_G
Cuts at: 0 51 1619 2232 2510 2582 2886
Size: 51 1568 613 278 72 304
Fragments arranged by size:
1568 613 304 278 72 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1435 1456 2538 2886
Size: 1435 21 1082 348
Fragments arranged by size:
1435 1082 348 21
TatI w'GTAC_w
Cuts at: 0 2809 2886
Size: 2809 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2164 2196 2886
Size: 2164 32 690
Fragments arranged by size:
2164 690 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1050 2886
Size: 15 1035 1836
Fragments arranged by size:
1836 1035 15
XmaI C'CCGG_G
Cuts at: 0 982 2349 2886
Size: 982 1367 537
Fragments arranged by size:
1367 982 537
XmnI GAAnn'nnTTC
Cuts at: 0 1717 2886
Size: 1717 1169
ZraI GAC'GTC
Cuts at: 0 1191 2886
Size: 1191 1695
Enzymes that do cut:
AatII Acc65I AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HindIII KasI KpnI MslI MspA1I
NaeI NarI NgoMIV NotI NruI NspI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SacII SapI SfcI SfiI SfoI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BmrI BmtI BsaBI
BsiWI BsmI BspHI BspMI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI EcoRI EcoRV FseI
FspAI HincII HpaI MfeI MluI MmeI MscI NcoI
NdeI NheI NsiI PacI PciI PflMI PmeI PpiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI