Restriction map for
pFN21AE2352
(Linear) (Six-base) MAPSORT of: KIEE2352.seq Check: 5721 from: 1 to: 933
KIEE2352.orf
With 182 enzymes: SgfI *
June 2, 2011 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 505 933
Size: 505 428
AccI GT'mk_AC
Cuts at: 0 216 933
Size: 216 717
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 933
Size: 159 774
AlwNI CAG_nnn'CTG
Cuts at: 0 412 933
Size: 412 521
ApoI r'AATT_y
Cuts at: 0 242 933
Size: 242 691
AvaI C'yCGr_G
Cuts at: 0 562 933
Size: 562 371
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 933
Size: 511 422
Bme1580I G_kGCm'C
Cuts at: 0 691 933
Size: 691 242
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 707 840 933
Size: 581 126 133 93
Fragments arranged by size:
581 133 126 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 333 933
Size: 42 291 600
Fragments arranged by size:
600 291 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 769 933
Size: 529 240 164
Fragments arranged by size:
529 240 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 90 333 933
Size: 90 243 600
Fragments arranged by size:
600 243 90
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 933
Size: 199 734
BseYI C'CCAG_C
Cuts at: 0 412 760 933
Size: 412 348 173
Fragments arranged by size:
412 348 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 933
Size: 298 635
BsiHKAI G_wGCw'C
Cuts at: 0 446 933
Size: 446 487
Bsp1286I G_dGCh'C
Cuts at: 0 446 691 933
Size: 446 245 242
Fragments arranged by size:
446 245 242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 505 933
Size: 505 428
BsrDI GCAATG_nn'
Cuts at: 0 186 933
Size: 186 747
BstZ17I GTA'TAC
Cuts at: 0 217 933
Size: 217 716
BtgI C'CryG_G
Cuts at: 0 579 848 933
Size: 579 269 85
Fragments arranged by size:
579 269 85
EaeI y'GGCC_r
Cuts at: 0 422 600 755 933
Size: 422 178 155 178
Fragments arranged by size:
422 178 178 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 529 769 933
Size: 159 370 240 164
Fragments arranged by size:
370 240 164 159
EcoNI CCTnn'n_nnAGG
Cuts at: 0 162 933
Size: 162 771
EcoRI G'AATT_C
Cuts at: 0 242 933
Size: 242 691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 717 721 749 933
Size: 689 28 4 28 184
Fragments arranged by size:
689 184 28 28 4
HindIII A'AGCT_T
Cuts at: 0 336 933
Size: 336 597
MscI TGG'CCA
Cuts at: 0 424 602 933
Size: 424 178 331
Fragments arranged by size:
424 331 178
MslI CAynn'nnrTG
Cuts at: 0 464 933
Size: 464 469
MspA1I CmG'CkG
Cuts at: 0 549 760 772 933
Size: 549 211 12 161
Fragments arranged by size:
549 211 161 12
NcoI C'CATG_G
Cuts at: 0 579 933
Size: 579 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 412 933
Size: 412 521
PfoI T'CCnGG_A
Cuts at: 0 67 933
Size: 67 866
PstI C_TGCA'G
Cuts at: 0 611 933
Size: 611 322
PvuII CAG'CTG
Cuts at: 0 772 933
Size: 772 161
SfcI C'TryA_G
Cuts at: 0 607 933
Size: 607 326
SmlI C'TyrA_G
Cuts at: 0 13 933
Size: 13 920
StuI AGG'CCT
Cuts at: 0 568 933
Size: 568 365
StyI C'CwwG_G
Cuts at: 0 579 933
Size: 579 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 218 642 933
Size: 218 424 291
Fragments arranged by size:
424 291 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 654 686 933
Size: 654 32 247
Fragments arranged by size:
654 247 32
Tth111I GACn'n_nGTC
Cuts at: 0 820 933
Size: 820 113
XbaI T'CTAG_A
Cuts at: 0 804 933
Size: 804 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 647 933
Size: 647 286
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AvaI BbsI Bme1580I
BmrI BplI BpmI BsaI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BstZ17I BtgI EaeI Eco57MI EcoNI
EcoRI Hin4I HindIII MscI MslI MspA1I NcoI PflMI
PfoI PstI PvuII SfcI SmlI StuI StyI TaqII
TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XhoI XmaI XmnI ZraI