(Linear) (Six-base) MAPSORT of: KIEE2324.seq  Check: 5799  from: 1  to: 999



KIEE2324.orf

 With 182 enzymes: SgfI * 

                        March 29, 2011 17:34  ..

AccI GT'mk_AC

Cuts at:      0      74     999 
   Size:         74     925
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599     656     845     999 
   Size:        599      57     189     154

  Fragments arranged by size: 

                 599     189     154      57
 
AflIII A'CryG_T

Cuts at:      0     985     999 
   Size:        985      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511     999 
   Size:        511     488
 
ApoI r'AATT_y

Cuts at:      0     271     786     999 
   Size:        271     515     213

  Fragments arranged by size: 

                 515     271     213
 
AvaI C'yCGr_G

Cuts at:      0      68     999 
   Size:         68     931
 
BclI T'GATC_A

Cuts at:      0     840     999 
   Size:        840     159
 
BfrBI ATG'CAT

Cuts at:      0     310     999 
   Size:        310     689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     999 
   Size:         41     958
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      17     999 
   Size:         17     982
 
BsaBI GATnn'nnATC

Cuts at:      0     257     999 
   Size:        257     742
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493     999 
   Size:        493     506
 
BsiHKAI G_wGCw'C

Cuts at:      0     977     999 
   Size:        977      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     977     999 
   Size:        977      22
 
BspHI T'CATG_A

Cuts at:      0     141     999 
   Size:        141     858
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     555     999 
   Size:        555     444
 
BsrDI GCAATG_nn'

Cuts at:      0     393     999 
   Size:        393     606
 
EaeI y'GGCC_r

Cuts at:      0      61     999 
   Size:         61     938
 
EarI CTCTTCn'nnn_

Cuts at:      0     632     698     853     999 
   Size:        632      66     155     146

  Fragments arranged by size: 

                 632     155     146      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     599     656     845     999 
   Size:         41     558      57     189     154

  Fragments arranged by size: 

                 558     189     154      57      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     536     999 
   Size:        536     463
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      14      46     999 
   Size:         14      32     953

  Fragments arranged by size: 

                 953      32      14
 
HincII GTy'rAC

Cuts at:      0     283     411     999 
   Size:        283     128     588

  Fragments arranged by size: 

                 588     283     128
 
MscI TGG'CCA

Cuts at:      0      63     999 
   Size:         63     936
 
NruI TCG'CGA

Cuts at:      0       8     999 
   Size:          8     991
 
NsiI A_TGCA'T

Cuts at:      0     312     999 
   Size:        312     687
 
NspI r_CATG'y

Cuts at:      0     989     999 
   Size:        989      10
 
PciI A'CATG_T

Cuts at:      0     985     999 
   Size:        985      14
 
PspXI vC'TCGA_Gb

Cuts at:      0      68     999 
   Size:         68     931
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      65      97     999 
   Size:         65      32     902

  Fragments arranged by size: 

                 902      65      32
 
PstI C_TGCA'G

Cuts at:      0     552     999 
   Size:        552     447
 
SfcI C'TryA_G

Cuts at:      0     548     554     994     999 
   Size:        548       6     440       5

  Fragments arranged by size: 

                 548     440       6       5
 
SmlI C'TyrA_G

Cuts at:      0      68     999 
   Size:         68     931
 
SpeI A'CTAG_T

Cuts at:      0     343     999 
   Size:        343     656
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     120     999 
   Size:        120     879
 
XhoI C'TCGA_G

Cuts at:      0      68     999 
   Size:         68     931
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AvaI     BclI    BfrBI
     BpmI     BsaI    BsaBI    BseRI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI     EaeI     EarI  Eco57MI    EcoNI    Hin4I   HincII     MscI
     NruI     NsiI     NspI     PciI    PspXI     PsrI     PstI     SfcI
     SmlI     SpeI    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI