Restriction map for
pFN21AE2323
(Linear) (Six-base) MAPSORT of: KIEE2323.seq Check: 9174 from: 1 to: 5124
KIEE2323.orf
With 182 enzymes: SgfI *
January 27, 2011 13:24 ..
AccI GT'mk_AC
Cuts at: 0 978 2627 4827 5124
Size: 978 1649 2200 297
Fragments arranged by size:
2200 1649 978 297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 571 827 1865 2324 4064 5124
Size: 49 522 256 1038 459 1740 1060
Fragments arranged by size:
1740 1060 1038 522 459 256 49
AfeI AGC'GCT
Cuts at: 0 317 5124
Size: 317 4807
AflII C'TTAA_G
Cuts at: 0 111 1561 1663 4149 5124
Size: 111 1450 102 2486 975
Fragments arranged by size:
2486 1450 975 111 102
AgeI A'CCGG_T
Cuts at: 0 3149 5124
Size: 3149 1975
AleI CACnn'nnGTG
Cuts at: 0 4818 5124
Size: 4818 306
AlwNI CAG_nnn'CTG
Cuts at: 0 2173 5071 5124
Size: 2173 2898 53
Fragments arranged by size:
2898 2173 53
ApoI r'AATT_y
Cuts at: 0 755 915 1828 2156 2233 3246 3297 3550
Size: 755 160 913 328 77 1013 51 253
Cuts at: 3550 3561 3846 4431 4479 4580 5124
Size: 11 285 585 48 101 544
Fragments arranged by size:
1013 913 755 585 544 328 285 253
160 101 77 51 48 11
AseI AT'TA_AT
Cuts at: 0 505 896 4193 4862 5124
Size: 505 391 3297 669 262
Fragments arranged by size:
3297 669 505 391 262
AvaI C'yCGr_G
Cuts at: 0 92 5124
Size: 92 5032
BamHI G'GATC_C
Cuts at: 0 923 5124
Size: 923 4201
BanI G'GyrC_C
Cuts at: 0 3861 5124
Size: 3861 1263
BanII G_rGCy'C
Cuts at: 0 2921 3410 3631 3700 5124
Size: 2921 489 221 69 1424
Fragments arranged by size:
2921 1424 489 221 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 830 1000 1465 1731 1927 2342 3515 4393
Size: 830 170 465 266 196 415 1173 878
Cuts at: 4393 4529 5124
Size: 136 595
Fragments arranged by size:
1173 878 830 595 465 415 266 196
170 136
BbvCI CC'TCA_GC
Cuts at: 0 1112 5124
Size: 1112 4012
BciVI GTATCCnnnnn_n'
Cuts at: 0 1560 4937 5124
Size: 1560 3377 187
Fragments arranged by size:
3377 1560 187
BclI T'GATC_A
Cuts at: 0 1248 5124
Size: 1248 3876
BfrBI ATG'CAT
Cuts at: 0 560 4023 4159 5055 5124
Size: 560 3463 136 896 69
Fragments arranged by size:
3463 896 560 136 69
BglII A'GATC_T
Cuts at: 0 1872 5124
Size: 1872 3252
BlpI GC'TnA_GC
Cuts at: 0 4752 5124
Size: 4752 372
BmgBI CAC'GTC
Cuts at: 0 2625 5124
Size: 2625 2499
BmrI ACTGGGnnnn_n'
Cuts at: 0 238 2976 3404 4910 5124
Size: 238 2738 428 1506 214
Fragments arranged by size:
2738 1506 428 238 214
BmtI G_CTAG'C
Cuts at: 0 4760 5124
Size: 4760 364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 5124
Size: 139 4985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 334 3851 4043 4436 5124
Size: 324 10 3517 192 393 688
Fragments arranged by size:
3517 688 393 324 192 10
Bpu10I CC'TnA_GC
Cuts at: 0 1112 1460 5124
Size: 1112 348 3664
Fragments arranged by size:
3664 1112 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3684 4727 5124
Size: 3684 1043 397
Fragments arranged by size:
3684 1043 397
BsaI GGTCTCn'nnnn_
Cuts at: 0 2821 5124
Size: 2821 2303
BsaAI yAC'GTr
Cuts at: 0 3646 5124
Size: 3646 1478
BsaBI GATnn'nnATC
Cuts at: 0 1529 3422 5124
Size: 1529 1893 1702
Fragments arranged by size:
1893 1702 1529
BsaHI Gr'CG_yC
Cuts at: 0 3811 5124
Size: 3811 1313
BsaWI w'CCGG_w
Cuts at: 0 2561 3149 5124
Size: 2561 588 1975
Fragments arranged by size:
2561 1975 588
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4409 4439 5124
Size: 4409 30 685
Fragments arranged by size:
4409 685 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 1045 5124
Size: 82 963 4079
Fragments arranged by size:
4079 963 82
BseYI C'CCAG_C
Cuts at: 0 1747 2270 4597 5124
Size: 1747 523 2327 527
Fragments arranged by size:
2327 1747 527 523
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 4971 5124
Size: 1430 3541 153
Fragments arranged by size:
3541 1430 153
BsiEI CG_ry'CG
Cuts at: 0 11 15 21 5124
Size: 11 4 6 5103
Fragments arranged by size:
5103 11 6 4
BsiHKAI G_wGCw'C
Cuts at: 0 3631 3700 3900 5124
Size: 3631 69 200 1224
Fragments arranged by size:
3631 1224 200 69
BsmI GAATG_Cn'
Cuts at: 0 474 4025 5124
Size: 474 3551 1099
Fragments arranged by size:
3551 1099 474
Bsp1286I G_dGCh'C
Cuts at: 0 2921 3410 3631 3700 3900 5124
Size: 2921 489 221 69 200 1224
Fragments arranged by size:
2921 1224 489 221 200 69
BspHI T'CATG_A
Cuts at: 0 4139 4893 5124
Size: 4139 754 231
Fragments arranged by size:
4139 754 231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 877 2639 4124 5124
Size: 877 1762 1485 1000
Fragments arranged by size:
1762 1485 1000 877
BsrBI CCG'CTC
Cuts at: 0 23 5124
Size: 23 5101
BsrDI GCAATG_nn'
Cuts at: 0 560 5124
Size: 560 4564
BsrFI r'CCGG_y
Cuts at: 0 3149 5124
Size: 3149 1975
BsrGI T'GTAC_A
Cuts at: 0 3456 5037 5124
Size: 3456 1581 87
Fragments arranged by size:
3456 1581 87
BssSI C'ACGA_G
Cuts at: 0 3710 5124
Size: 3710 1414
BstAPI GCAn_nnn'nTGC
Cuts at: 0 562 5124
Size: 562 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 855 2011 5124
Size: 855 1156 3113
Fragments arranged by size:
3113 1156 855
BstYI r'GATC_y
Cuts at: 0 84 923 1059 1144 1773 1872 5124
Size: 84 839 136 85 629 99 3252
Fragments arranged by size:
3252 839 629 136 99 85 84
BstZ17I GTA'TAC
Cuts at: 0 4828 5124
Size: 4828 296
BtgI C'CryG_G
Cuts at: 0 15 4232 5124
Size: 15 4217 892
Fragments arranged by size:
4217 892 15
BtsI GCAGTG_nn'
Cuts at: 0 1338 2043 3342 4734 4983 5124
Size: 1338 705 1299 1392 249 141
Fragments arranged by size:
1392 1338 1299 705 249 141
ClaI AT'CG_AT
Cuts at: 0 647 5124
Size: 647 4477
DraI TTT'AAA
Cuts at: 0 1427 5124
Size: 1427 3697
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 3816 5003 5124
Size: 547 3269 1187 121
Fragments arranged by size:
3269 1187 547 121
EaeI y'GGCC_r
Cuts at: 0 12 18 134 210 1669 3001 5124
Size: 12 6 116 76 1459 1332 2123
Fragments arranged by size:
2123 1459 1332 116 76 12 6
EagI C'GGCC_G
Cuts at: 0 12 18 5124
Size: 12 6 5106
Fragments arranged by size:
5106 12 6
EarI CTCTTCn'nnn_
Cuts at: 0 1841 3888 5124
Size: 1841 2047 1236
Fragments arranged by size:
2047 1841 1236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 5124
Size: 66 5058
EcoICRI GAG'CTC
Cuts at: 0 3629 3698 5124
Size: 3629 69 1426
Fragments arranged by size:
3629 1426 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 324 334 571 827 1865 2324 3851
Size: 49 275 10 237 256 1038 459 1527
Cuts at: 3851 4043 4064 4436 5124
Size: 192 21 372 688
Fragments arranged by size:
1527 1038 688 459 372 275 256 237
192 49 21 10
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1456 2897 5124
Size: 675 781 1441 2227
Fragments arranged by size:
2227 1441 781 675
EcoRI G'AATT_C
Cuts at: 0 2233 4479 5124
Size: 2233 2246 645
Fragments arranged by size:
2246 2233 645
EcoRV GAT'ATC
Cuts at: 0 249 325 1380 2316 3189 5124
Size: 249 76 1055 936 873 1935
Fragments arranged by size:
1935 1055 936 873 249 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1645 2794 5124
Size: 1645 1149 2330
Fragments arranged by size:
2330 1645 1149
HaeII r_GCGC'y
Cuts at: 0 319 5124
Size: 319 4805
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 915 947 1240 1256 1272 1288
Size: 314 32 569 32 293 16 16 16
Cuts at: 1288 2970 3002 3687 3719 4723 4755 5124
Size: 1682 32 685 32 1004 32 369
Fragments arranged by size:
1682 1004 685 569 369 314 293 32
32 32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 0 1969 2520 3083 5124
Size: 1969 551 563 2041
Fragments arranged by size:
2041 1969 563 551
HindIII A'AGCT_T
Cuts at: 0 193 4046 5124
Size: 193 3853 1078
Fragments arranged by size:
3853 1078 193
MfeI C'AATT_G
Cuts at: 0 1502 2479 5124
Size: 1502 977 2645
Fragments arranged by size:
2645 1502 977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1063 2575 2666 4987 5124
Size: 495 568 1512 91 2321 137
Fragments arranged by size:
2321 1512 568 495 137 91
MscI TGG'CCA
Cuts at: 0 136 212 1671 3003 5124
Size: 136 76 1459 1332 2121
Fragments arranged by size:
2121 1459 1332 136 76
MslI CAynn'nnrTG
Cuts at: 0 2009 2365 2645 3101 3431 4093 4493 4547
Size: 2009 356 280 456 330 662 400 54
Cuts at: 4547 4818 5124
Size: 271 306
Fragments arranged by size:
2009 662 456 400 356 330 306 280
271 54
MspA1I CmG'CkG
Cuts at: 0 17 62 81 805 1111 5124
Size: 17 45 19 724 306 4013
Fragments arranged by size:
4013 724 306 45 19 17
NcoI C'CATG_G
Cuts at: 0 4232 5124
Size: 4232 892
NdeI CA'TA_TG
Cuts at: 0 1295 3635 4251 4948 5124
Size: 1295 2340 616 697 176
Fragments arranged by size:
2340 1295 697 616 176
NheI G'CTAG_C
Cuts at: 0 4756 5124
Size: 4756 368
NotI GC'GGCC_GC
Cuts at: 0 18 5124
Size: 18 5106
NsiI A_TGCA'T
Cuts at: 0 562 4025 4161 5057 5124
Size: 562 3463 136 896 67
Fragments arranged by size:
3463 896 562 136 67
NspI r_CATG'y
Cuts at: 0 560 2333 5124
Size: 560 1773 2791
Fragments arranged by size:
2791 1773 560
PacI TTA_AT'TAA
Cuts at: 0 1589 5124
Size: 1589 3535
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 2685 3532 5124
Size: 132 2553 847 1592
Fragments arranged by size:
2553 1592 847 132
PpuMI rG'GwC_Cy
Cuts at: 0 1456 2897 5124
Size: 1456 1441 2227
Fragments arranged by size:
2227 1456 1441
PstI C_TGCA'G
Cuts at: 0 2169 2185 5124
Size: 2169 16 2939
Fragments arranged by size:
2939 2169 16
PvuI CG_AT'CG
Cuts at: 0 11 5124
Size: 11 5113
PvuII CAG'CTG
Cuts at: 0 805 1111 5124
Size: 805 306 4013
Fragments arranged by size:
4013 805 306
SacI G_AGCT'C
Cuts at: 0 3631 3700 5124
Size: 3631 69 1424
Fragments arranged by size:
3631 1424 69
SacII CC_GC'GG
Cuts at: 0 18 5124
Size: 18 5106
SapI GCTCTTCn'nnn_
Cuts at: 0 3888 5124
Size: 3888 1236
ScaI AGT'ACT
Cuts at: 0 944 3768 3780 5124
Size: 944 2824 12 1344
Fragments arranged by size:
2824 1344 944 12
SfcI C'TryA_G
Cuts at: 0 783 2118 2165 2181 2827 4006 4534 5099
Size: 783 1335 47 16 646 1179 528 565
Cuts at: 5099 5124
Size: 25
Fragments arranged by size:
1335 1179 783 646 565 528 47 25
16
SmlI C'TyrA_G
Cuts at: 0 111 1561 1663 3699 4149 4742 5124
Size: 111 1450 102 2036 450 593 382
Fragments arranged by size:
2036 1450 593 450 382 111 102
SnaBI TAC'GTA
Cuts at: 0 3646 5124
Size: 3646 1478
SpeI A'CTAG_T
Cuts at: 0 2655 3169 5124
Size: 2655 514 1955
Fragments arranged by size:
2655 1955 514
SphI G_CATG'C
Cuts at: 0 560 5124
Size: 560 4564
SspI AAT'ATT
Cuts at: 0 2700 5124
Size: 2700 2424
StyI C'CwwG_G
Cuts at: 0 1385 1787 2388 2786 4232 5124
Size: 1385 402 601 398 1446 892
Fragments arranged by size:
1446 1385 892 601 402 398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3236 3459 5124
Size: 3236 223 1665
Fragments arranged by size:
3236 1665 223
TatI w'GTAC_w
Cuts at: 0 942 2658 2691 2910 3172 3456 3520 3766
Size: 942 1716 33 219 262 284 64 246
Cuts at: 3766 3778 4516 5037 5124
Size: 12 738 521 87
Fragments arranged by size:
1716 942 738 521 284 262 246 219
87 64 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1296 1328 5124
Size: 1296 32 3796
Fragments arranged by size:
3796 1296 32
Tth111I GACn'n_nGTC
Cuts at: 0 791 5124
Size: 791 4333
XbaI T'CTAG_A
Cuts at: 0 3091 3970 5124
Size: 3091 879 1154
Fragments arranged by size:
3091 1154 879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1773 4655 5124
Size: 1773 2882 469
Fragments arranged by size:
2882 1773 469
XmnI GAAnn'nnTTC
Cuts at: 0 1832 1995 4694 5027 5124
Size: 1832 163 2699 333 97
Fragments arranged by size:
2699 1832 333 163 97
Enzymes that do cut:
AccI AcuI AfeI AflII AgeI AleI AlwNI ApoI
AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BstZ17I BtgI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PacI PflMI PpuMI PstI PvuI PvuII SacI
SacII SapI ScaI SfcI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI
BglI Bme1580I BsiWI BsmBI BspEI BssHII BstBI BstEII
Bsu36I BtgZI CspCI DraIII EcoNI FseI FspI FspAI
HpaI KasI KpnI MluI NaeI NarI NgoMIV NruI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI RsrII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SrfI StuI SwaI
XhoI XmaI ZraI