Restriction map for
pFN21AE2315
(Linear) (Six-base) MAPSORT of: KIEE2315.seq Check: 7497 from: 1 to: 3303
KIEE2315.orf
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2014 3303
Size: 2014 1289
AccI GT'mk_AC
Cuts at: 0 1385 3303
Size: 1385 1918
AclI AA'CG_TT
Cuts at: 0 2789 3303
Size: 2789 514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2480 2652 3149 3303
Size: 2480 172 497 154
Fragments arranged by size:
2480 497 172 154
AflIII A'CryG_T
Cuts at: 0 317 3303
Size: 317 2986
AleI CACnn'nnGTG
Cuts at: 0 1931 1984 3303
Size: 1931 53 1319
Fragments arranged by size:
1931 1319 53
ApoI r'AATT_y
Cuts at: 0 612 1038 1059 1795 2080 2245 2866 3303
Size: 612 426 21 736 285 165 621 437
Fragments arranged by size:
736 621 612 437 426 285 165 21
AseI AT'TA_AT
Cuts at: 0 896 3028 3303
Size: 896 2132 275
Fragments arranged by size:
2132 896 275
AvaI C'yCGr_G
Cuts at: 0 100 2879 3303
Size: 100 2779 424
Fragments arranged by size:
2779 424 100
BamHI G'GATC_C
Cuts at: 0 1686 3303
Size: 1686 1617
BanII G_rGCy'C
Cuts at: 0 101 107 2064 2979 3303
Size: 101 6 1957 915 324
Fragments arranged by size:
1957 915 324 101 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1517 1639 3303
Size: 1517 122 1664
Fragments arranged by size:
1664 1517 122
BciVI GTATCCnnnnn_n'
Cuts at: 0 2167 3303
Size: 2167 1136
BclI T'GATC_A
Cuts at: 0 642 1692 3150 3303
Size: 642 1050 1458 153
Fragments arranged by size:
1458 1050 642 153
BmgBI CAC'GTC
Cuts at: 0 1645 2503 3303
Size: 1645 858 800
Fragments arranged by size:
1645 858 800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1992 3303
Size: 1992 1311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 3071 3303
Size: 581 2490 232
Fragments arranged by size:
2490 581 232
Bpu10I CC'TnA_GC
Cuts at: 0 2075 3303
Size: 2075 1228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 2974 3303
Size: 347 2627 329
Fragments arranged by size:
2627 347 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 284 965 2833 3303
Size: 284 681 1868 470
Fragments arranged by size:
1868 681 470 284
BsaBI GATnn'nnATC
Cuts at: 0 1691 3303
Size: 1691 1612
BsaHI Gr'CG_yC
Cuts at: 0 3165 3303
Size: 3165 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 774 804 3303
Size: 774 30 2499
Fragments arranged by size:
2499 774 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 142 1981 2962 2971 3303
Size: 139 3 1839 981 9 332
Fragments arranged by size:
1839 981 332 139 9 3
BseYI C'CCAG_C
Cuts at: 0 728 958 3303
Size: 728 230 2345
Fragments arranged by size:
2345 728 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1989 3107 3303
Size: 1989 1118 196
Fragments arranged by size:
1989 1118 196
BsiEI CG_ry'CG
Cuts at: 0 95 3303
Size: 95 3208
BsiHKAI G_wGCw'C
Cuts at: 0 101 1991 2064 3137 3303
Size: 101 1890 73 1073 166
Fragments arranged by size:
1890 1073 166 101 73
Bsp1286I G_dGCh'C
Cuts at: 0 101 107 1991 2064 2979 3137 3303
Size: 101 6 1884 73 915 158 166
Fragments arranged by size:
1884 915 166 158 101 73 6
BspHI T'CATG_A
Cuts at: 0 2327 3303
Size: 2327 976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2011 2014 3079 3303
Size: 2011 3 1065 224
Fragments arranged by size:
2011 1065 224 3
BsrDI GCAATG_nn'
Cuts at: 0 1878 3303
Size: 1878 1425
BsrFI r'CCGG_y
Cuts at: 0 26 177 3303
Size: 26 151 3126
Fragments arranged by size:
3126 151 26
BsrGI T'GTAC_A
Cuts at: 0 2549 3303
Size: 2549 754
BssSI C'ACGA_G
Cuts at: 0 1099 3303
Size: 1099 2204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 3303
Size: 1128 2175
BstYI r'GATC_y
Cuts at: 0 1686 3303
Size: 1686 1617
BtgI C'CryG_G
Cuts at: 0 89 164 1416 3303
Size: 89 75 1252 1887
Fragments arranged by size:
1887 1252 89 75
DraI TTT'AAA
Cuts at: 0 1895 2087 3303
Size: 1895 192 1216
Fragments arranged by size:
1895 1216 192
EaeI y'GGCC_r
Cuts at: 0 92 3303
Size: 92 3211
EagI C'GGCC_G
Cuts at: 0 92 3303
Size: 92 3211
EarI CTCTTCn'nnn_
Cuts at: 0 630 753 2040 2459 3227 3236 3303
Size: 630 123 1287 419 768 9 67
Fragments arranged by size:
1287 768 630 419 123 67 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 13 171 3303
Size: 13 158 3132
Fragments arranged by size:
3132 158 13
EcoICRI GAG'CTC
Cuts at: 0 99 2062 3303
Size: 99 1963 1241
Fragments arranged by size:
1963 1241 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 2480 2652 3071 3149 3303
Size: 581 1899 172 419 78 154
Fragments arranged by size:
1899 581 419 172 154 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 3303
Size: 886 2417
EcoRI G'AATT_C
Cuts at: 0 1038 2245 3303
Size: 1038 1207 1058
Fragments arranged by size:
1207 1058 1038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1147 3275 3303
Size: 1147 2128 28
Fragments arranged by size:
2128 1147 28
HaeII r_GCGC'y
Cuts at: 0 83 3303
Size: 83 3220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 730 762 910 942 3135 3142
Size: 655 32 43 32 148 32 2193 7
Cuts at: 3142 3167 3174 3303
Size: 25 7 129
Fragments arranged by size:
2193 655 148 129 43 32 32 32
25 7 7
HincII GTy'rAC
Cuts at: 0 369 1611 2031 2796 3303
Size: 369 1242 420 765 507
Fragments arranged by size:
1242 765 507 420 369
HindIII A'AGCT_T
Cuts at: 0 60 2733 3303
Size: 60 2673 570
Fragments arranged by size:
2673 570 60
MfeI C'AATT_G
Cuts at: 0 1175 3146 3303
Size: 1175 1971 157
Fragments arranged by size:
1971 1175 157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1294 3303
Size: 1120 174 2009
Fragments arranged by size:
2009 1120 174
MslI CAynn'nnrTG
Cuts at: 0 1931 1984 2942 3303
Size: 1931 53 958 361
Fragments arranged by size:
1931 958 361 53
MspA1I CmG'CkG
Cuts at: 0 91 166 3303
Size: 91 75 3137
Fragments arranged by size:
3137 91 75
NaeI GCC'GGC
Cuts at: 0 28 3303
Size: 28 3275
NdeI CA'TA_TG
Cuts at: 0 1167 3303
Size: 1167 2136
NgoMIV G'CCGG_C
Cuts at: 0 26 3303
Size: 26 3277
NspI r_CATG'y
Cuts at: 0 270 321 418 3303
Size: 270 51 97 2885
Fragments arranged by size:
2885 270 97 51
PciI A'CATG_T
Cuts at: 0 317 3303
Size: 317 2986
PflMI CCAn_nnn'nTGG
Cuts at: 0 1084 2849 3303
Size: 1084 1765 454
Fragments arranged by size:
1765 1084 454
PshAI GACnn'nnGTC
Cuts at: 0 938 3303
Size: 938 2365
PsiI TTA'TAA
Cuts at: 0 1658 2453 3303
Size: 1658 795 850
Fragments arranged by size:
1658 850 795
PspXI vC'TCGA_Gb
Cuts at: 0 100 2879 3303
Size: 100 2779 424
Fragments arranged by size:
2779 424 100
SacI G_AGCT'C
Cuts at: 0 101 2064 3303
Size: 101 1963 1239
Fragments arranged by size:
1963 1239 101
SacII CC_GC'GG
Cuts at: 0 92 167 3303
Size: 92 75 3136
Fragments arranged by size:
3136 92 75
SfcI C'TryA_G
Cuts at: 0 419 2522 3303
Size: 419 2103 781
Fragments arranged by size:
2103 781 419
SgrAI Cr'CCGG_yG
Cuts at: 0 26 3303
Size: 26 3277
SmlI C'TyrA_G
Cuts at: 0 100 362 2879 2953 3303
Size: 100 262 2517 74 350
Fragments arranged by size:
2517 350 262 100 74
SpeI A'CTAG_T
Cuts at: 0 763 3303
Size: 763 2540
SspI AAT'ATT
Cuts at: 0 848 1540 1866 3303
Size: 848 692 326 1437
Fragments arranged by size:
1437 848 692 326
StuI AGG'CCT
Cuts at: 0 1972 3303
Size: 1972 1331
StyI C'CwwG_G
Cuts at: 0 228 936 2252 3303
Size: 228 708 1316 1051
Fragments arranged by size:
1316 1051 708 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 3303
Size: 628 2675
TatI w'GTAC_w
Cuts at: 0 2549 3303
Size: 2549 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2762 2794 3303
Size: 2762 32 509
Fragments arranged by size:
2762 509 32
XbaI T'CTAG_A
Cuts at: 0 853 3303
Size: 853 2450
XhoI C'TCGA_G
Cuts at: 0 100 2879 3303
Size: 100 2779 424
Fragments arranged by size:
2779 424 100
XmnI GAAnn'nnTTC
Cuts at: 0 765 1721 2594 3303
Size: 765 956 873 709
Fragments arranged by size:
956 873 765 709
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI ApoI AseI
AvaI BamHI BanII BbsI BciVI BclI BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI BtgI DraI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I
NaeI NdeI NgoMIV NspI PciI PflMI PshAI PsiI
PspXI SacI SacII SfcI SgrAI SmlI SpeI SspI
StuI StyI TaqII TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BsaAI BsaWI BsiWI BsmI BsmBI BspEI
BsrBI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII DrdI EcoO109I EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI MscI NarI
NcoI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SnaBI SphI SrfI SwaI
Tth111I XcmI XmaI ZraI