Restriction map for
pFN21AE2314
(Linear) (Six-base) MAPSORT of: KIEE2314.seq Check: 2811 from: 1 to: 6144
KIEE2314.orf
With 182 enzymes: SgfI *
January 31, 2011 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5666 6144
Size: 5666 478
AccI GT'mk_AC
Cuts at: 0 511 5272 6144
Size: 511 4761 872
Fragments arranged by size:
4761 872 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 833 1262 1790 4577 5354 6144
Size: 383 450 429 528 2787 777 790
Fragments arranged by size:
2787 790 777 528 450 429 383
AflII C'TTAA_G
Cuts at: 0 4072 6144
Size: 4072 2072
AflIII A'CryG_T
Cuts at: 0 1267 2725 6144
Size: 1267 1458 3419
Fragments arranged by size:
3419 1458 1267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3124 6144
Size: 3124 3020
AlwNI CAG_nnn'CTG
Cuts at: 0 3721 5658 5807 6144
Size: 3721 1937 149 337
Fragments arranged by size:
3721 1937 337 149
ApaLI G'TGCA_C
Cuts at: 0 4970 6144
Size: 4970 1174
ApoI r'AATT_y
Cuts at: 0 1324 2050 2180 2646 3054 3129 3141 3153
Size: 1324 726 130 466 408 75 12 12
Cuts at: 3153 3276 3324 3861 4131 4153 4416 5115 5208
Size: 123 48 537 270 22 263 699 93
Cuts at: 5208 5320 5792 6144
Size: 112 472 352
Fragments arranged by size:
1324 726 699 537 472 466 408 352
270 263 130 123 112 93 75 48
22 12 12
AseI AT'TA_AT
Cuts at: 0 2735 6144
Size: 2735 3409
AvaI C'yCGr_G
Cuts at: 0 296 1430 6144
Size: 296 1134 4714
Fragments arranged by size:
4714 1134 296
BanI G'GyrC_C
Cuts at: 0 117 238 6144
Size: 117 121 5906
Fragments arranged by size:
5906 121 117
BanII G_rGCy'C
Cuts at: 0 40 103 1699 6144
Size: 40 63 1596 4445
Fragments arranged by size:
4445 1596 63 40
BbeI G_GCGC'C
Cuts at: 0 121 242 6144
Size: 121 121 5902
Fragments arranged by size:
5902 121 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 1226 2681 4451 6144
Size: 405 821 1455 1770 1693
Fragments arranged by size:
1770 1693 1455 821 405
BbvCI CC'TCA_GC
Cuts at: 0 6056 6144
Size: 6056 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 487 5211 6144
Size: 487 4724 933
Fragments arranged by size:
4724 933 487
BclI T'GATC_A
Cuts at: 0 2247 3384 3612 6144
Size: 2247 1137 228 2532
Fragments arranged by size:
2532 2247 1137 228
BglII A'GATC_T
Cuts at: 0 6062 6144
Size: 6062 82
BlpI GC'TnA_GC
Cuts at: 0 143 191 6144
Size: 143 48 5953
Fragments arranged by size:
5953 143 48
Bme1580I G_kGCm'C
Cuts at: 0 4974 6144
Size: 4974 1170
BmgBI CAC'GTC
Cuts at: 0 32 4933 6144
Size: 32 4901 1211
Fragments arranged by size:
4901 1211 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 352 3364 6144
Size: 262 90 3012 2780
Fragments arranged by size:
3012 2780 262 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 806 1597 5902 6144
Size: 169 637 791 4305 242
Fragments arranged by size:
4305 791 637 242 169
Bpu10I CC'TnA_GC
Cuts at: 0 3035 6056 6144
Size: 3035 3021 88
Fragments arranged by size:
3035 3021 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 2102 2147 3070 6144
Size: 24 2078 45 923 3074
Fragments arranged by size:
3074 2078 923 45 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1420 3522 5603 6144
Size: 1420 2102 2081 541
Fragments arranged by size:
2102 2081 1420 541
BsaAI yAC'GTr
Cuts at: 0 1264 1270 6144
Size: 1264 6 4874
Fragments arranged by size:
4874 1264 6
BsaBI GATnn'nnATC
Cuts at: 0 475 2979 6144
Size: 475 2504 3165
Fragments arranged by size:
3165 2504 475
BsaHI Gr'CG_yC
Cuts at: 0 118 239 6144
Size: 118 121 5905
Fragments arranged by size:
5905 121 118
BsaWI w'CCGG_w
Cuts at: 0 79 337 6144
Size: 79 258 5807
Fragments arranged by size:
5807 258 79
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 614 2981 4846 5038 5041 6073 6144
Size: 614 2367 1865 192 3 1032 71
Fragments arranged by size:
2367 1865 1032 614 192 71 3
BseYI C'CCAG_C
Cuts at: 0 5407 6144
Size: 5407 737
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 271 564 3029 3095 3302 3959 4955
Size: 210 61 293 2465 66 207 657 996
Cuts at: 4955 6144
Size: 1189
Fragments arranged by size:
2465 1189 996 657 293 210 207 66
61
BsiHKAI G_wGCw'C
Cuts at: 0 4974 6144
Size: 4974 1170
BsiWI C'GTAC_G
Cuts at: 0 182 6144
Size: 182 5962
BsmI GAATG_Cn'
Cuts at: 0 1398 2326 4473 6144
Size: 1398 928 2147 1671
Fragments arranged by size:
2147 1671 1398 928
Bsp1286I G_dGCh'C
Cuts at: 0 40 103 1699 4974 6144
Size: 40 63 1596 3275 1170
Fragments arranged by size:
3275 1596 1170 63 40
BspEI T'CCGG_A
Cuts at: 0 79 6144
Size: 79 6065
BspHI T'CATG_A
Cuts at: 0 843 1226 3345 3648 5472 6144
Size: 843 383 2119 303 1824 672
Fragments arranged by size:
2119 1824 843 672 383 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 791 1839 5666 6144
Size: 791 1048 3827 478
Fragments arranged by size:
3827 1048 791 478
BsrBI CCG'CTC
Cuts at: 0 5 195 6144
Size: 5 190 5949
Fragments arranged by size:
5949 190 5
BsrDI GCAATG_nn'
Cuts at: 0 2110 6144
Size: 2110 4034
BsrGI T'GTAC_A
Cuts at: 0 374 1710 3252 3624 3921 6144
Size: 374 1336 1542 372 297 2223
Fragments arranged by size:
2223 1542 1336 374 372 297
BssSI C'ACGA_G
Cuts at: 0 3608 6144
Size: 3608 2536
BstBI TT'CG_AA
Cuts at: 0 2888 3826 6144
Size: 2888 938 2318
Fragments arranged by size:
2888 2318 938
BstYI r'GATC_y
Cuts at: 0 5131 5648 6062 6144
Size: 5131 517 414 82
Fragments arranged by size:
5131 517 414 82
Bsu36I CC'TnA_GG
Cuts at: 0 572 1536 4313 6144
Size: 572 964 2777 1831
Fragments arranged by size:
2777 1831 964 572
BtgI C'CryG_G
Cuts at: 0 233 365 6144
Size: 233 132 5779
Fragments arranged by size:
5779 233 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5186 6144
Size: 5186 958
BtsI GCAGTG_nn'
Cuts at: 0 4185 6144
Size: 4185 1959
ClaI AT'CG_AT
Cuts at: 0 2259 6144
Size: 2259 3885
DraI TTT'AAA
Cuts at: 0 2055 2558 3603 4304 4637 5114 5213 6144
Size: 2055 503 1045 701 333 477 99 931
Fragments arranged by size:
2055 1045 931 701 503 477 333 99
DraIII CAC_nnn'GTG
Cuts at: 0 1933 3937 6144
Size: 1933 2004 2207
Fragments arranged by size:
2207 2004 1933
EaeI y'GGCC_r
Cuts at: 0 494 1209 1527 1731 6144
Size: 494 715 318 204 4413
Fragments arranged by size:
4413 715 494 318 204
EarI CTCTTCn'nnn_
Cuts at: 0 1452 1687 2306 2888 4470 4823 6144
Size: 1452 235 619 582 1582 353 1321
Fragments arranged by size:
1582 1452 1321 619 582 353 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2984 6144
Size: 2984 3160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 383 806 833 1262 1597 1790 4577
Size: 169 214 423 27 429 335 193 2787
Cuts at: 4577 5354 5902 6144
Size: 777 548 242
Fragments arranged by size:
2787 777 548 429 423 335 242 214
193 169 27
EcoO109I rG'GnC_Cy
Cuts at: 0 1851 6144
Size: 1851 4293
EcoRI G'AATT_C
Cuts at: 0 2180 3129 3153 4131 5792 6144
Size: 2180 949 24 978 1661 352
Fragments arranged by size:
2180 1661 978 949 352 24
EcoRV GAT'ATC
Cuts at: 0 975 6144
Size: 975 5169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 1684 2611 2914 3488 4567 6144
Size: 429 1255 927 303 574 1079 1577
Fragments arranged by size:
1577 1255 1079 927 574 429 303
FspI TGC'GCA
Cuts at: 0 5141 5435 6144
Size: 5141 294 709
Fragments arranged by size:
5141 709 294
HaeII r_GCGC'y
Cuts at: 0 121 242 5783 6144
Size: 121 121 5541 361
Fragments arranged by size:
5541 361 121 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 825 857 1424 1456 2356 2388
Size: 680 32 113 32 567 32 900 32
Cuts at: 2388 2842 2874 4488 4520 6144
Size: 454 32 1614 32 1624
Fragments arranged by size:
1624 1614 900 680 567 454 113 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 5070 6144
Size: 5070 1074
HindIII A'AGCT_T
Cuts at: 0 518 862 6144
Size: 518 344 5282
Fragments arranged by size:
5282 518 344
HpaI GTT'AAC
Cuts at: 0 5070 6144
Size: 5070 1074
KasI G'GCGC_C
Cuts at: 0 117 238 6144
Size: 117 121 5906
Fragments arranged by size:
5906 121 117
MfeI C'AATT_G
Cuts at: 0 261 6144
Size: 261 5883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1326 1832 3312 3530 5908 6144
Size: 134 1192 506 1480 218 2378 236
Fragments arranged by size:
2378 1480 1192 506 236 218 134
MslI CAynn'nnrTG
Cuts at: 0 1109 3162 5584 6097 6144
Size: 1109 2053 2422 513 47
Fragments arranged by size:
2422 2053 1109 513 47
MspA1I CmG'CkG
Cuts at: 0 136 147 235 285 389 613 3124 6144
Size: 136 11 88 50 104 224 2511 3020
Fragments arranged by size:
3020 2511 224 136 104 88 50 11
NarI GG'CG_CC
Cuts at: 0 118 239 6144
Size: 118 121 5905
Fragments arranged by size:
5905 121 118
NspI r_CATG'y
Cuts at: 0 2729 4519 6144
Size: 2729 1790 1625
Fragments arranged by size:
2729 1790 1625
PciI A'CATG_T
Cuts at: 0 2725 6144
Size: 2725 3419
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 921 6144
Size: 471 450 5223
Fragments arranged by size:
5223 471 450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1448 1480 6144
Size: 1448 32 4664
Fragments arranged by size:
4664 1448 32
PpuMI rG'GwC_Cy
Cuts at: 0 1851 6144
Size: 1851 4293
PshAI GACnn'nnGTC
Cuts at: 0 1522 6144
Size: 1522 4622
PsiI TTA'TAA
Cuts at: 0 1672 1793 2452 2837 2930 6144
Size: 1672 121 659 385 93 3214
Fragments arranged by size:
3214 1672 659 385 121 93
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4714 4746 6144
Size: 4714 32 1398
Fragments arranged by size:
4714 1398 32
PstI C_TGCA'G
Cuts at: 0 5663 6144
Size: 5663 481
PvuII CAG'CTG
Cuts at: 0 147 285 3124 6144
Size: 147 138 2839 3020
Fragments arranged by size:
3020 2839 147 138
SacII CC_GC'GG
Cuts at: 0 236 6144
Size: 236 5908
SapI GCTCTTCn'nnn_
Cuts at: 0 1687 2888 6144
Size: 1687 1201 3256
Fragments arranged by size:
3256 1687 1201
ScaI AGT'ACT
Cuts at: 0 1275 6144
Size: 1275 4869
SexAI A'CCwGG_T
Cuts at: 0 464 741 6144
Size: 464 277 5403
Fragments arranged by size:
5403 464 277
SfcI C'TryA_G
Cuts at: 0 1992 3708 5313 5659 6066 6144
Size: 1992 1716 1605 346 407 78
Fragments arranged by size:
1992 1716 1605 407 346 78
SfoI GGC'GCC
Cuts at: 0 119 240 6144
Size: 119 121 5904
Fragments arranged by size:
5904 121 119
SmaI CCC'GGG
Cuts at: 0 298 6144
Size: 298 5846
SmlI C'TyrA_G
Cuts at: 0 39 2117 2162 3049 4072 6144
Size: 39 2078 45 887 1023 2072
Fragments arranged by size:
2078 2072 1023 887 45 39
SpeI A'CTAG_T
Cuts at: 0 693 2800 3868 6144
Size: 693 2107 1068 2276
Fragments arranged by size:
2276 2107 1068 693
SspI AAT'ATT
Cuts at: 0 1044 2664 3219 3734 5061 6144
Size: 1044 1620 555 515 1327 1083
Fragments arranged by size:
1620 1327 1083 1044 555 515
StuI AGG'CCT
Cuts at: 0 2279 6144
Size: 2279 3865
StyI C'CwwG_G
Cuts at: 0 113 434 5147 6144
Size: 113 321 4713 997
Fragments arranged by size:
4713 997 321 113
SwaI ATTT'AAAT
Cuts at: 0 2055 6144
Size: 2055 4089
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1624 6144
Size: 1624 4520
TatI w'GTAC_w
Cuts at: 0 374 1273 1710 2728 3252 3624 3921 4012
Size: 374 899 437 1018 524 372 297 91
Cuts at: 4012 4730 6144
Size: 718 1414
Fragments arranged by size:
1414 1018 899 718 524 437 374 372
297 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 6144
Size: 526 32 5586
Fragments arranged by size:
5586 526 32
Tth111I GACn'n_nGTC
Cuts at: 0 507 1191 4502 6144
Size: 507 684 3311 1642
Fragments arranged by size:
3311 1642 684 507
XbaI T'CTAG_A
Cuts at: 0 207 595 1245 3765 4161 6144
Size: 207 388 650 2520 396 1983
Fragments arranged by size:
2520 1983 650 396 388 207
XmaI C'CCGG_G
Cuts at: 0 296 6144
Size: 296 5848
XmnI GAAnn'nnTTC
Cuts at: 0 5635 6144
Size: 5635 509
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AlwNI ApaLI
ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstBI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MslI
MspA1I NarI NspI PciI PflMI PpiI PpuMI PshAI
PsiI PsrI PstI PvuII SacII SapI ScaI SexAI
SfcI SfoI SmaI SmlI SpeI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaI AscI AsiSI AvrII BaeI BamHI BcgI BfrBI
BglI BmtI BplI BsaXI BsiEI BsmBI BsrFI BssHII
BstAPI BstEII BstXI BstZ17I CspCI DrdI EagI EcoICRI
EcoNI FseI FspAI KpnI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PspOMI PspXI PvuI RsrII SacI
SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SphI
SrfI XcmI XhoI ZraI