Restriction map for
pFN21AE2312
(Linear) (Six-base) MAPSORT of: KIEE2312.seq Check: 5562 from: 1 to: 6408
KIEE2312.orf
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3575 6408
Size: 3575 2833
AccI GT'mk_AC
Cuts at: 0 647 4016 4166 6408
Size: 647 3369 150 2242
Fragments arranged by size:
3369 2242 647 150
AclI AA'CG_TT
Cuts at: 0 3179 5916 6408
Size: 3179 2737 492
Fragments arranged by size:
3179 2737 492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1369 2341 3034 3613 4267 4890 5038 5478
Size: 1369 972 693 579 654 623 148 440
Cuts at: 5478 5899 5974 6408
Size: 421 75 434
Fragments arranged by size:
1369 972 693 654 623 579 440 434
421 148 75
AflIII A'CryG_T
Cuts at: 0 1995 2219 4034 5981 6408
Size: 1995 224 1815 1947 427
Fragments arranged by size:
1995 1947 1815 427 224
AhdI GACnn_n'nnGTC
Cuts at: 0 5352 6408
Size: 5352 1056
AleI CACnn'nnGTG
Cuts at: 0 2494 6408
Size: 2494 3914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1358 4348 5729 6408
Size: 1358 2990 1381 679
Fragments arranged by size:
2990 1381 1358 679
AlwNI CAG_nnn'CTG
Cuts at: 0 1224 2163 2296 2556 3012 5376 6148 6295
Size: 1224 939 133 260 456 2364 772 147
Cuts at: 6295 6408
Size: 113
Fragments arranged by size:
2364 1224 939 772 456 260 147 133
113
ApaI G_GGCC'C
Cuts at: 0 4050 6408
Size: 4050 2358
ApaLI G'TGCA_C
Cuts at: 0 1007 3562 6408
Size: 1007 2555 2846
Fragments arranged by size:
2846 2555 1007
ApoI r'AATT_y
Cuts at: 0 3438 3970 6361 6408
Size: 3438 532 2391 47
Fragments arranged by size:
3438 2391 532 47
AvaI C'yCGr_G
Cuts at: 0 326 779 1768 6408
Size: 326 453 989 4640
Fragments arranged by size:
4640 989 453 326
AvrII C'CTAG_G
Cuts at: 0 5442 6408
Size: 5442 966
BamHI G'GATC_C
Cuts at: 0 1297 1448 6408
Size: 1297 151 4960
Fragments arranged by size:
4960 1297 151
BanI G'GyrC_C
Cuts at: 0 145 1585 2488 3129 3860 5430 5582 6298
Size: 145 1440 903 641 731 1570 152 716
Cuts at: 6298 6408
Size: 110
Fragments arranged by size:
1570 1440 903 731 716 641 152 145
110
BanII G_rGCy'C
Cuts at: 0 2266 4050 4114 4871 5125 5492 6408
Size: 2266 1784 64 757 254 367 916
Fragments arranged by size:
2266 1784 916 757 367 254 64
BbeI G_GCGC'C
Cuts at: 0 5434 6408
Size: 5434 974
BbsI GAAGACnn'nnnn_
Cuts at: 0 647 886 1531 2577 2929 3042 3520 4072
Size: 647 239 645 1046 352 113 478 552
Cuts at: 4072 5597 6408
Size: 1525 811
Fragments arranged by size:
1525 1046 811 647 645 552 478 352
239 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 39 73 799 833 3333 3367 5391 5425
Size: 39 34 726 34 2500 34 2024 34
Cuts at: 5425 6408
Size: 983
Fragments arranged by size:
2500 2024 983 726 39 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4986 6408
Size: 4986 1422
BfrBI ATG'CAT
Cuts at: 0 793 5196 6408
Size: 793 4403 1212
Fragments arranged by size:
4403 1212 793
BglII A'GATC_T
Cuts at: 0 1167 2862 6408
Size: 1167 1695 3546
Fragments arranged by size:
3546 1695 1167
BlpI GC'TnA_GC
Cuts at: 0 2265 2297 4870 5806 6408
Size: 2265 32 2573 936 602
Fragments arranged by size:
2573 2265 936 602 32
Bme1580I G_kGCm'C
Cuts at: 0 148 1011 3315 3566 4050 6303 6408
Size: 148 863 2304 251 484 2253 105
Fragments arranged by size:
2304 2253 863 484 251 148 105
BmgBI CAC'GTC
Cuts at: 0 2563 5049 5427 5664 6408
Size: 2563 2486 378 237 744
Fragments arranged by size:
2563 2486 744 378 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 1718 2539 2571 2959 3158 4992 6408
Size: 1718 821 32 388 199 1834 1416
Fragments arranged by size:
1834 1718 1416 821 388 199 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5562 6408
Size: 5562 846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 3124 3142 4822 5407 6142 6408
Size: 160 2964 18 1680 585 735 266
Fragments arranged by size:
2964 1680 735 585 266 160 18
Bpu10I CC'TnA_GC
Cuts at: 0 4911 6408
Size: 4911 1497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 2812 3706 4810 6408
Size: 1498 1314 894 1104 1598
Fragments arranged by size:
1598 1498 1314 1104 894
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 1954 2020 3829 4225 6408
Size: 325 1629 66 1809 396 2183
Fragments arranged by size:
2183 1809 1629 396 325 66
BsaAI yAC'GTr
Cuts at: 0 385 1996 6408
Size: 385 1611 4412
Fragments arranged by size:
4412 1611 385
BsaBI GATnn'nnATC
Cuts at: 0 1490 6408
Size: 1490 4918
BsaHI Gr'CG_yC
Cuts at: 0 4887 5431 6408
Size: 4887 544 977
Fragments arranged by size:
4887 977 544
BsaWI w'CCGG_w
Cuts at: 0 86 3395 6408
Size: 86 3309 3013
Fragments arranged by size:
3309 3013 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2614 2644 4417 4447 6408
Size: 2614 30 1773 30 1961
Fragments arranged by size:
2614 1961 1773 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 2635 3106 3109 3709 4422 6145 6408
Size: 640 1995 471 3 600 713 1723 263
Fragments arranged by size:
1995 1723 713 640 600 471 263 3
BseYI C'CCAG_C
Cuts at: 0 480 1417 1519 1817 2556 2757 3839 4254
Size: 480 937 102 298 739 201 1082 415
Cuts at: 4254 4509 4952 6288 6408
Size: 255 443 1336 120
Fragments arranged by size:
1336 1082 937 739 480 443 415 298
255 201 120 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 696 1663 1746 2450 3013 3685 4097
Size: 628 68 967 83 704 563 672 412
Cuts at: 4097 4354 4558 4758 4760 6136 6408
Size: 257 204 200 2 1376 272
Fragments arranged by size:
1376 967 704 672 628 563 412 272
257 204 200 83 68 2
BsiHKAI G_wGCw'C
Cuts at: 0 62 1011 1509 2538 3566 3749 4114 5492
Size: 62 949 498 1029 1028 183 365 1378
Cuts at: 5492 6408
Size: 916
Fragments arranged by size:
1378 1029 1028 949 916 498 365 183
62
BsmI GAATG_Cn'
Cuts at: 0 2739 2789 4103 4424 6408
Size: 2739 50 1314 321 1984
Fragments arranged by size:
2739 1984 1314 321 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1474 4697 6408
Size: 1474 3223 1711
Fragments arranged by size:
3223 1711 1474
Bsp1286I G_dGCh'C
Cuts at: 0 62 148 1011 1509 2266 2538 3315 3566
Size: 62 86 863 498 757 272 777 251
Cuts at: 3566 3749 4050 4114 4871 5125 5492 6303 6408
Size: 183 301 64 757 254 367 811 105
Fragments arranged by size:
863 811 777 757 757 498 367 301
272 254 251 183 105 86 64 62
BspEI T'CCGG_A
Cuts at: 0 3395 6408
Size: 3395 3013
BspHI T'CATG_A
Cuts at: 0 1089 2108 2817 6323 6408
Size: 1089 1019 709 3506 85
Fragments arranged by size:
3506 1089 1019 709 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 1209 3575 3917 4152 4865 5324 5684
Size: 482 727 2366 342 235 713 459 360
Cuts at: 5684 6408
Size: 724
Fragments arranged by size:
2366 727 724 713 482 459 360 342
235
BsrBI CCG'CTC
Cuts at: 0 4446 6408
Size: 4446 1962
BsrDI GCAATG_nn'
Cuts at: 0 1619 5161 5802 6152 6408
Size: 1619 3542 641 350 256
Fragments arranged by size:
3542 1619 641 350 256
BsrFI r'CCGG_y
Cuts at: 0 230 3266 6408
Size: 230 3036 3142
Fragments arranged by size:
3142 3036 230
BsrGI T'GTAC_A
Cuts at: 0 2498 4130 6408
Size: 2498 1632 2278
Fragments arranged by size:
2498 2278 1632
BssSI C'ACGA_G
Cuts at: 0 2386 6408
Size: 2386 4022
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3117 3918 4084 6408
Size: 3117 801 166 2324
Fragments arranged by size:
3117 2324 801 166
BstEII G'GTnAC_C
Cuts at: 0 5844 6408
Size: 5844 564
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1596 4497 6408
Size: 1596 2901 1911
Fragments arranged by size:
2901 1911 1596
BstYI r'GATC_y
Cuts at: 0 1107 1167 1182 1297 1448 2325 2394 2835
Size: 1107 60 15 115 151 877 69 441
Cuts at: 2835 2862 4785 5015 6408
Size: 27 1923 230 1393
Fragments arranged by size:
1923 1393 1107 877 441 230 151 115
69 60 27 15
BstZ17I GTA'TAC
Cuts at: 0 648 4017 4167 6408
Size: 648 3369 150 2241
Fragments arranged by size:
3369 2241 648 150
Bsu36I CC'TnA_GG
Cuts at: 0 738 3407 5602 6408
Size: 738 2669 2195 806
Fragments arranged by size:
2669 2195 806 738
BtgI C'CryG_G
Cuts at: 0 1178 1213 4052 4142 4622 4877 6408
Size: 1178 35 2839 90 480 255 1531
Fragments arranged by size:
2839 1531 1178 480 255 90 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4326 6408
Size: 4326 2082
BtsI GCAGTG_nn'
Cuts at: 0 2308 4524 4712 4770 6096 6408
Size: 2308 2216 188 58 1326 312
Fragments arranged by size:
2308 2216 1326 312 188 58
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5839 5874 6408
Size: 5839 35 534
Fragments arranged by size:
5839 534 35
DraIII CAC_nnn'GTG
Cuts at: 0 1015 6408
Size: 1015 5393
DrdI GACnn_nn'nnGTC
Cuts at: 0 700 1946 6408
Size: 700 1246 4462
Fragments arranged by size:
4462 1246 700
EaeI y'GGCC_r
Cuts at: 0 884 1001 1268 1676 1895 3264 4625 6408
Size: 884 117 267 408 219 1369 1361 1783
Fragments arranged by size:
1783 1369 1361 884 408 267 219 117
EarI CTCTTCn'nnn_
Cuts at: 0 195 659 1322 1727 4354 4479 4909 5497
Size: 195 464 663 405 2627 125 430 588
Cuts at: 5497 6169 6176 6408
Size: 672 7 232
Fragments arranged by size:
2627 672 663 588 464 430 405 232
195 125 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 6408
Size: 19 6389
EcoICRI GAG'CTC
Cuts at: 0 4112 5490 6408
Size: 4112 1378 918
Fragments arranged by size:
4112 1378 918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1369 2341 3034 3124 3142 3613 4267
Size: 160 1209 972 693 90 18 471 654
Cuts at: 4267 4822 4890 5038 5407 5478 5899 5974 6142
Size: 555 68 148 369 71 421 75 168
Cuts at: 6142 6408
Size: 266
Fragments arranged by size:
1209 972 693 654 555 471 421 369
266 168 160 148 90 75 71 68
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2230 4861 5749 6408
Size: 2230 2631 888 659
Fragments arranged by size:
2631 2230 888 659
EcoO109I rG'GnC_Cy
Cuts at: 0 1127 1236 1873 4046 4047 4149 5622 6145
Size: 1127 109 637 2173 1 102 1473 523
Cuts at: 6145 6408
Size: 263
Fragments arranged by size:
2173 1473 1127 637 523 263 109 102
1
EcoRV GAT'ATC
Cuts at: 0 390 6408
Size: 390 6018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2034 5154 5166 6294 6408
Size: 2034 3120 12 1128 114
Fragments arranged by size:
3120 2034 1128 114 12
HaeII r_GCGC'y
Cuts at: 0 1674 2856 4125 5103 5434 6408
Size: 1674 1182 1269 978 331 974
Fragments arranged by size:
1674 1269 1182 978 974 331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1111 1143 1862 1894 5338 5370
Size: 106 32 973 32 719 32 3444 32
Cuts at: 5370 5519 5551 5710 5742 6199 6231 6408
Size: 149 32 159 32 457 32 177
Fragments arranged by size:
3444 973 719 457 177 159 149 106
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2001 2376 6408
Size: 2001 375 4032
Fragments arranged by size:
4032 2001 375
HindIII A'AGCT_T
Cuts at: 0 1886 2686 6408
Size: 1886 800 3722
Fragments arranged by size:
3722 1886 800
KasI G'GCGC_C
Cuts at: 0 5430 6408
Size: 5430 978
MluI A'CGCG_T
Cuts at: 0 5981 6408
Size: 5981 427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1962 2500 3530 3916 4776 5324 6254
Size: 251 1711 538 1030 386 860 548 930
Cuts at: 6254 6408
Size: 154
Fragments arranged by size:
1711 1030 930 860 548 538 386 251
154
MscI TGG'CCA
Cuts at: 0 1003 1270 1678 1897 4627 6408
Size: 1003 267 408 219 2730 1781
Fragments arranged by size:
2730 1781 1003 408 267 219
MslI CAynn'nnrTG
Cuts at: 0 151 955 1576 1594 1918 2494 3082 3527
Size: 151 804 621 18 324 576 588 445
Cuts at: 3527 3805 4141 4547 4963 5024 5207 5959 6408
Size: 278 336 406 416 61 183 752 449
Fragments arranged by size:
804 752 621 588 576 449 445 416
406 336 324 278 183 151 61 18
MspA1I CmG'CkG
Cuts at: 0 406 807 915 1722 1821 2878 3009 3207
Size: 406 401 108 807 99 1057 131 198
Cuts at: 3207 3783 3821 3843 4061 5709 5886 5994 6120
Size: 576 38 22 218 1648 177 108 126
Cuts at: 6120 6408
Size: 288
Fragments arranged by size:
1648 1057 807 576 406 401 288 218
198 177 131 126 108 108 99 38
22
NarI GG'CG_CC
Cuts at: 0 5431 6408
Size: 5431 977
NcoI C'CATG_G
Cuts at: 0 1178 4142 4622 4877 6408
Size: 1178 2964 480 255 1531
Fragments arranged by size:
2964 1531 1178 480 255
NdeI CA'TA_TG
Cuts at: 0 2064 6408
Size: 2064 4344
NsiI A_TGCA'T
Cuts at: 0 795 5198 6408
Size: 795 4403 1210
Fragments arranged by size:
4403 1210 795
NspI r_CATG'y
Cuts at: 0 1339 1551 2146 2223 2700 3366 3501 5502
Size: 1339 212 595 77 477 666 135 2001
Cuts at: 5502 5706 6408
Size: 204 702
Fragments arranged by size:
2001 1339 702 666 595 477 212 204
135 77
PciI A'CATG_T
Cuts at: 0 2219 6408
Size: 2219 4189
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 1266 4509 4929 6295 6408
Size: 570 696 3243 420 1366 113
Fragments arranged by size:
3243 1366 696 570 420 113
PfoI T'CCnGG_A
Cuts at: 0 1129 2179 2213 4190 6408
Size: 1129 1050 34 1977 2218
Fragments arranged by size:
2218 1977 1129 1050 34
PmlI CAC'GTG
Cuts at: 0 385 6408
Size: 385 6023
PpuMI rG'GwC_Cy
Cuts at: 0 1127 1236 1873 5622 6408
Size: 1127 109 637 3749 786
Fragments arranged by size:
3749 1127 786 637 109
PshAI GACnn'nnGTC
Cuts at: 0 1871 6408
Size: 1871 4537
PspOMI G'GGCC_C
Cuts at: 0 4046 6408
Size: 4046 2362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4171 4203 5290 5322 6408
Size: 4171 32 1087 32 1086
Fragments arranged by size:
4171 1087 1086 32 32
PstI C_TGCA'G
Cuts at: 0 806 2552 3113 3572 3914 4109 4519 5321
Size: 806 1746 561 459 342 195 410 802
Cuts at: 5321 5687 6408
Size: 366 721
Fragments arranged by size:
1746 806 802 721 561 459 410 366
342 195
PvuII CAG'CTG
Cuts at: 0 406 915 1722 1821 2878 3009 3207 3843
Size: 406 509 807 99 1057 131 198 636
Cuts at: 3843 5709 5886 5994 6120 6408
Size: 1866 177 108 126 288
Fragments arranged by size:
1866 1057 807 636 509 406 288 198
177 131 126 108 99
SacI G_AGCT'C
Cuts at: 0 4114 5492 6408
Size: 4114 1378 916
Fragments arranged by size:
4114 1378 916
SapI GCTCTTCn'nnn_
Cuts at: 0 195 4479 4909 5497 6408
Size: 195 4284 430 588 911
Fragments arranged by size:
4284 911 588 430 195
SbfI CC_TGCA'GG
Cuts at: 0 5321 5687 6408
Size: 5321 366 721
Fragments arranged by size:
5321 721 366
ScaI AGT'ACT
Cuts at: 0 2632 3796 5002 6408
Size: 2632 1164 1206 1406
Fragments arranged by size:
2632 1406 1206 1164
SexAI A'CCwGG_T
Cuts at: 0 1238 4976 6408
Size: 1238 3738 1432
Fragments arranged by size:
3738 1432 1238
SfcI C'TryA_G
Cuts at: 0 802 2548 2626 3109 3568 3910 4105 4515
Size: 802 1746 78 483 459 342 195 410
Cuts at: 4515 5317 5683 6408
Size: 802 366 725
Fragments arranged by size:
1746 802 802 725 483 459 410 366
342 195 78
SfoI GGC'GCC
Cuts at: 0 5432 6408
Size: 5432 976
SmlI C'TyrA_G
Cuts at: 0 1477 2827 3721 4825 6408
Size: 1477 1350 894 1104 1583
Fragments arranged by size:
1583 1477 1350 1104 894
SphI G_CATG'C
Cuts at: 0 1551 3501 5706 6408
Size: 1551 1950 2205 702
Fragments arranged by size:
2205 1950 1551 702
StuI AGG'CCT
Cuts at: 0 5773 6408
Size: 5773 635
StyI C'CwwG_G
Cuts at: 0 1178 1251 1637 2009 2588 2738 4142 4490
Size: 1178 73 386 372 579 150 1404 348
Cuts at: 4490 4565 4622 4877 5442 5519 5864 6263 6408
Size: 75 57 255 565 77 345 399 145
Fragments arranged by size:
1404 1178 579 565 399 386 372 348
345 255 150 145 77 75 73 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1831 2393 2506 5789 5863 6183 6408
Size: 1831 562 113 3283 74 320 225
Fragments arranged by size:
3283 1831 562 320 225 113 74
TatI w'GTAC_w
Cuts at: 0 35 2498 2630 3794 4130 5000 6317 6356
Size: 35 2463 132 1164 336 870 1317 39
Cuts at: 6356 6408
Size: 52
Fragments arranged by size:
2463 1317 1164 870 336 132 52 39
35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 406 6408
Size: 374 32 6002
Fragments arranged by size:
6002 374 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 1635 3594 3623 4149 4458 5005 6270
Size: 367 1268 1959 29 526 309 547 1265
Cuts at: 6270 6408
Size: 138
Fragments arranged by size:
1959 1268 1265 547 526 367 309 138
29
XmnI GAAnn'nnTTC
Cuts at: 0 3439 5134 6408
Size: 3439 1695 1274
Fragments arranged by size:
3439 1695 1274
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII KasI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NsiI NspI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SapI SbfI ScaI SexAI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AloI AscI AseI
AsiSI BaeI BbvCI BclI BglI BmtI BsiEI BsiWI
BssHII BstBI ClaI DraI EagI EcoRI FseI FspI
FspAI HpaI KpnI MfeI NaeI NgoMIV NheI NotI
NruI PacI PasI PmeI PpiI PsiI PspXI PvuI
RsrII SacII SalI SanDI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI