Restriction map for
pFN21AE2309
(Linear) (Six-base) MAPSORT of: KIEE2309.seq Check: 372 from: 1 to: 3771
KIEE2309.orf
With 182 enzymes: SgfI *
July 3, 2012 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1253 1648 2268 3771
Size: 1253 395 620 1503
Fragments arranged by size:
1503 1253 620 395
AccI GT'mk_AC
Cuts at: 0 3143 3771
Size: 3143 628
Acc65I G'GTAC_C
Cuts at: 0 488 3771
Size: 488 3283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1394 1849 2558 2612 3771
Size: 1394 455 709 54 1159
Fragments arranged by size:
1394 1159 709 455 54
AflIII A'CryG_T
Cuts at: 0 2336 3771
Size: 2336 1435
AhdI GACnn_n'nnGTC
Cuts at: 0 198 3771
Size: 198 3573
AleI CACnn'nnGTG
Cuts at: 0 811 3712 3771
Size: 811 2901 59
Fragments arranged by size:
2901 811 59
AlwNI CAG_nnn'CTG
Cuts at: 0 837 3457 3771
Size: 837 2620 314
Fragments arranged by size:
2620 837 314
AvaI C'yCGr_G
Cuts at: 0 108 298 595 1096 1548 1586 2048 3322
Size: 108 190 297 501 452 38 462 1274
Cuts at: 3322 3429 3771
Size: 107 342
Fragments arranged by size:
1274 501 462 452 342 297 190 108
107 38
BamHI G'GATC_C
Cuts at: 0 1582 1795 3771
Size: 1582 213 1976
Fragments arranged by size:
1976 1582 213
BanI G'GyrC_C
Cuts at: 0 403 488 1042 1076 1089 1331 1651 3663
Size: 403 85 554 34 13 242 320 2012
Cuts at: 3663 3771
Size: 108
Fragments arranged by size:
2012 554 403 320 242 108 85 34
13
BanII G_rGCy'C
Cuts at: 0 920 1014 1362 1483 1501 1519 1603 1618
Size: 920 94 348 121 18 18 84 15
Cuts at: 1618 2254 2419 2452 2642 3534 3567 3771
Size: 636 165 33 190 892 33 204
Fragments arranged by size:
920 892 636 348 204 190 165 121
94 84 33 33 18 18 15
BbeI G_GCGC'C
Cuts at: 0 407 1046 3771
Size: 407 639 2725
Fragments arranged by size:
2725 639 407
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 1175 1242 1361 3252 3771
Size: 45 1130 67 119 1891 519
Fragments arranged by size:
1891 1130 519 119 67 45
BbvCI CC'TCA_GC
Cuts at: 0 3345 3388 3771
Size: 3345 43 383
Fragments arranged by size:
3345 383 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1020 1054 3771
Size: 1020 34 2717
Fragments arranged by size:
2717 1020 34
BclI T'GATC_A
Cuts at: 0 440 3771
Size: 440 3331
BfrBI ATG'CAT
Cuts at: 0 960 3771
Size: 960 2811
BglI GCCn_nnn'nGGC
Cuts at: 0 376 617 658 1254 2109 3771
Size: 376 241 41 596 855 1662
Fragments arranged by size:
1662 855 596 376 241 41
BglII A'GATC_T
Cuts at: 0 2928 3771
Size: 2928 843
BlpI GC'TnA_GC
Cuts at: 0 580 748 1305 3771
Size: 580 168 557 2466
Fragments arranged by size:
2466 580 557 168
Bme1580I G_kGCm'C
Cuts at: 0 275 737 1654 1667 3771
Size: 275 462 917 13 2104
Fragments arranged by size:
2104 917 462 275 13
BmgBI CAC'GTC
Cuts at: 0 1324 3736 3771
Size: 1324 2412 35
Fragments arranged by size:
2412 1324 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 2553 3771
Size: 2553 1218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1129 2322 3771
Size: 1129 1193 1449
Fragments arranged by size:
1449 1193 1129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1518 1712 1748 1940 2124 2372 2621
Size: 1209 309 194 36 192 184 248 249
Cuts at: 2621 3581 3771
Size: 960 190
Fragments arranged by size:
1209 960 309 249 248 194 192 190
184 36
Bpu10I CC'TnA_GC
Cuts at: 0 838 1482 1500 1617 1707 1743 1779 3345
Size: 838 644 18 117 90 36 36 1566
Cuts at: 3345 3388 3771
Size: 43 383
Fragments arranged by size:
1566 838 644 383 117 90 43 36
36 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2798 3771
Size: 2798 973
BsaI GGTCTCn'nnnn_
Cuts at: 0 1680 2655 3323 3771
Size: 1680 975 668 448
Fragments arranged by size:
1680 975 668 448
BsaAI yAC'GTr
Cuts at: 0 2100 2950 3714 3771
Size: 2100 850 764 57
Fragments arranged by size:
2100 850 764 57
BsaHI Gr'CG_yC
Cuts at: 0 311 404 1043 3249 3771
Size: 311 93 639 2206 522
Fragments arranged by size:
2206 639 522 311 93
BsaWI w'CCGG_w
Cuts at: 0 3636 3771
Size: 3636 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 2787 2817 3771
Size: 775 30 1982 30 954
Fragments arranged by size:
1982 954 775 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 796 1889 1892 1997 2153 3771
Size: 223 573 1093 3 105 156 1618
Fragments arranged by size:
1618 1093 573 223 156 105 3
BseYI C'CCAG_C
Cuts at: 0 315 729 1204 1312 1317 3184 3771
Size: 315 414 475 108 5 1867 587
Fragments arranged by size:
1867 587 475 414 315 108 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1016 1225 2063 3771
Size: 71 945 209 838 1708
Fragments arranged by size:
1708 945 838 209 71
BsiEI CG_ry'CG
Cuts at: 0 122 395 653 3771
Size: 122 273 258 3118
Fragments arranged by size:
3118 273 258 122
BsiHKAI G_wGCw'C
Cuts at: 0 293 920 1362 1636 2419 2452 3771
Size: 293 627 442 274 783 33 1319
Fragments arranged by size:
1319 783 627 442 293 274 33
BsmI GAATG_Cn'
Cuts at: 0 2002 3771
Size: 2002 1769
Bsp1286I G_dGCh'C
Cuts at: 0 275 293 737 920 1014 1362 1483 1501
Size: 275 18 444 183 94 348 121 18
Cuts at: 1501 1519 1603 1618 1636 1654 1667 2254 2419
Size: 18 84 15 18 18 13 587 165
Cuts at: 2419 2452 2642 3534 3567 3771
Size: 33 190 892 33 204
Fragments arranged by size:
892 587 444 348 275 204 190 183
165 121 94 84 33 33 18 18
18 18 18 15 13
BspEI T'CCGG_A
Cuts at: 0 3636 3771
Size: 3636 135
BspHI T'CATG_A
Cuts at: 0 2634 3771
Size: 2634 1137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 260 696 881 918 1250 1253 1648
Size: 224 36 436 185 37 332 3 395
Cuts at: 1648 2268 3771
Size: 620 1503
Fragments arranged by size:
1503 620 436 395 332 224 185 37
36 3
BsrBI CCG'CTC
Cuts at: 0 420 752 1095 1273 3634 3771
Size: 420 332 343 178 2361 137
Fragments arranged by size:
2361 420 343 332 178 137
BsrDI GCAATG_nn'
Cuts at: 0 500 3027 3077 3771
Size: 500 2527 50 694
Fragments arranged by size:
2527 694 500 50
BsrFI r'CCGG_y
Cuts at: 0 648 1284 2356 3544 3771
Size: 648 636 1072 1188 227
Fragments arranged by size:
1188 1072 648 636 227
BssHII G'CGCG_C
Cuts at: 0 629 3771
Size: 629 3142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3586 3771
Size: 3586 185
BstEII G'GTnAC_C
Cuts at: 0 87 3771
Size: 87 3684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 1690 1726 2831 3771
Size: 729 961 36 1105 940
Fragments arranged by size:
1105 961 940 729 36
BstYI r'GATC_y
Cuts at: 0 1582 1795 1837 2030 2928 3771
Size: 1582 213 42 193 898 843
Fragments arranged by size:
1582 898 843 213 193 42
Bsu36I CC'TnA_GG
Cuts at: 0 1147 2387 3771
Size: 1147 1240 1384
Fragments arranged by size:
1384 1240 1147
BtgI C'CryG_G
Cuts at: 0 170 371 430 1737 3083 3771
Size: 170 201 59 1307 1346 688
Fragments arranged by size:
1346 1307 688 201 170 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 547 3771
Size: 547 3224
BtsI GCAGTG_nn'
Cuts at: 0 89 3771
Size: 89 3682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2112 2147 3771
Size: 2112 35 1624
Fragments arranged by size:
2112 1624 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 141 220 784 2530 3771
Size: 141 79 564 1746 1241
Fragments arranged by size:
1746 1241 564 141 79
EaeI y'GGCC_r
Cuts at: 0 173 392 539 650 815 1286 1491 1509
Size: 173 219 147 111 165 471 205 18
Cuts at: 1509 1524 1608 1644 1680 1716 1734 2120 2983
Size: 15 84 36 36 36 18 386 863
Cuts at: 2983 3771
Size: 788
Fragments arranged by size:
863 788 471 386 219 205 173 165
147 111 84 36 36 36 18 18
15
EagI C'GGCC_G
Cuts at: 0 392 650 3771
Size: 392 258 3121
Fragments arranged by size:
3121 392 258
EarI CTCTTCn'nnn_
Cuts at: 0 200 2403 3029 3227 3771
Size: 200 2203 626 198 544
Fragments arranged by size:
2203 626 544 200 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 3459 3771
Size: 691 2768 312
Fragments arranged by size:
2768 691 312
EcoICRI GAG'CTC
Cuts at: 0 918 1360 2417 2450 3771
Size: 918 442 1057 33 1321
Fragments arranged by size:
1321 1057 918 442 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1394 1518 1712 1748 1849 1940 2124
Size: 1209 185 124 194 36 101 91 184
Cuts at: 2124 2372 2558 2612 2621 3581 3771
Size: 248 186 54 9 960 190
Fragments arranged by size:
1209 960 248 194 190 186 185 184
124 101 91 54 36 9
EcoO109I rG'GnC_Cy
Cuts at: 0 213 1121 1723 2053 2517 2547 3771
Size: 213 908 602 330 464 30 1224
Fragments arranged by size:
1224 908 602 464 330 213 30
HaeII r_GCGC'y
Cuts at: 0 282 407 609 1046 1194 2193 3771
Size: 282 125 202 437 148 999 1578
Fragments arranged by size:
1578 999 437 282 202 148 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 535 567 831 863 2596 2628
Size: 184 32 319 32 264 32 1733 32
Cuts at: 2628 2684 2716 3281 3313 3457 3489 3771
Size: 56 32 565 32 144 32 282
Fragments arranged by size:
1733 565 319 282 264 184 144 56
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1055 1977 2766 3144 3771
Size: 1055 922 789 378 627
Fragments arranged by size:
1055 922 789 627 378
HpaI GTT'AAC
Cuts at: 0 1055 3771
Size: 1055 2716
KasI G'GCGC_C
Cuts at: 0 403 1042 3771
Size: 403 639 2729
Fragments arranged by size:
2729 639 403
KpnI G_GTAC'C
Cuts at: 0 492 3771
Size: 492 3279
MfeI C'AATT_G
Cuts at: 0 1473 3771
Size: 1473 2298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 2392 3334 3771
Size: 533 1859 942 437
Fragments arranged by size:
1859 942 533 437
MscI TGG'CCA
Cuts at: 0 817 1511 1526 1610 1646 1682 1718 1736
Size: 817 694 15 84 36 36 36 18
Cuts at: 1736 2122 2985 3771
Size: 386 863 786
Fragments arranged by size:
863 817 786 694 386 84 36 36
36 18 15
MslI CAynn'nnrTG
Cuts at: 0 811 1465 2233 2341 3712 3771
Size: 811 654 768 108 1371 59
Fragments arranged by size:
1371 811 768 654 108 59
MspA1I CmG'CkG
Cuts at: 0 58 67 373 388 432 614 670 1016
Size: 58 9 306 15 44 182 56 346
Cuts at: 1016 1918 3295 3454 3482 3622 3628 3771
Size: 902 1377 159 28 140 6 143
Fragments arranged by size:
1377 902 346 306 182 159 143 140
58 56 44 28 15 9 6
NaeI GCC'GGC
Cuts at: 0 650 1286 3771
Size: 650 636 2485
Fragments arranged by size:
2485 650 636
NarI GG'CG_CC
Cuts at: 0 404 1043 3771
Size: 404 639 2728
Fragments arranged by size:
2728 639 404
NcoI C'CATG_G
Cuts at: 0 1737 3083 3771
Size: 1737 1346 688
Fragments arranged by size:
1737 1346 688
NgoMIV G'CCGG_C
Cuts at: 0 648 1284 3771
Size: 648 636 2487
Fragments arranged by size:
2487 648 636
NotI GC'GGCC_GC
Cuts at: 0 392 3771
Size: 392 3379
NruI TCG'CGA
Cuts at: 0 23 532 3771
Size: 23 509 3239
Fragments arranged by size:
3239 509 23
NsiI A_TGCA'T
Cuts at: 0 962 3771
Size: 962 2809
NspI r_CATG'y
Cuts at: 0 960 1464 2340 3771
Size: 960 504 876 1431
Fragments arranged by size:
1431 960 876 504
PasI CC'CwG_GG
Cuts at: 0 722 1422 3771
Size: 722 700 2349
Fragments arranged by size:
2349 722 700
PciI A'CATG_T
Cuts at: 0 2336 3771
Size: 2336 1435
PflMI CCAn_nnn'nTGG
Cuts at: 0 1918 2411 3771
Size: 1918 493 1360
Fragments arranged by size:
1918 1360 493
PfoI T'CCnGG_A
Cuts at: 0 150 909 2349 3771
Size: 150 759 1440 1422
Fragments arranged by size:
1440 1422 759 150
PmlI CAC'GTG
Cuts at: 0 2950 3714 3771
Size: 2950 764 57
Fragments arranged by size:
2950 764 57
PpuMI rG'GwC_Cy
Cuts at: 0 213 1121 1723 2053 2547 3771
Size: 213 908 602 330 494 1224
Fragments arranged by size:
1224 908 602 494 330 213
PspXI vC'TCGA_Gb
Cuts at: 0 1096 2048 3771
Size: 1096 952 1723
Fragments arranged by size:
1723 1096 952
PstI C_TGCA'G
Cuts at: 0 384 1341 3227 3372 3627 3771
Size: 384 957 1886 145 255 144
Fragments arranged by size:
1886 957 384 255 145 144
PvuII CAG'CTG
Cuts at: 0 1918 3454 3628 3771
Size: 1918 1536 174 143
Fragments arranged by size:
1918 1536 174 143
RsrII CG'GwC_CG
Cuts at: 0 907 3771
Size: 907 2864
SacI G_AGCT'C
Cuts at: 0 920 1362 2419 2452 3771
Size: 920 442 1057 33 1319
Fragments arranged by size:
1319 1057 920 442 33
SacII CC_GC'GG
Cuts at: 0 374 433 3771
Size: 374 59 3338
Fragments arranged by size:
3338 374 59
SalI G'TCGA_C
Cuts at: 0 3142 3771
Size: 3142 629
SanDI GG'GwC_CC
Cuts at: 0 2547 3771
Size: 2547 1224
SapI GCTCTTCn'nnn_
Cuts at: 0 2403 3227 3771
Size: 2403 824 544
Fragments arranged by size:
2403 824 544
SfcI C'TryA_G
Cuts at: 0 380 1026 1337 3011 3209 3223 3368 3623
Size: 380 646 311 1674 198 14 145 255
Cuts at: 3623 3771
Size: 148
Fragments arranged by size:
1674 646 380 311 255 198 148 145
14
SfoI GGC'GCC
Cuts at: 0 405 1044 3771
Size: 405 639 2727
Fragments arranged by size:
2727 639 405
SmaI CCC'GGG
Cuts at: 0 3431 3771
Size: 3431 340
SmlI C'TyrA_G
Cuts at: 0 298 595 1096 2048 2813 3322 3771
Size: 298 297 501 952 765 509 449
Fragments arranged by size:
952 765 509 501 449 298 297
StyI C'CwwG_G
Cuts at: 0 1527 1737 3083 3771
Size: 1527 210 1346 688
Fragments arranged by size:
1527 1346 688 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 895 1218 1326 3771
Size: 529 366 323 108 2445
Fragments arranged by size:
2445 529 366 323 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2185 2217 3771
Size: 2185 32 1554
Fragments arranged by size:
2185 1554 32
Tth111I GACn'n_nGTC
Cuts at: 0 138 2761 3771
Size: 138 2623 1010
Fragments arranged by size:
2623 1010 138
XbaI T'CTAG_A
Cuts at: 0 1636 3771
Size: 1636 2135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 1519 1744 3771
Size: 1212 307 225 2027
Fragments arranged by size:
2027 1212 307 225
XhoI C'TCGA_G
Cuts at: 0 298 595 1096 2048 3322 3771
Size: 298 297 501 952 1274 449
Fragments arranged by size:
1274 952 501 449 298 297
XmaI C'CCGG_G
Cuts at: 0 3429 3771
Size: 3429 342
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlwNI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HpaI KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmlI
PpuMI PspXI PstI PvuII RsrII SacI SacII SalI
SanDI SapI SfcI SfoI SmaI SmlI StyI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BmtI BsaBI BsiWI BsmBI BsrGI BssSI BstBI BstZ17I
ClaI DraI DraIII EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII MluI NdeI NheI PacI PmeI
PpiI PshAI PsiI PspOMI PsrI PvuI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XmnI ZraI