Restriction map for
pFN21AE2296
(Linear) (Six-base) MAPSORT of: KIEE2296.seq Check: 139 from: 1 to: 3495
KIEE2296.orf
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3045 3495
Size: 3045 450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 520 1241 1642 1753 2105 2663 3115
Size: 304 216 721 401 111 352 558 452
Cuts at: 3115 3245 3452 3495
Size: 130 207 43
Fragments arranged by size:
721 558 452 401 352 304 216 207
130 111 43
AflIII A'CryG_T
Cuts at: 0 1401 3495
Size: 1401 2094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1093 3495
Size: 1093 2402
AlwNI CAG_nnn'CTG
Cuts at: 0 543 640 2025 2128 3093 3495
Size: 543 97 1385 103 965 402
Fragments arranged by size:
1385 965 543 402 103 97
ApaLI G'TGCA_C
Cuts at: 0 1978 3495
Size: 1978 1517
ApoI r'AATT_y
Cuts at: 0 1051 1821 2445 3351 3459 3495
Size: 1051 770 624 906 108 36
Fragments arranged by size:
1051 906 770 624 108 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1882 1915 3353 3386 3495
Size: 1882 33 1438 33 109
Fragments arranged by size:
1882 1438 109 33 33
BanI G'GyrC_C
Cuts at: 0 1368 3495
Size: 1368 2127
BanII G_rGCy'C
Cuts at: 0 132 317 1844 3495
Size: 132 185 1527 1651
Fragments arranged by size:
1651 1527 185 132
BbvCI CC'TCA_GC
Cuts at: 0 1247 3495
Size: 1247 2248
BciVI GTATCCnnnnn_n'
Cuts at: 0 3354 3495
Size: 3354 141
BclI T'GATC_A
Cuts at: 0 2301 3495
Size: 2301 1194
BglI GCCn_nnn'nGGC
Cuts at: 0 1839 3495
Size: 1839 1656
BglII A'GATC_T
Cuts at: 0 1650 2628 3495
Size: 1650 978 867
Fragments arranged by size:
1650 978 867
BlpI GC'TnA_GC
Cuts at: 0 2744 3495
Size: 2744 751
Bme1580I G_kGCm'C
Cuts at: 0 1661 1982 3495
Size: 1661 321 1513
Fragments arranged by size:
1661 1513 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 2083 3495
Size: 2083 1412
BmtI G_CTAG'C
Cuts at: 0 1039 3495
Size: 1039 2456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1900 1936 2254 2437 3495
Size: 85 1815 36 318 183 1058
Fragments arranged by size:
1815 1058 318 183 85 36
Bpu10I CC'TnA_GC
Cuts at: 0 1247 3495
Size: 1247 2248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 3495
Size: 1519 1976
BsaI GGTCTCn'nnnn_
Cuts at: 0 3070 3495
Size: 3070 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1873 1903 2650 2680 3495
Size: 1873 30 747 30 815
Fragments arranged by size:
1873 815 747 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1217 1220 3495
Size: 1217 3 2275
Fragments arranged by size:
2275 1217 3
BseYI C'CCAG_C
Cuts at: 0 3234 3495
Size: 3234 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 2038 3076 3495
Size: 745 1293 1038 419
Fragments arranged by size:
1293 1038 745 419
BsiHKAI G_wGCw'C
Cuts at: 0 132 1886 1982 3495
Size: 132 1754 96 1513
Fragments arranged by size:
1754 1513 132 96
Bsp1286I G_dGCh'C
Cuts at: 0 132 317 1661 1844 1886 1982 3495
Size: 132 185 1344 183 42 96 1513
Fragments arranged by size:
1513 1344 185 183 132 96 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3045 3495
Size: 3045 450
BsrDI GCAATG_nn'
Cuts at: 0 716 932 3495
Size: 716 216 2563
Fragments arranged by size:
2563 716 216
BssSI C'ACGA_G
Cuts at: 0 1646 3495
Size: 1646 1849
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2592 3495
Size: 2592 903
BstYI r'GATC_y
Cuts at: 0 1650 2628 3495
Size: 1650 978 867
Fragments arranged by size:
1650 978 867
Bsu36I CC'TnA_GG
Cuts at: 0 810 1364 3495
Size: 810 554 2131
Fragments arranged by size:
2131 810 554
BtgI C'CryG_G
Cuts at: 0 650 3080 3495
Size: 650 2430 415
Fragments arranged by size:
2430 650 415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 785 3495
Size: 785 2710
BtsI GCAGTG_nn'
Cuts at: 0 115 519 617 875 1569 1753 3206 3495
Size: 115 404 98 258 694 184 1453 289
Fragments arranged by size:
1453 694 404 289 258 184 115 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2927 2962 3495
Size: 2927 35 533
Fragments arranged by size:
2927 533 35
DraI TTT'AAA
Cuts at: 0 1112 3495
Size: 1112 2383
DraIII CAC_nnn'GTG
Cuts at: 0 158 667 1977 2029 3495
Size: 158 509 1310 52 1466
Fragments arranged by size:
1466 1310 509 158 52
EaeI y'GGCC_r
Cuts at: 0 2328 3495
Size: 2328 1167
EarI CTCTTCn'nnn_
Cuts at: 0 651 1238 3495
Size: 651 587 2257
Fragments arranged by size:
2257 651 587
EcoICRI GAG'CTC
Cuts at: 0 130 3495
Size: 130 3365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 304 520 1241 1642 1753 1900 1936
Size: 85 219 216 721 401 111 147 36
Cuts at: 1936 2105 2254 2437 2663 3115 3245 3452 3495
Size: 169 149 183 226 452 130 207 43
Fragments arranged by size:
721 452 401 226 219 216 207 183
169 149 147 130 111 85 43 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1259 3495
Size: 1259 2236
EcoO109I rG'GnC_Cy
Cuts at: 0 1704 3495
Size: 1704 1791
EcoRI G'AATT_C
Cuts at: 0 1051 3495
Size: 1051 2444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1188 1871 2650 3495
Size: 1188 683 779 845
Fragments arranged by size:
1188 845 779 683
FspI TGC'GCA
Cuts at: 0 2735 3495
Size: 2735 760
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 117 149 1374 1406 1873 1905
Size: 69 32 16 32 1225 32 467 32
Cuts at: 1905 2193 2225 2265 2297 2532 2564 2650 2682
Size: 288 32 40 32 235 32 86 32
Cuts at: 2682 3376 3408 3495
Size: 694 32 87
Fragments arranged by size:
1225 694 467 288 235 87 86 69
40 32 32 32 32 32 32 32
32 32 16
HincII GTy'rAC
Cuts at: 0 412 2725 3495
Size: 412 2313 770
Fragments arranged by size:
2313 770 412
HindIII A'AGCT_T
Cuts at: 0 1853 2675 3495
Size: 1853 822 820
Fragments arranged by size:
1853 822 820
MfeI C'AATT_G
Cuts at: 0 1207 3495
Size: 1207 2288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1850 3495
Size: 1850 1645
MscI TGG'CCA
Cuts at: 0 2330 3495
Size: 2330 1165
MslI CAynn'nnrTG
Cuts at: 0 662 721 746 1404 2768 2774 3495
Size: 662 59 25 658 1364 6 721
Fragments arranged by size:
1364 721 662 658 59 25 6
MspA1I CmG'CkG
Cuts at: 0 865 1878 3289 3495
Size: 865 1013 1411 206
Fragments arranged by size:
1411 1013 865 206
NcoI C'CATG_G
Cuts at: 0 3080 3495
Size: 3080 415
NheI G'CTAG_C
Cuts at: 0 1035 3495
Size: 1035 2460
NspI r_CATG'y
Cuts at: 0 1405 1833 2483 2595 2848 2971 3495
Size: 1405 428 650 112 253 123 524
Fragments arranged by size:
1405 650 524 428 253 123 112
PciI A'CATG_T
Cuts at: 0 1401 3495
Size: 1401 2094
PflMI CCAn_nnn'nTGG
Cuts at: 0 1555 3495
Size: 1555 1940
PfoI T'CCnGG_A
Cuts at: 0 674 3495
Size: 674 2821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 290 322 3495
Size: 290 32 3173
Fragments arranged by size:
3173 290 32
PshAI GACnn'nnGTC
Cuts at: 0 1521 3495
Size: 1521 1974
PstI C_TGCA'G
Cuts at: 0 514 870 1008 1479 3216 3495
Size: 514 356 138 471 1737 279
Fragments arranged by size:
1737 514 471 356 279 138
PvuII CAG'CTG
Cuts at: 0 865 1878 3289 3495
Size: 865 1013 1411 206
Fragments arranged by size:
1411 1013 865 206
SacI G_AGCT'C
Cuts at: 0 132 3495
Size: 132 3363
SexAI A'CCwGG_T
Cuts at: 0 1973 3495
Size: 1973 1522
SfcI C'TryA_G
Cuts at: 0 458 510 632 866 1004 1475 1573 2686
Size: 458 52 122 234 138 471 98 1113
Cuts at: 2686 3212 3495
Size: 526 283
Fragments arranged by size:
1113 526 471 458 283 234 138 122
98 52
SmlI C'TyrA_G
Cuts at: 0 1534 3495
Size: 1534 1961
SphI G_CATG'C
Cuts at: 0 2483 2848 3495
Size: 2483 365 647
Fragments arranged by size:
2483 647 365
SspI AAT'ATT
Cuts at: 0 697 2599 3495
Size: 697 1902 896
Fragments arranged by size:
1902 896 697
StuI AGG'CCT
Cuts at: 0 883 1437 3495
Size: 883 554 2058
Fragments arranged by size:
2058 883 554
StyI C'CwwG_G
Cuts at: 0 1846 1929 3080 3495
Size: 1846 83 1151 415
Fragments arranged by size:
1846 1151 415 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 812 1876 3495
Size: 812 1064 1619
Fragments arranged by size:
1619 1064 812
TatI w'GTAC_w
Cuts at: 0 2424 3424 3495
Size: 2424 1000 71
Fragments arranged by size:
2424 1000 71
Tth111I GACn'n_nGTC
Cuts at: 0 41 3495
Size: 41 3454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 2716 3495
Size: 352 2364 779
Fragments arranged by size:
2364 779 352
XmnI GAAnn'nnTTC
Cuts at: 0 2351 3495
Size: 2351 1144
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaLI ApoI BaeI
BanI BanII BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NheI NspI PciI PflMI PfoI
PpiI PshAI PstI PvuII SacI SexAI SfcI SmlI
SphI SspI StuI StyI TaqII TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BbsI BcgI BfrBI BmgBI BplI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I ClaI DrdI EagI EciI EcoRV FseI FspAI
HaeII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TstI XbaI
XhoI XmaI ZraI