Restriction map for
pFN21AE2293
(Linear) (Six-base) MAPSORT of: KIEE2293.seq Check: 7190 from: 1 to: 618
KIEE2293.orf
With 182 enzymes: SgfI *
March 22, 2012 16:01 ..
AfeI AGC'GCT
Cuts at: 0 29 618
Size: 29 589
ApaI G_GGCC'C
Cuts at: 0 11 618
Size: 11 607
ApaLI G'TGCA_C
Cuts at: 0 462 618
Size: 462 156
AscI GG'CGCG_CC
Cuts at: 0 166 618
Size: 166 452
AvaI C'yCGr_G
Cuts at: 0 10 618
Size: 10 608
BanI G'GyrC_C
Cuts at: 0 60 82 214 618
Size: 60 22 132 404
Fragments arranged by size:
404 132 60 22
BanII G_rGCy'C
Cuts at: 0 11 353 618
Size: 11 342 265
Fragments arranged by size:
342 265 11
BbeI G_GCGC'C
Cuts at: 0 64 86 218 618
Size: 64 22 132 400
Fragments arranged by size:
400 132 64 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 562 618
Size: 562 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 516 550 618
Size: 516 34 68
Fragments arranged by size:
516 68 34
BglI GCCn_nnn'nGGC
Cuts at: 0 58 80 311 618
Size: 58 22 231 307
Fragments arranged by size:
307 231 58 22
Bme1580I G_kGCm'C
Cuts at: 0 11 466 618
Size: 11 455 152
Fragments arranged by size:
455 152 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 148 271 367 618
Size: 136 12 123 96 251
Fragments arranged by size:
251 136 123 96 12
Bpu10I CC'TnA_GC
Cuts at: 0 541 618
Size: 541 77
BsaHI Gr'CG_yC
Cuts at: 0 61 83 215 618
Size: 61 22 132 403
Fragments arranged by size:
403 132 61 22
BseYI C'CCAG_C
Cuts at: 0 137 231 618
Size: 137 94 387
Fragments arranged by size:
387 137 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 618
Size: 83 535
BsiEI CG_ry'CG
Cuts at: 0 20 203 306 618
Size: 20 183 103 312
Fragments arranged by size:
312 183 103 20
BsiHKAI G_wGCw'C
Cuts at: 0 353 466 618
Size: 353 113 152
Fragments arranged by size:
353 152 113
Bsp1286I G_dGCh'C
Cuts at: 0 11 353 466 618
Size: 11 342 113 152
Fragments arranged by size:
342 152 113 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 618
Size: 236 382
BsrBI CCG'CTC
Cuts at: 0 212 366 618
Size: 212 154 252
Fragments arranged by size:
252 212 154
BsrFI r'CCGG_y
Cuts at: 0 174 217 398 618
Size: 174 43 181 220
Fragments arranged by size:
220 181 174 43
BssHII G'CGCG_C
Cuts at: 0 166 317 618
Size: 166 151 301
Fragments arranged by size:
301 166 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 97 618
Size: 97 521
BstEII G'GTnAC_C
Cuts at: 0 594 618
Size: 594 24
BtgI C'CryG_G
Cuts at: 0 20 359 618
Size: 20 339 259
Fragments arranged by size:
339 259 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 519 618
Size: 519 99
BtsI GCAGTG_nn'
Cuts at: 0 95 618
Size: 95 523
EaeI y'GGCC_r
Cuts at: 0 17 200 303 392 410 618
Size: 17 183 103 89 18 208
Fragments arranged by size:
208 183 103 89 18 17
EagI C'GGCC_G
Cuts at: 0 17 200 303 618
Size: 17 183 103 315
Fragments arranged by size:
315 183 103 17
EcoICRI GAG'CTC
Cuts at: 0 351 618
Size: 351 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 148 271 367 618
Size: 136 12 123 96 251
Fragments arranged by size:
251 136 123 96 12
EcoO109I rG'GnC_Cy
Cuts at: 0 7 618
Size: 7 611
HaeII r_GCGC'y
Cuts at: 0 31 64 86 135 157 218 295 618
Size: 31 33 22 49 22 61 77 323
Fragments arranged by size:
323 77 61 49 33 31 22 22
KasI G'GCGC_C
Cuts at: 0 60 82 214 618
Size: 60 22 132 404
Fragments arranged by size:
404 132 60 22
MscI TGG'CCA
Cuts at: 0 394 412 618
Size: 394 18 206
Fragments arranged by size:
394 206 18
MspA1I CmG'CkG
Cuts at: 0 22 137 618
Size: 22 115 481
Fragments arranged by size:
481 115 22
NaeI GCC'GGC
Cuts at: 0 219 618
Size: 219 399
NarI GG'CG_CC
Cuts at: 0 61 83 215 618
Size: 61 22 132 403
Fragments arranged by size:
403 132 61 22
NgoMIV G'CCGG_C
Cuts at: 0 217 618
Size: 217 401
NspI r_CATG'y
Cuts at: 0 531 618
Size: 531 87
PasI CC'CwG_GG
Cuts at: 0 65 618
Size: 65 553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 618
Size: 451 32 135
Fragments arranged by size:
451 135 32
PspOMI G'GGCC_C
Cuts at: 0 7 618
Size: 7 611
SacI G_AGCT'C
Cuts at: 0 353 618
Size: 353 265
SacII CC_GC'GG
Cuts at: 0 23 618
Size: 23 595
SfoI GGC'GCC
Cuts at: 0 62 84 216 618
Size: 62 22 132 402
Fragments arranged by size:
402 132 62 22
SgrAI Cr'CCGG_yG
Cuts at: 0 217 618
Size: 217 401
SmaI CCC'GGG
Cuts at: 0 12 618
Size: 12 606
StyI C'CwwG_G
Cuts at: 0 191 618
Size: 191 427
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 117 613 618
Size: 108 9 496 5
Fragments arranged by size:
496 108 9 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 618
Size: 111 507
XmaI C'CCGG_G
Cuts at: 0 10 618
Size: 10 608
Enzymes that do cut:
AfeI ApaI ApaLI AscI AvaI BanI BanII BbeI
BbsI BcgI BglI Bme1580I BpmI Bpu10I BsaHI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstEII BtgI BtgZI BtsI EaeI EagI EcoICRI
Eco57MI EcoO109I HaeII KasI MscI MspA1I NaeI NarI
NgoMIV NspI PasI PpiI PspOMI SacI SacII SfoI
SgrAI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI