Restriction map for
pFN21AE2290
(Linear) (Six-base) MAPSORT of: KIEE2290.seq Check: 6496 from: 1 to: 1074
KIEE2290.orf
With 182 enzymes: SgfI *
July 15, 2010 18:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 630 1074
Size: 339 291 444
Fragments arranged by size:
444 339 291
AfeI AGC'GCT
Cuts at: 0 400 1074
Size: 400 674
AhdI GACnn_n'nnGTC
Cuts at: 0 24 1074
Size: 24 1050
AlwNI CAG_nnn'CTG
Cuts at: 0 294 1074
Size: 294 780
ApaI G_GGCC'C
Cuts at: 0 741 1074
Size: 741 333
ApaLI G'TGCA_C
Cuts at: 0 324 1074
Size: 324 750
AscI GG'CGCG_CC
Cuts at: 0 139 1074
Size: 139 935
AseI AT'TA_AT
Cuts at: 0 917 1074
Size: 917 157
AvaI C'yCGr_G
Cuts at: 0 238 310 547 733 1074
Size: 238 72 237 186 341
Fragments arranged by size:
341 238 237 186 72
BanI G'GyrC_C
Cuts at: 0 306 416 1074
Size: 306 110 658
Fragments arranged by size:
658 306 110
BanII G_rGCy'C
Cuts at: 0 187 741 1074
Size: 187 554 333
Fragments arranged by size:
554 333 187
BbeI G_GCGC'C
Cuts at: 0 310 1074
Size: 310 764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 315 349 357 391 1074
Size: 315 34 8 34 683
Fragments arranged by size:
683 315 34 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 728 1074
Size: 728 346
Bme1580I G_kGCm'C
Cuts at: 0 328 419 741 1074
Size: 328 91 322 333
Fragments arranged by size:
333 328 322 91
BmgBI CAC'GTC
Cuts at: 0 159 1074
Size: 159 915
BmrI ACTGGGnnnn_n'
Cuts at: 0 18 253 1074
Size: 18 235 821
Fragments arranged by size:
821 235 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1074
Size: 415 659
BsaAI yAC'GTr
Cuts at: 0 204 1074
Size: 204 870
BsaHI Gr'CG_yC
Cuts at: 0 307 1074
Size: 307 767
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 1074
Size: 787 287
BseYI C'CCAG_C
Cuts at: 0 72 108 1074
Size: 72 36 966
Fragments arranged by size:
966 72 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 1074
Size: 186 888
BsiEI CG_ry'CG
Cuts at: 0 101 177 413 1074
Size: 101 76 236 661
Fragments arranged by size:
661 236 101 76
BsiHKAI G_wGCw'C
Cuts at: 0 187 328 635 1074
Size: 187 141 307 439
Fragments arranged by size:
439 307 187 141
BsiWI C'GTAC_G
Cuts at: 0 275 1074
Size: 275 799
BsmBI CGTCTCn'nnnn_
Cuts at: 0 34 447 769 1074
Size: 34 413 322 305
Fragments arranged by size:
413 322 305 34
Bsp1286I G_dGCh'C
Cuts at: 0 187 328 419 635 741 1074
Size: 187 141 91 216 106 333
Fragments arranged by size:
333 216 187 141 106 91
BspHI T'CATG_A
Cuts at: 0 803 1074
Size: 803 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 253 596 1074
Size: 242 11 343 478
Fragments arranged by size:
478 343 242 11
BsrDI GCAATG_nn'
Cuts at: 0 12 1074
Size: 12 1062
BsrFI r'CCGG_y
Cuts at: 0 172 1074
Size: 172 902
BssHII G'CGCG_C
Cuts at: 0 139 1074
Size: 139 935
BstEII G'GTnAC_C
Cuts at: 0 210 1074
Size: 210 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 1074
Size: 393 681
Bsu36I CC'TnA_GG
Cuts at: 0 952 1074
Size: 952 122
BtgI C'CryG_G
Cuts at: 0 386 764 1074
Size: 386 378 310
Fragments arranged by size:
386 378 310
BtsI GCAGTG_nn'
Cuts at: 0 723 1074
Size: 723 351
DrdI GACnn_nn'nnGTC
Cuts at: 0 937 1074
Size: 937 137
EaeI y'GGCC_r
Cuts at: 0 98 174 362 461 720 1074
Size: 98 76 188 99 259 354
Fragments arranged by size:
354 259 188 99 98 76
EagI C'GGCC_G
Cuts at: 0 98 174 1074
Size: 98 76 900
Fragments arranged by size:
900 98 76
EarI CTCTTCn'nnn_
Cuts at: 0 385 502 873 1074
Size: 385 117 371 201
Fragments arranged by size:
385 371 201 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 591 784 1074
Size: 591 193 290
Fragments arranged by size:
591 290 193
EcoICRI GAG'CTC
Cuts at: 0 185 1074
Size: 185 889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 415 630 1074
Size: 339 76 215 444
Fragments arranged by size:
444 339 215 76
EcoO109I rG'GnC_Cy
Cuts at: 0 242 456 551 737 888 1074
Size: 242 214 95 186 151 186
Fragments arranged by size:
242 214 186 186 151 95
HaeII r_GCGC'y
Cuts at: 0 133 173 310 402 438 1074
Size: 133 40 137 92 36 636
Fragments arranged by size:
636 137 133 92 40 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1074
Size: 10 32 1032
Fragments arranged by size:
1032 32 10
KasI G'GCGC_C
Cuts at: 0 306 1074
Size: 306 768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1074
Size: 976 98
MscI TGG'CCA
Cuts at: 0 364 1074
Size: 364 710
MslI CAynn'nnrTG
Cuts at: 0 670 1074
Size: 670 404
MspA1I CmG'CkG
Cuts at: 0 146 513 594 698 1074
Size: 146 367 81 104 376
Fragments arranged by size:
376 367 146 104 81
NaeI GCC'GGC
Cuts at: 0 174 1074
Size: 174 900
NarI GG'CG_CC
Cuts at: 0 307 1074
Size: 307 767
NcoI C'CATG_G
Cuts at: 0 386 764 1074
Size: 386 378 310
Fragments arranged by size:
386 378 310
NgoMIV G'CCGG_C
Cuts at: 0 172 1074
Size: 172 902
NspI r_CATG'y
Cuts at: 0 675 1074
Size: 675 399
PasI CC'CwG_GG
Cuts at: 0 711 1074
Size: 711 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 294 1074
Size: 207 87 780
Fragments arranged by size:
780 207 87
PmlI CAC'GTG
Cuts at: 0 204 1074
Size: 204 870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 1074
Size: 208 32 834
Fragments arranged by size:
834 208 32
PpuMI rG'GwC_Cy
Cuts at: 0 242 551 888 1074
Size: 242 309 337 186
Fragments arranged by size:
337 309 242 186
PspOMI G'GGCC_C
Cuts at: 0 737 1074
Size: 737 337
PstI C_TGCA'G
Cuts at: 0 593 1074
Size: 593 481
PvuI CG_AT'CG
Cuts at: 0 413 1074
Size: 413 661
PvuII CAG'CTG
Cuts at: 0 594 1074
Size: 594 480
RsrII CG'GwC_CG
Cuts at: 0 578 1074
Size: 578 496
SacI G_AGCT'C
Cuts at: 0 187 1074
Size: 187 887
SapI GCTCTTCn'nnn_
Cuts at: 0 385 502 1074
Size: 385 117 572
Fragments arranged by size:
572 385 117
ScaI AGT'ACT
Cuts at: 0 541 1074
Size: 541 533
SexAI A'CCwGG_T
Cuts at: 0 553 1074
Size: 553 521
SfcI C'TryA_G
Cuts at: 0 589 1074
Size: 589 485
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 728 1074
Size: 728 346
SfoI GGC'GCC
Cuts at: 0 308 1074
Size: 308 766
SmaI CCC'GGG
Cuts at: 0 549 735 1074
Size: 549 186 339
Fragments arranged by size:
549 339 186
SphI G_CATG'C
Cuts at: 0 675 1074
Size: 675 399
StuI AGG'CCT
Cuts at: 0 957 1074
Size: 957 117
StyI C'CwwG_G
Cuts at: 0 386 764 934 1074
Size: 386 378 170 140
Fragments arranged by size:
386 378 170 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 229 1074
Size: 229 845
TatI w'GTAC_w
Cuts at: 0 539 1074
Size: 539 535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 1074
Size: 148 32 894
Fragments arranged by size:
894 148 32
XmaI C'CCGG_G
Cuts at: 0 547 733 1074
Size: 547 186 341
Fragments arranged by size:
547 341 186
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaI ApaLI AscI AseI
AvaI BanI BanII BbeI BcgI BglI Bme1580I BmgBI
BmrI BpmI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BssHII BstEII BstXI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SapI ScaI SexAI
SfcI SfiI SfoI SmaI SphI StuI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApoI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsmI
BspEI BsrBI BsrGI BssSI BstAPI BstBI BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PshAI PsiI PspXI PsrI SacII
SalI SanDI SbfI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI