Restriction map for
pFN21AE2289
(Linear) (Six-base) MAPSORT of: KIEE2289.seq Check: 3901 from: 1 to: 1524
KIEE2289.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AccI GT'mk_AC
Cuts at: 0 698 1022 1319 1340 1524
Size: 698 324 297 21 184
Fragments arranged by size:
698 324 297 184 21
Acc65I G'GTAC_C
Cuts at: 0 107 1524
Size: 107 1417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1215 1524
Size: 1215 309
AhdI GACnn_n'nnGTC
Cuts at: 0 93 1524
Size: 93 1431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 1524
Size: 464 1060
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 1524
Size: 334 32 1158
Fragments arranged by size:
1158 334 32
AlwNI CAG_nnn'CTG
Cuts at: 0 15 1524
Size: 15 1509
ApaI G_GGCC'C
Cuts at: 0 234 899 964 1524
Size: 234 665 65 560
Fragments arranged by size:
665 560 234 65
ApaLI G'TGCA_C
Cuts at: 0 562 1524
Size: 562 962
AseI AT'TA_AT
Cuts at: 0 1082 1524
Size: 1082 442
AvaI C'yCGr_G
Cuts at: 0 374 1524
Size: 374 1150
BamHI G'GATC_C
Cuts at: 0 264 749 1524
Size: 264 485 775
Fragments arranged by size:
775 485 264
BanI G'GyrC_C
Cuts at: 0 78 107 1346 1524
Size: 78 29 1239 178
Fragments arranged by size:
1239 178 78 29
BanII G_rGCy'C
Cuts at: 0 234 899 964 998 1192 1524
Size: 234 665 65 34 194 332
Fragments arranged by size:
665 332 234 194 65 34
BbeI G_GCGC'C
Cuts at: 0 82 1350 1524
Size: 82 1268 174
Fragments arranged by size:
1268 174 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 581 913 1524
Size: 286 295 332 611
Fragments arranged by size:
611 332 295 286
BciVI GTATCCnnnnn_n'
Cuts at: 0 1479 1524
Size: 1479 45
BglI GCCn_nnn'nGGC
Cuts at: 0 229 688 1524
Size: 229 459 836
Fragments arranged by size:
836 459 229
Bme1580I G_kGCm'C
Cuts at: 0 234 566 665 827 836 899 964 1524
Size: 234 332 99 162 9 63 65 560
Fragments arranged by size:
560 332 234 162 99 65 63 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 688 823 839 1524
Size: 688 135 16 685
Fragments arranged by size:
688 685 135 16
BmtI G_CTAG'C
Cuts at: 0 1219 1524
Size: 1219 305
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 263 1524
Size: 263 1261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 426 1524
Size: 330 96 1098
Fragments arranged by size:
1098 330 96
BsaHI Gr'CG_yC
Cuts at: 0 79 1347 1524
Size: 79 1268 177
Fragments arranged by size:
1268 177 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 1052 1082 1524
Size: 334 30 688 30 442
Fragments arranged by size:
688 442 334 30 30
BseYI C'CCAG_C
Cuts at: 0 21 300 552 891 1524
Size: 21 279 252 339 633
Fragments arranged by size:
633 339 279 252 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 712 1524
Size: 460 252 812
Fragments arranged by size:
812 460 252
BsiHKAI G_wGCw'C
Cuts at: 0 566 1524
Size: 566 958
Bsp1286I G_dGCh'C
Cuts at: 0 234 566 665 827 836 899 964 998
Size: 234 332 99 162 9 63 65 34
Cuts at: 998 1192 1524
Size: 194 332
Fragments arranged by size:
332 332 234 194 162 99 65 63
34 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 623 1524
Size: 353 270 901
Fragments arranged by size:
901 353 270
BsrDI GCAATG_nn'
Cuts at: 0 417 623 1524
Size: 417 206 901
Fragments arranged by size:
901 417 206
BsrFI r'CCGG_y
Cuts at: 0 710 1524
Size: 710 814
BstXI CCAn_nnnn'nTGG
Cuts at: 0 891 1524
Size: 891 633
BstYI r'GATC_y
Cuts at: 0 130 264 749 1524
Size: 130 134 485 775
Fragments arranged by size:
775 485 134 130
Bsu36I CC'TnA_GG
Cuts at: 0 1464 1524
Size: 1464 60
BtgI C'CryG_G
Cuts at: 0 83 474 603 900 1524
Size: 83 391 129 297 624
Fragments arranged by size:
624 391 297 129 83
BtsI GCAGTG_nn'
Cuts at: 0 564 1210 1524
Size: 564 646 314
Fragments arranged by size:
646 564 314
EaeI y'GGCC_r
Cuts at: 0 477 1311 1524
Size: 477 834 213
Fragments arranged by size:
834 477 213
EarI CTCTTCn'nnn_
Cuts at: 0 766 797 1524
Size: 766 31 727
Fragments arranged by size:
766 727 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 1524
Size: 451 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 426 1215 1524
Size: 330 96 789 309
Fragments arranged by size:
789 330 309 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 988 1183 1524
Size: 125 863 195 341
Fragments arranged by size:
863 341 195 125
EcoO109I rG'GnC_Cy
Cuts at: 0 57 230 596 681 895 896 961 1524
Size: 57 173 366 85 214 1 65 563
Fragments arranged by size:
563 366 214 173 85 65 57 1
EcoRV GAT'ATC
Cuts at: 0 156 1524
Size: 156 1368
FspI TGC'GCA
Cuts at: 0 619 1524
Size: 619 905
HaeII r_GCGC'y
Cuts at: 0 82 1077 1350 1524
Size: 82 995 273 174
Fragments arranged by size:
995 273 174 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 741 773 1398 1430 1524
Size: 79 32 630 32 625 32 94
Fragments arranged by size:
630 625 94 79 32 32 32
KasI G'GCGC_C
Cuts at: 0 78 1346 1524
Size: 78 1268 178
Fragments arranged by size:
1268 178 78
KpnI G_GTAC'C
Cuts at: 0 111 1524
Size: 111 1413
MslI CAynn'nnrTG
Cuts at: 0 734 1524
Size: 734 790
MspA1I CmG'CkG
Cuts at: 0 15 213 308 1524
Size: 15 198 95 1216
Fragments arranged by size:
1216 198 95 15
NarI GG'CG_CC
Cuts at: 0 79 1347 1524
Size: 79 1268 177
Fragments arranged by size:
1268 177 79
NcoI C'CATG_G
Cuts at: 0 474 1524
Size: 474 1050
NheI G'CTAG_C
Cuts at: 0 1215 1524
Size: 1215 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 651 1447 1524
Size: 639 12 796 77
Fragments arranged by size:
796 639 77 12
PfoI T'CCnGG_A
Cuts at: 0 398 1524
Size: 398 1126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1524
Size: 334 32 1158
Fragments arranged by size:
1158 334 32
PpuMI rG'GwC_Cy
Cuts at: 0 57 596 1524
Size: 57 539 928
Fragments arranged by size:
928 539 57
PspOMI G'GGCC_C
Cuts at: 0 230 895 960 1524
Size: 230 665 65 564
Fragments arranged by size:
665 564 230 65
PvuII CAG'CTG
Cuts at: 0 15 213 1524
Size: 15 198 1311
Fragments arranged by size:
1311 198 15
SfcI C'TryA_G
Cuts at: 0 112 1427 1524
Size: 112 1315 97
Fragments arranged by size:
1315 112 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 688 1524
Size: 688 836
SfoI GGC'GCC
Cuts at: 0 80 1348 1524
Size: 80 1268 176
Fragments arranged by size:
1268 176 80
SmaI CCC'GGG
Cuts at: 0 376 1524
Size: 376 1148
SrfI GCCC'GGGC
Cuts at: 0 376 1524
Size: 376 1148
SspI AAT'ATT
Cuts at: 0 1392 1524
Size: 1392 132
StyI C'CwwG_G
Cuts at: 0 474 1481 1524
Size: 474 1007 43
Fragments arranged by size:
1007 474 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 746 1524
Size: 746 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 919 951 1052 1084 1524
Size: 588 32 299 32 101 32 440
Fragments arranged by size:
588 440 299 101 32 32 32
XbaI T'CTAG_A
Cuts at: 0 123 1524
Size: 123 1401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 760 1524
Size: 760 764
XmaI C'CCGG_G
Cuts at: 0 374 1524
Size: 374 1150
XmnI GAAnn'nnTTC
Cuts at: 0 1392 1524
Size: 1392 132
Enzymes that do cut:
AccI Acc65I AcuI AhdI AlfI AloI AlwNI ApaI
ApaLI AseI AvaI BamHI BanI BanII BbeI BbsI
BciVI BglI Bme1580I BmrI BmtI BplI BpmI BsaHI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI
KpnI MslI MspA1I NarI NcoI NheI PflMI PfoI
PpiI PpuMI PspOMI PvuII SfcI SfiI SfoI SmaI
SrfI SspI StyI TaqII TstI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AleI
ApoI AscI AsiSI AvrII BaeI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI FalI FseI FspAI HincII HindIII HpaI
MfeI MluI MmeI MscI NaeI NdeI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SmlI SnaBI SpeI SphI StuI SwaI TatI
Tth111I XhoI ZraI