(Linear) (Six-base) MAPSORT of: KIEE2288.seq  Check: 8705  from: 1  to: 861



KIEE2288.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     861 
   Size:        244     617
 
AleI CACnn'nnGTG

Cuts at:      0     509     861 
   Size:        509     352
 
BanII G_rGCy'C

Cuts at:      0     601     861 
   Size:        601     260
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     225     861 
   Size:        225     636
 
BclI T'GATC_A

Cuts at:      0     182     861 
   Size:        182     679
 
BlpI GC'TnA_GC

Cuts at:      0     441     861 
   Size:        441     420
 
Bme1580I G_kGCm'C

Cuts at:      0     317     861 
   Size:        317     544
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     614     861 
   Size:        614     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     822     861 
   Size:        364     458      39

  Fragments arranged by size: 

                 458     364      39
 
BsaAI yAC'GTr

Cuts at:      0     200     434     861 
   Size:        200     234     427

  Fragments arranged by size: 

                 427     234     200
 
BsaWI w'CCGG_w

Cuts at:      0     326     861 
   Size:        326     535
 
BseYI C'CCAG_C

Cuts at:      0     173     861 
   Size:        173     688
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     861 
   Size:        456     405
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     861 
   Size:         38     823
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     317     601     861 
   Size:         38     279     284     260

  Fragments arranged by size: 

                 284     279     260      38
 
BssSI C'ACGA_G

Cuts at:      0     712     861 
   Size:        712     149
 
BstYI r'GATC_y

Cuts at:      0     127     566     861 
   Size:        127     439     295

  Fragments arranged by size: 

                 439     295     127
 
BtgI C'CryG_G

Cuts at:      0     695     861 
   Size:        695     166
 
DraIII CAC_nnn'GTG

Cuts at:      0     850     861 
   Size:        850      11
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     556     861 
   Size:        556     305
 
EaeI y'GGCC_r

Cuts at:      0     473     524     692     725     861 
   Size:        473      51     168      33     136

  Fragments arranged by size: 

                 473     168     136      51      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     364     822     861 
   Size:        244     120     458      39

  Fragments arranged by size: 

                 458     244     120      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     541     861 
   Size:        541     320
 
HaeII r_GCGC'y

Cuts at:      0     291     861 
   Size:        291     570
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     695     727     861 
   Size:        695      32     134

  Fragments arranged by size: 

                 695     134      32
 
HindIII A'AGCT_T

Cuts at:      0     161     861 
   Size:        161     700
 
MscI TGG'CCA

Cuts at:      0     475     727     861 
   Size:        475     252     134

  Fragments arranged by size: 

                 475     252     134
 
MslI CAynn'nnrTG

Cuts at:      0     509     694     861 
   Size:        509     185     167

  Fragments arranged by size: 

                 509     185     167
 
MspA1I CmG'CkG

Cuts at:      0     177     861 
   Size:        177     684
 
PmlI CAC'GTG

Cuts at:      0     434     861 
   Size:        434     427
 
PpuMI rG'GwC_Cy

Cuts at:      0     541     861 
   Size:        541     320
 
PstI C_TGCA'G

Cuts at:      0     390     861 
   Size:        390     471
 
PvuII CAG'CTG

Cuts at:      0     177     861 
   Size:        177     684
 
SfcI C'TryA_G

Cuts at:      0     318     386     570     861 
   Size:        318      68     184     291

  Fragments arranged by size: 

                 318     291     184      68
 
SspI AAT'ATT

Cuts at:      0     423     861 
   Size:        423     438
 
StyI C'CwwG_G

Cuts at:      0     119     476     629     861 
   Size:        119     357     153     232

  Fragments arranged by size: 

                 357     232     153     119
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     534     627     861 
   Size:        534      93     234

  Fragments arranged by size: 

                 534     234      93
 

 Enzymes that do cut:

     AcuI     AleI    BanII     BbsI     BclI     BlpI Bme1580I     BmrI
     BpmI    BsaAI    BsaWI    BseYI     BsgI  BsiHKAI Bsp1286I    BssSI
    BstYI     BtgI   DraIII     DrdI     EaeI  Eco57MI EcoO109I    HaeII
    Hin4I  HindIII     MscI     MslI   MspA1I     PmlI    PpuMI     PstI
    PvuII     SfcI     SspI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI