(Linear) (Six-base) MAPSORT of: KIEE2285.seq  Check: 8174  from: 1  to: 498



KIEE2285.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AccI GT'mk_AC

Cuts at:      0     191     498 
   Size:        191     307
 
AflII C'TTAA_G

Cuts at:      0     331     498 
   Size:        331     167
 
ApaI G_GGCC'C

Cuts at:      0     315     498 
   Size:        315     183
 
AvaI C'yCGr_G

Cuts at:      0     316     498 
   Size:        316     182
 
BanII G_rGCy'C

Cuts at:      0     315     498 
   Size:        315     183
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      59     498 
   Size:         59     439
 
Bme1580I G_kGCm'C

Cuts at:      0     315     498 
   Size:        315     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     498 
   Size:        166     332
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     262     498 
   Size:        262     236
 
BsaHI Gr'CG_yC

Cuts at:      0     365     498 
   Size:        365     133
 
BsaWI w'CCGG_w

Cuts at:      0       7     498 
   Size:          7     491
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     163     436     498 
   Size:         94      69     273      62

  Fragments arranged by size: 

                 273      94      69      62
 
BseYI C'CCAG_C

Cuts at:      0     456     498 
   Size:        456      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     498 
   Size:        423      75
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     498 
   Size:        110     388
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     315     498 
   Size:        110     205     183

  Fragments arranged by size: 

                 205     183     110
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     402     498 
   Size:        402      96
 
BstYI r'GATC_y

Cuts at:      0     161     291     498 
   Size:        161     130     207

  Fragments arranged by size: 

                 207     161     130
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     188     498 
   Size:        188     310
 
BtsI GCAGTG_nn'

Cuts at:      0     471     498 
   Size:        471      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     115     498 
   Size:        115     383
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     498 
   Size:        166     332
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     121     148     498 
   Size:        121      27     350

  Fragments arranged by size: 

                 350     121      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     312     498 
   Size:        312     186
 
HincII GTy'rAC

Cuts at:      0     192     498 
   Size:        192     306
 
MslI CAynn'nnrTG

Cuts at:      0      37      55     498 
   Size:         37      18     443

  Fragments arranged by size: 

                 443      37      18
 
MspA1I CmG'CkG

Cuts at:      0     437     498 
   Size:        437      61
 
PfoI T'CCnGG_A

Cuts at:      0     143     498 
   Size:        143     355
 
PspOMI G'GGCC_C

Cuts at:      0     311     498 
   Size:        311     187
 
SalI G'TCGA_C

Cuts at:      0     190     498 
   Size:        190     308
 
SapI GCTCTTCn'nnn_

Cuts at:      0     115     498 
   Size:        115     383
 
SmlI C'TyrA_G

Cuts at:      0     331     498 
   Size:        331     167
 
StyI C'CwwG_G

Cuts at:      0     272     359     498 
   Size:        272      87     139

  Fragments arranged by size: 

                 272     139      87
 

 Enzymes that do cut:

     AccI    AflII     ApaI     AvaI    BanII     BbsI Bme1580I     BpmI
     BsaI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
   BstAPI    BstYI    BtgZI     BtsI     EarI  Eco57MI    EcoNI EcoO109I
   HincII     MslI   MspA1I     PfoI   PspOMI     SalI     SapI     SmlI
     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI