Restriction map for
pFN21AE2278
(Linear) (Six-base) MAPSORT of: KIEE2278.seq Check: 9719 from: 1 to: 1026
KIEE2278.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 450 1026
Size: 450 576
AccI GT'mk_AC
Cuts at: 0 56 1026
Size: 56 970
Acc65I G'GTAC_C
Cuts at: 0 409 1026
Size: 409 617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1026
Size: 956 70
AfeI AGC'GCT
Cuts at: 0 91 1026
Size: 91 935
AflIII A'CryG_T
Cuts at: 0 569 1026
Size: 569 457
ApaI G_GGCC'C
Cuts at: 0 825 1026
Size: 825 201
AvaI C'yCGr_G
Cuts at: 0 899 1026
Size: 899 127
BamHI G'GATC_C
Cuts at: 0 227 393 1026
Size: 227 166 633
Fragments arranged by size:
633 227 166
BanI G'GyrC_C
Cuts at: 0 409 1026
Size: 409 617
BanII G_rGCy'C
Cuts at: 0 825 1026
Size: 825 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 505 656 1026
Size: 505 151 370
Fragments arranged by size:
505 370 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 756 790 1026
Size: 756 34 236
Fragments arranged by size:
756 236 34
BglI GCCn_nnn'nGGC
Cuts at: 0 718 1026
Size: 718 308
BglII A'GATC_T
Cuts at: 0 26 1026
Size: 26 1000
Bme1580I G_kGCm'C
Cuts at: 0 825 1026
Size: 825 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 496 1026
Size: 496 530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 340 469 1026
Size: 90 250 129 557
Fragments arranged by size:
557 250 129 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1026
Size: 399 627
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 1026
Size: 304 722
BsaAI yAC'GTr
Cuts at: 0 495 1026
Size: 495 531
BsaBI GATnn'nnATC
Cuts at: 0 398 988 1026
Size: 398 590 38
Fragments arranged by size:
590 398 38
BsaHI Gr'CG_yC
Cuts at: 0 245 1026
Size: 245 781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 285 315 1026
Size: 285 30 711
Fragments arranged by size:
711 285 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 175 1026
Size: 136 39 851
Fragments arranged by size:
851 136 39
BseYI C'CCAG_C
Cuts at: 0 105 503 1026
Size: 105 398 523
Fragments arranged by size:
523 398 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 831 976 1026
Size: 750 81 145 50
Fragments arranged by size:
750 145 81 50
BsiHKAI G_wGCw'C
Cuts at: 0 363 1026
Size: 363 663
Bsp1286I G_dGCh'C
Cuts at: 0 363 825 1026
Size: 363 462 201
Fragments arranged by size:
462 363 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 450 1026
Size: 450 576
BsrDI GCAATG_nn'
Cuts at: 0 812 1026
Size: 812 214
BssSI C'ACGA_G
Cuts at: 0 481 1026
Size: 481 545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 1026
Size: 723 303
BstYI r'GATC_y
Cuts at: 0 26 227 393 1026
Size: 26 201 166 633
Fragments arranged by size:
633 201 166 26
BtgI C'CryG_G
Cuts at: 0 716 722 1026
Size: 716 6 304
Fragments arranged by size:
716 304 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 76 1026
Size: 76 950
BtsI GCAGTG_nn'
Cuts at: 0 667 848 964 1026
Size: 667 181 116 62
Fragments arranged by size:
667 181 116 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 1026
Size: 526 500
EaeI y'GGCC_r
Cuts at: 0 489 710 719 1026
Size: 489 221 9 307
Fragments arranged by size:
489 307 221 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 607 1026
Size: 607 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 340 469 956 1026
Size: 90 250 129 487 70
Fragments arranged by size:
487 250 129 90 70
EcoO109I rG'GnC_Cy
Cuts at: 0 696 822 912 1026
Size: 696 126 90 114
Fragments arranged by size:
696 126 114 90
HaeII r_GCGC'y
Cuts at: 0 46 93 106 1026
Size: 46 47 13 920
Fragments arranged by size:
920 47 46 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 788 820 1026
Size: 788 32 206
Fragments arranged by size:
788 206 32
KpnI G_GTAC'C
Cuts at: 0 413 1026
Size: 413 613
MscI TGG'CCA
Cuts at: 0 491 712 721 1026
Size: 491 221 9 305
Fragments arranged by size:
491 305 221 9
MslI CAynn'nnrTG
Cuts at: 0 721 1026
Size: 721 305
MspA1I CmG'CkG
Cuts at: 0 507 856 934 1026
Size: 507 349 78 92
Fragments arranged by size:
507 349 92 78
NcoI C'CATG_G
Cuts at: 0 716 722 1026
Size: 716 6 304
Fragments arranged by size:
716 304 6
NspI r_CATG'y
Cuts at: 0 434 573 803 1026
Size: 434 139 230 223
Fragments arranged by size:
434 230 223 139
PciI A'CATG_T
Cuts at: 0 569 1026
Size: 569 457
PmlI CAC'GTG
Cuts at: 0 495 1026
Size: 495 531
PpuMI rG'GwC_Cy
Cuts at: 0 696 912 1026
Size: 696 216 114
Fragments arranged by size:
696 216 114
PspOMI G'GGCC_C
Cuts at: 0 821 1026
Size: 821 205
PstI C_TGCA'G
Cuts at: 0 855 1026
Size: 855 171
PvuII CAG'CTG
Cuts at: 0 507 934 1026
Size: 507 427 92
Fragments arranged by size:
507 427 92
SfcI C'TryA_G
Cuts at: 0 851 1026
Size: 851 175
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 718 1026
Size: 718 308
SmlI C'TyrA_G
Cuts at: 0 414 1026
Size: 414 612
StyI C'CwwG_G
Cuts at: 0 716 722 1026
Size: 716 6 304
Fragments arranged by size:
716 304 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 215 574 680 1026
Size: 215 359 106 346
Fragments arranged by size:
359 346 215 106
TatI w'GTAC_w
Cuts at: 0 125 1026
Size: 125 901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 285 317 722 754 1026
Size: 285 32 405 32 272
Fragments arranged by size:
405 285 272 32 32
XbaI T'CTAG_A
Cuts at: 0 624 747 1026
Size: 624 123 279
Fragments arranged by size:
624 279 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 720 729 1026
Size: 444 276 9 297
Fragments arranged by size:
444 297 276 9
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII ApaI AvaI
BamHI BanI BanII BbsI BcgI BglI BglII Bme1580I
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EciI
Eco57MI EcoO109I HaeII Hin4I KpnI MscI MslI MspA1I
NcoI NspI PciI PmlI PpuMI PspOMI PstI PvuII
SfcI SfiI SmlI StyI TaqII TatI TstI XbaI
XcmI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI Bpu10I BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI XmnI ZraI