Restriction map for
pFN21AE2274
(Linear) (Six-base) MAPSORT of: KIEE2274.seq Check: 8489 from: 1 to: 897
KIEE2274.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 405 897
Size: 405 492
ApaI G_GGCC'C
Cuts at: 0 79 897
Size: 79 818
AvaI C'yCGr_G
Cuts at: 0 851 897
Size: 851 46
BamHI G'GATC_C
Cuts at: 0 88 897
Size: 88 809
BanI G'GyrC_C
Cuts at: 0 295 478 897
Size: 295 183 419
Fragments arranged by size:
419 295 183
BanII G_rGCy'C
Cuts at: 0 18 79 897
Size: 18 61 818
Fragments arranged by size:
818 61 18
BbvCI CC'TCA_GC
Cuts at: 0 250 897
Size: 250 647
BciVI GTATCCnnnnn_n'
Cuts at: 0 309 897
Size: 309 588
Bme1580I G_kGCm'C
Cuts at: 0 79 897
Size: 79 818
BmtI G_CTAG'C
Cuts at: 0 598 897
Size: 598 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 621 721 897
Size: 289 332 100 176
Fragments arranged by size:
332 289 176 100
Bpu10I CC'TnA_GC
Cuts at: 0 250 897
Size: 250 647
BsaI GGTCTCn'nnnn_
Cuts at: 0 181 897
Size: 181 716
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 311 341 897
Size: 311 30 556
Fragments arranged by size:
556 311 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 529 535 871 897
Size: 298 231 6 336 26
Fragments arranged by size:
336 298 231 26 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 168 670 897
Size: 121 47 502 227
Fragments arranged by size:
502 227 121 47
BsiEI CG_ry'CG
Cuts at: 0 219 897
Size: 219 678
Bsp1286I G_dGCh'C
Cuts at: 0 18 79 897
Size: 18 61 818
Fragments arranged by size:
818 61 18
BspHI T'CATG_A
Cuts at: 0 339 897
Size: 339 558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 897
Size: 405 492
BstAPI GCAn_nnn'nTGC
Cuts at: 0 478 897
Size: 478 419
BstEII G'GTnAC_C
Cuts at: 0 631 897
Size: 631 266
BstYI r'GATC_y
Cuts at: 0 88 897
Size: 88 809
BtgI C'CryG_G
Cuts at: 0 68 80 322 897
Size: 68 12 242 575
Fragments arranged by size:
575 242 68 12
BtsI GCAGTG_nn'
Cuts at: 0 109 407 479 897
Size: 109 298 72 418
Fragments arranged by size:
418 298 109 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 731 766 897
Size: 731 35 131
Fragments arranged by size:
731 131 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 195 897
Size: 195 702
EaeI y'GGCC_r
Cuts at: 0 216 413 897
Size: 216 197 484
Fragments arranged by size:
484 216 197
EagI C'GGCC_G
Cuts at: 0 216 897
Size: 216 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 621 721 897
Size: 289 332 100 176
Fragments arranged by size:
332 289 176 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 780 897
Size: 780 117
EcoO109I rG'GnC_Cy
Cuts at: 0 33 76 128 584 897
Size: 33 43 52 456 313
Fragments arranged by size:
456 313 52 43 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 702 734 825 857 897
Size: 212 32 458 32 91 32 40
Fragments arranged by size:
458 212 91 40 32 32 32
MspA1I CmG'CkG
Cuts at: 0 540 897
Size: 540 357
NheI G'CTAG_C
Cuts at: 0 594 897
Size: 594 303
PasI CC'CwG_GG
Cuts at: 0 782 897
Size: 782 115
PfoI T'CCnGG_A
Cuts at: 0 500 897
Size: 500 397
PpuMI rG'GwC_Cy
Cuts at: 0 33 128 897
Size: 33 95 769
Fragments arranged by size:
769 95 33
PspOMI G'GGCC_C
Cuts at: 0 75 897
Size: 75 822
PstI C_TGCA'G
Cuts at: 0 725 897
Size: 725 172
SanDI GG'GwC_CC
Cuts at: 0 33 897
Size: 33 864
SfcI C'TryA_G
Cuts at: 0 721 897
Size: 721 176
StuI AGG'CCT
Cuts at: 0 849 897
Size: 849 48
StyI C'CwwG_G
Cuts at: 0 10 742 897
Size: 10 732 155
Fragments arranged by size:
732 155 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 499 897
Size: 466 33 398
Fragments arranged by size:
466 398 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 307 311 339 343 897
Size: 149 32 126 4 28 4 554
Fragments arranged by size:
554 149 126 32 28 4 4
XmnI GAAnn'nnTTC
Cuts at: 0 791 897
Size: 791 106
Enzymes that do cut:
AarI ApaI AvaI BamHI BanI BanII BbvCI BciVI
Bme1580I BmtI BpmI Bpu10I BsaI BsaXI BseRI BsgI
BsiEI Bsp1286I BspHI BspMI BstAPI BstEII BstYI BtgI
BtsI CspCI DrdI EaeI EagI Eco57MI EcoNI EcoO109I
Hin4I MspA1I NheI PasI PfoI PpuMI PspOMI PstI
SanDI SfcI StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI