Restriction map for
pFN21AE2273
(Linear) (Six-base) MAPSORT of: KIEE2273.seq Check: 8656 from: 1 to: 1677
KIEE2273.orf
With 182 enzymes: SgfI *
March 22, 2012 16:01 ..
AccI GT'mk_AC
Cuts at: 0 590 597 714 887 1677
Size: 590 7 117 173 790
Fragments arranged by size:
790 590 173 117 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1677
Size: 674 1003
AflIII A'CryG_T
Cuts at: 0 1019 1677
Size: 1019 658
AlwNI CAG_nnn'CTG
Cuts at: 0 128 1677
Size: 128 1549
ApoI r'AATT_y
Cuts at: 0 169 236 423 1142 1577 1677
Size: 169 67 187 719 435 100
Fragments arranged by size:
719 435 187 169 100 67
BamHI G'GATC_C
Cuts at: 0 83 1677
Size: 83 1594
BanI G'GyrC_C
Cuts at: 0 1072 1677
Size: 1072 605
BanII G_rGCy'C
Cuts at: 0 44 1677
Size: 44 1633
BbsI GAAGACnn'nnnn_
Cuts at: 0 373 1217 1405 1677
Size: 373 844 188 272
Fragments arranged by size:
844 373 272 188
BglI GCCn_nnn'nGGC
Cuts at: 0 1081 1315 1677
Size: 1081 234 362
Fragments arranged by size:
1081 362 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 239 1406 1677
Size: 239 1167 271
Fragments arranged by size:
1167 271 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 743 1469 1677
Size: 743 726 208
Fragments arranged by size:
743 726 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 1494 1616 1677
Size: 792 702 122 61
Fragments arranged by size:
792 702 122 61
Bpu10I CC'TnA_GC
Cuts at: 0 1604 1677
Size: 1604 73
BsaAI yAC'GTr
Cuts at: 0 1020 1677
Size: 1020 657
BsaHI Gr'CG_yC
Cuts at: 0 97 1591 1677
Size: 97 1494 86
Fragments arranged by size:
1494 97 86
BsaWI w'CCGG_w
Cuts at: 0 674 882 1677
Size: 674 208 795
Fragments arranged by size:
795 674 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 1333 1363 1509 1539 1677
Size: 31 30 1272 30 146 30 138
Fragments arranged by size:
1272 146 138 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 1408 1677
Size: 266 1142 269
Fragments arranged by size:
1142 269 266
BseYI C'CCAG_C
Cuts at: 0 76 335 407 995 1279 1320 1677
Size: 76 259 72 588 284 41 357
Fragments arranged by size:
588 357 284 259 76 72 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1582 1592 1677
Size: 1582 10 85
Fragments arranged by size:
1582 85 10
Bsp1286I G_dGCh'C
Cuts at: 0 44 1677
Size: 44 1633
BsrFI r'CCGG_y
Cuts at: 0 103 1677
Size: 103 1574
BsrGI T'GTAC_A
Cuts at: 0 47 1677
Size: 47 1630
BstBI TT'CG_AA
Cuts at: 0 628 1677
Size: 628 1049
BstYI r'GATC_y
Cuts at: 0 83 125 736 1677
Size: 83 42 611 941
Fragments arranged by size:
941 611 83 42
BstZ17I GTA'TAC
Cuts at: 0 591 598 1677
Size: 591 7 1079
Fragments arranged by size:
1079 591 7
Bsu36I CC'TnA_GG
Cuts at: 0 1304 1677
Size: 1304 373
BtgI C'CryG_G
Cuts at: 0 1094 1677
Size: 1094 583
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 816 1082 1677
Size: 816 266 595
Fragments arranged by size:
816 595 266
BtsI GCAGTG_nn'
Cuts at: 0 1462 1677
Size: 1462 215
DrdI GACnn_nn'nnGTC
Cuts at: 0 118 1677
Size: 118 1559
EaeI y'GGCC_r
Cuts at: 0 1082 1677
Size: 1082 595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 792 1494 1616 1677
Size: 674 118 702 122 61
Fragments arranged by size:
702 674 122 118 61
EcoO109I rG'GnC_Cy
Cuts at: 0 537 1234 1317 1341 1384 1677
Size: 537 697 83 24 43 293
Fragments arranged by size:
697 537 293 83 43 24
EcoRI G'AATT_C
Cuts at: 0 1142 1677
Size: 1142 535
EcoRV GAT'ATC
Cuts at: 0 491 1677
Size: 491 1186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 880 1153 1677
Size: 723 157 273 524
Fragments arranged by size:
723 524 273 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 1319 1333 1365 1509 1541 1677
Size: 1287 32 14 32 144 32 136
Fragments arranged by size:
1287 144 136 32 32 32 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1677
Size: 250 1427
MscI TGG'CCA
Cuts at: 0 1084 1677
Size: 1084 593
MspA1I CmG'CkG
Cuts at: 0 339 757 1677
Size: 339 418 920
Fragments arranged by size:
920 418 339
NaeI GCC'GGC
Cuts at: 0 105 1677
Size: 105 1572
NdeI CA'TA_TG
Cuts at: 0 463 1677
Size: 463 1214
NgoMIV G'CCGG_C
Cuts at: 0 103 1677
Size: 103 1574
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 464 850 1677
Size: 93 371 386 827
Fragments arranged by size:
827 386 371 93
PfoI T'CCnGG_A
Cuts at: 0 808 849 1441 1677
Size: 808 41 592 236
Fragments arranged by size:
808 592 236 41
PpuMI rG'GwC_Cy
Cuts at: 0 537 1234 1341 1677
Size: 537 697 107 336
Fragments arranged by size:
697 537 336 107
PshAI GACnn'nnGTC
Cuts at: 0 96 1677
Size: 96 1581
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 1677
Size: 1170 32 475
Fragments arranged by size:
1170 475 32
PstI C_TGCA'G
Cuts at: 0 1409 1677
Size: 1409 268
PvuII CAG'CTG
Cuts at: 0 339 757 1677
Size: 339 418 920
Fragments arranged by size:
920 418 339
SanDI GG'GwC_CC
Cuts at: 0 537 1341 1677
Size: 537 804 336
Fragments arranged by size:
804 537 336
SfcI C'TryA_G
Cuts at: 0 1405 1568 1677
Size: 1405 163 109
Fragments arranged by size:
1405 163 109
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1315 1677
Size: 1315 362
StyI C'CwwG_G
Cuts at: 0 522 1677
Size: 522 1155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1009 1451 1677
Size: 1009 442 226
Fragments arranged by size:
1009 442 226
TatI w'GTAC_w
Cuts at: 0 47 1677
Size: 47 1630
XmnI GAAnn'nnTTC
Cuts at: 0 703 1577 1677
Size: 703 874 100
Fragments arranged by size:
874 703 100
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI BamHI BanI BanII
BbsI BglI BmrI BplI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsmBI Bsp1286I BsrFI BsrGI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI
EaeI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I MmeI
MscI MspA1I NaeI NdeI NgoMIV PflMI PfoI PpuMI
PshAI PsrI PstI PvuII SanDI SfcI SfiI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaI
BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI