Restriction map for
pFN21AE2270
(Linear) (Six-base) MAPSORT of: KIEE2270.seq Check: 9653 from: 1 to: 438
KIEE2270.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 210 438
Size: 210 228
AflIII A'CryG_T
Cuts at: 0 197 292 438
Size: 197 95 146
Fragments arranged by size:
197 146 95
ApaI G_GGCC'C
Cuts at: 0 11 438
Size: 11 427
ApaLI G'TGCA_C
Cuts at: 0 152 438
Size: 152 286
BanII G_rGCy'C
Cuts at: 0 11 67 438
Size: 11 56 371
Fragments arranged by size:
371 56 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 225 259 438
Size: 225 34 179
Fragments arranged by size:
225 179 34
BglI GCCn_nnn'nGGC
Cuts at: 0 43 438
Size: 43 395
BglII A'GATC_T
Cuts at: 0 377 438
Size: 377 61
Bme1580I G_kGCm'C
Cuts at: 0 11 156 403 438
Size: 11 145 247 35
Fragments arranged by size:
247 145 35 11
BmgBI CAC'GTC
Cuts at: 0 273 438
Size: 273 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 438
Size: 63 375
BsaAI yAC'GTr
Cuts at: 0 293 438
Size: 293 145
BsaHI Gr'CG_yC
Cuts at: 0 122 434 438
Size: 122 312 4
Fragments arranged by size:
312 122 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 151 438
Size: 148 3 287
Fragments arranged by size:
287 148 3
BseYI C'CCAG_C
Cuts at: 0 250 279 305 438
Size: 250 29 26 133
Fragments arranged by size:
250 133 29 26
BsiEI CG_ry'CG
Cuts at: 0 74 438
Size: 74 364
BsiHKAI G_wGCw'C
Cuts at: 0 156 438
Size: 156 282
BsmI GAATG_Cn'
Cuts at: 0 361 438
Size: 361 77
Bsp1286I G_dGCh'C
Cuts at: 0 11 67 156 403 438
Size: 11 56 89 247 35
Fragments arranged by size:
247 89 56 35 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 438
Size: 210 228
BsrFI r'CCGG_y
Cuts at: 0 73 438
Size: 73 365
BssHII G'CGCG_C
Cuts at: 0 15 212 228 438
Size: 15 197 16 210
Fragments arranged by size:
210 197 16 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 225 438
Size: 225 213
BstYI r'GATC_y
Cuts at: 0 377 438
Size: 377 61
BtgI C'CryG_G
Cuts at: 0 47 68 116 438
Size: 47 21 48 322
Fragments arranged by size:
322 48 47 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 166 438
Size: 166 272
EaeI y'GGCC_r
Cuts at: 0 2 35 44 56 71 438
Size: 2 33 9 12 15 367
Fragments arranged by size:
367 33 15 12 9 2
EagI C'GGCC_G
Cuts at: 0 71 438
Size: 71 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 438
Size: 63 375
EcoO109I rG'GnC_Cy
Cuts at: 0 8 112 438
Size: 8 104 326
Fragments arranged by size:
326 104 8
MscI TGG'CCA
Cuts at: 0 37 438
Size: 37 401
MspA1I CmG'CkG
Cuts at: 0 24 70 438
Size: 24 46 368
Fragments arranged by size:
368 46 24
NaeI GCC'GGC
Cuts at: 0 75 438
Size: 75 363
NcoI C'CATG_G
Cuts at: 0 116 438
Size: 116 322
NgoMIV G'CCGG_C
Cuts at: 0 73 438
Size: 73 365
NspI r_CATG'y
Cuts at: 0 201 438
Size: 201 237
PciI A'CATG_T
Cuts at: 0 197 438
Size: 197 241
PmlI CAC'GTG
Cuts at: 0 293 438
Size: 293 145
PspOMI G'GGCC_C
Cuts at: 0 7 438
Size: 7 431
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 279 311 438
Size: 279 32 127
Fragments arranged by size:
279 127 32
PstI C_TGCA'G
Cuts at: 0 221 438
Size: 221 217
SacII CC_GC'GG
Cuts at: 0 71 438
Size: 71 367
SfcI C'TryA_G
Cuts at: 0 217 438
Size: 217 221
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 43 438
Size: 43 395
StyI C'CwwG_G
Cuts at: 0 116 391 438
Size: 116 275 47
Fragments arranged by size:
275 116 47
TatI w'GTAC_w
Cuts at: 0 176 438
Size: 176 262
Tth111I GACn'n_nGTC
Cuts at: 0 411 438
Size: 411 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 438
Size: 45 393
XmnI GAAnn'nnTTC
Cuts at: 0 262 361 438
Size: 262 99 77
Fragments arranged by size:
262 99 77
Enzymes that do cut:
AarI AflIII ApaI ApaLI BanII BcgI BglI BglII
Bme1580I BmgBI BpmI BsaAI BsaHI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrFI BssHII BstAPI BstYI
BtgI DrdI EaeI EagI Eco57MI EcoO109I MscI MspA1I
NaeI NcoI NgoMIV NspI PciI PmlI PspOMI PsrI
PstI SacII SfcI SfiI StyI TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BclI BfrBI BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsgI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI ZraI