(Linear) (Six-base) MAPSORT of: KIEE2269.seq  Check: 5091  from: 1  to: 252



KIEE2269.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

ApoI r'AATT_y

Cuts at:      0       7     150     252 
   Size:          7     143     102

  Fragments arranged by size: 

                 143     102       7
 
BanII G_rGCy'C

Cuts at:      0      95     252 
   Size:         95     157
 
BbvCI CC'TCA_GC

Cuts at:      0      47     252 
   Size:         47     205
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     231     252 
   Size:        231      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0      57     252 
   Size:         57     195
 
Bpu10I CC'TnA_GC

Cuts at:      0      47     252 
   Size:         47     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     125     252 
   Size:        125     127
 
BsmI GAATG_Cn'

Cuts at:      0     212     252 
   Size:        212      40
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     252 
   Size:         95     157
 
BssSI C'ACGA_G

Cuts at:      0     232     252 
   Size:        232      20
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      84     119     252 
   Size:         84      35     133

  Fragments arranged by size: 

                 133      84      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      81     252 
   Size:         81     171
 
EcoRI G'AATT_C

Cuts at:      0       7     150     252 
   Size:          7     143     102

  Fragments arranged by size: 

                 143     102       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     252 
   Size:        102     150
 
StyI C'CwwG_G

Cuts at:      0     185     252 
   Size:        185      67
 
XmnI GAAnn'nnTTC

Cuts at:      0     150     252 
   Size:        150     102
 

 Enzymes that do cut:

     ApoI    BanII    BbvCI    BciVI     BglI   Bpu10I     BsaI     BsmI
 Bsp1286I    BssSI    CspCI     EciI    EcoRI     MmeI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI