Restriction map for
pFN21AE2264
(Linear) (Six-base) MAPSORT of: KIEE2264.seq Check: 6055 from: 1 to: 1002
KIEE2264.orf
With 182 enzymes: SgfI *
March 22, 2012 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 1002
Size: 94 908
AccI GT'mk_AC
Cuts at: 0 836 1002
Size: 836 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 658 893 1002
Size: 442 216 235 109
Fragments arranged by size:
442 235 216 109
AhdI GACnn_n'nnGTC
Cuts at: 0 266 1002
Size: 266 736
ApoI r'AATT_y
Cuts at: 0 898 1002
Size: 898 104
AvaI C'yCGr_G
Cuts at: 0 48 215 866 1002
Size: 48 167 651 136
Fragments arranged by size:
651 167 136 48
BanI G'GyrC_C
Cuts at: 0 82 1002
Size: 82 920
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1002
Size: 265 737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 372 406 1002
Size: 372 34 596
Fragments arranged by size:
596 372 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 297 1002
Size: 297 705
BglII A'GATC_T
Cuts at: 0 333 510 1002
Size: 333 177 492
Fragments arranged by size:
492 333 177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 529 1002
Size: 529 473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 599 943 1002
Size: 336 263 344 59
Fragments arranged by size:
344 336 263 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 512 760 1002
Size: 512 248 242
Fragments arranged by size:
512 248 242
BsaBI GATnn'nnATC
Cuts at: 0 332 1002
Size: 332 670
BsaHI Gr'CG_yC
Cuts at: 0 741 1002
Size: 741 261
BseYI C'CCAG_C
Cuts at: 0 953 1002
Size: 953 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1002
Size: 376 626
BsiEI CG_ry'CG
Cuts at: 0 833 1002
Size: 833 169
BsmI GAATG_Cn'
Cuts at: 0 441 1002
Size: 441 561
BsmBI CGTCTCn'nnnn_
Cuts at: 0 732 1002
Size: 732 270
BspHI T'CATG_A
Cuts at: 0 719 1002
Size: 719 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 740 778 1002
Size: 94 646 38 224
Fragments arranged by size:
646 224 94 38
BsrGI T'GTAC_A
Cuts at: 0 851 1002
Size: 851 151
BssSI C'ACGA_G
Cuts at: 0 381 1002
Size: 381 621
BstAPI GCAn_nnn'nTGC
Cuts at: 0 404 933 1002
Size: 404 529 69
Fragments arranged by size:
529 404 69
BstEII G'GTnAC_C
Cuts at: 0 860 1002
Size: 860 142
BstYI r'GATC_y
Cuts at: 0 333 510 1002
Size: 333 177 492
Fragments arranged by size:
492 333 177
BtgI C'CryG_G
Cuts at: 0 222 1002
Size: 222 780
DraIII CAC_nnn'GTG
Cuts at: 0 162 1002
Size: 162 840
DrdI GACnn_nn'nnGTC
Cuts at: 0 774 1002
Size: 774 228
EaeI y'GGCC_r
Cuts at: 0 2 830 1002
Size: 2 828 172
Fragments arranged by size:
828 172 2
EagI C'GGCC_G
Cuts at: 0 830 1002
Size: 830 172
EarI CTCTTCn'nnn_
Cuts at: 0 530 1002
Size: 530 472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 442 599 658 893 943 1002
Size: 336 106 157 59 235 50 59
Fragments arranged by size:
336 235 157 106 59 59 50
EcoRI G'AATT_C
Cuts at: 0 898 1002
Size: 898 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 1002
Size: 252 32 718
Fragments arranged by size:
718 252 32
HincII GTy'rAC
Cuts at: 0 285 363 837 1002
Size: 285 78 474 165
Fragments arranged by size:
474 285 165 78
MfeI C'AATT_G
Cuts at: 0 568 1002
Size: 568 434
MscI TGG'CCA
Cuts at: 0 4 1002
Size: 4 998
NcoI C'CATG_G
Cuts at: 0 222 1002
Size: 222 780
PflMI CCAn_nnn'nTGG
Cuts at: 0 314 1002
Size: 314 688
PfoI T'CCnGG_A
Cuts at: 0 902 1002
Size: 902 100
PsiI TTA'TAA
Cuts at: 0 241 1002
Size: 241 761
PspXI vC'TCGA_Gb
Cuts at: 0 48 215 866 1002
Size: 48 167 651 136
Fragments arranged by size:
651 167 136 48
SalI G'TCGA_C
Cuts at: 0 835 1002
Size: 835 167
SmlI C'TyrA_G
Cuts at: 0 48 215 866 1002
Size: 48 167 651 136
Fragments arranged by size:
651 167 136 48
SspI AAT'ATT
Cuts at: 0 352 1002
Size: 352 650
StyI C'CwwG_G
Cuts at: 0 222 288 1002
Size: 222 66 714
Fragments arranged by size:
714 222 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 688 803 1002
Size: 688 115 199
Fragments arranged by size:
688 199 115
TatI w'GTAC_w
Cuts at: 0 851 1002
Size: 851 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 884 916 1002
Size: 884 32 86
Fragments arranged by size:
884 86 32
XbaI T'CTAG_A
Cuts at: 0 432 1002
Size: 432 570
XhoI C'TCGA_G
Cuts at: 0 48 215 866 1002
Size: 48 167 651 136
Fragments arranged by size:
651 167 136 48
XmnI GAAnn'nnTTC
Cuts at: 0 335 1002
Size: 335 667
Enzymes that do cut:
AarI AccI AcuI AhdI ApoI AvaI BanI BbsI
BcgI BciVI BglII BplI BpmI BsaI BsaBI BsaHI
BseYI BsgI BsiEI BsmI BsmBI BspHI BspMI BsrGI
BssSI BstAPI BstEII BstYI BtgI DraIII DrdI EaeI
EagI EarI Eco57MI EcoRI Hin4I HincII MfeI MscI
NcoI PflMI PfoI PsiI PspXI SalI SmlI SspI
StyI TaqII TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaAI BsaWI BsaXI BseRI BsiHKAI BsiWI Bsp1286I BspEI
BsrBI BsrDI BsrFI BssHII BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MmeI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XcmI XmaI ZraI