Restriction map for
pFN21AE2263
(Linear) (Six-base) MAPSORT of: KIEE2263.seq Check: 3286 from: 1 to: 1155
KIEE2263.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AccI GT'mk_AC
Cuts at: 0 726 1155
Size: 726 429
AclI AA'CG_TT
Cuts at: 0 377 1155
Size: 377 778
AflIII A'CryG_T
Cuts at: 0 1131 1155
Size: 1131 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 677 854 1155
Size: 677 177 301
Fragments arranged by size:
677 301 177
AlwNI CAG_nnn'CTG
Cuts at: 0 1027 1155
Size: 1027 128
ApaI G_GGCC'C
Cuts at: 0 32 678 1155
Size: 32 646 477
Fragments arranged by size:
646 477 32
ApoI r'AATT_y
Cuts at: 0 274 755 1155
Size: 274 481 400
Fragments arranged by size:
481 400 274
AvaI C'yCGr_G
Cuts at: 0 632 888 1155
Size: 632 256 267
Fragments arranged by size:
632 267 256
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 149 182 1155
Size: 149 33 973
Fragments arranged by size:
973 149 33
BamHI G'GATC_C
Cuts at: 0 812 1155
Size: 812 343
BanII G_rGCy'C
Cuts at: 0 32 678 897 955 1077 1155
Size: 32 646 219 58 122 78
Fragments arranged by size:
646 219 122 78 58 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 983 1155
Size: 983 172
BciVI GTATCCnnnnn_n'
Cuts at: 0 177 1155
Size: 177 978
BglI GCCn_nnn'nGGC
Cuts at: 0 901 1155
Size: 901 254
Bme1580I G_kGCm'C
Cuts at: 0 32 678 1155
Size: 32 646 477
Fragments arranged by size:
646 477 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 1155
Size: 696 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 573 592 969 1089 1155
Size: 371 202 19 377 120 66
Fragments arranged by size:
377 371 202 120 66 19
Bpu10I CC'TnA_GC
Cuts at: 0 1020 1046 1076 1155
Size: 1020 26 30 79
Fragments arranged by size:
1020 79 30 26
BsaAI yAC'GTr
Cuts at: 0 1134 1155
Size: 1134 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 1155
Size: 664 491
BseYI C'CCAG_C
Cuts at: 0 369 670 807 969 1155
Size: 369 301 137 162 186
Fragments arranged by size:
369 301 186 162 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1155
Size: 199 956
Bsp1286I G_dGCh'C
Cuts at: 0 32 678 897 955 1077 1155
Size: 32 646 219 58 122 78
Fragments arranged by size:
646 219 122 78 58 32
BspHI T'CATG_A
Cuts at: 0 89 1155
Size: 89 1066
BsrDI GCAATG_nn'
Cuts at: 0 243 1155
Size: 243 912
BsrFI r'CCGG_y
Cuts at: 0 1090 1155
Size: 1090 65
BssHII G'CGCG_C
Cuts at: 0 17 19 21 1155
Size: 17 2 2 1134
Fragments arranged by size:
1134 17 2 2
BstEII G'GTnAC_C
Cuts at: 0 882 1155
Size: 882 273
BstYI r'GATC_y
Cuts at: 0 812 1155
Size: 812 343
Bsu36I CC'TnA_GG
Cuts at: 0 1040 1155
Size: 1040 115
BtgI C'CryG_G
Cuts at: 0 40 1155
Size: 40 1115
DraIII CAC_nnn'GTG
Cuts at: 0 222 1137 1155
Size: 222 915 18
Fragments arranged by size:
915 222 18
EaeI y'GGCC_r
Cuts at: 0 740 1092 1113 1155
Size: 740 352 21 42
Fragments arranged by size:
740 352 42 21
EarI CTCTTCn'nnn_
Cuts at: 0 327 1155
Size: 327 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 573 592 969 1089 1155
Size: 371 202 19 377 120 66
Fragments arranged by size:
377 371 202 120 66 19
EcoO109I rG'GnC_Cy
Cuts at: 0 28 624 674 1024 1031 1155
Size: 28 596 50 350 7 124
Fragments arranged by size:
596 350 124 50 28 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 1155
Size: 795 360
MscI TGG'CCA
Cuts at: 0 742 1155
Size: 742 413
MslI CAynn'nnrTG
Cuts at: 0 236 1155
Size: 236 919
MspA1I CmG'CkG
Cuts at: 0 42 593 947 1155
Size: 42 551 354 208
Fragments arranged by size:
551 354 208 42
PasI CC'CwG_GG
Cuts at: 0 135 1155
Size: 135 1020
PmlI CAC'GTG
Cuts at: 0 1134 1155
Size: 1134 21
PpuMI rG'GwC_Cy
Cuts at: 0 1024 1031 1155
Size: 1024 7 124
Fragments arranged by size:
1024 124 7
PspOMI G'GGCC_C
Cuts at: 0 28 674 1155
Size: 28 646 481
Fragments arranged by size:
646 481 28
PvuII CAG'CTG
Cuts at: 0 593 947 1155
Size: 593 354 208
Fragments arranged by size:
593 354 208
SacII CC_GC'GG
Cuts at: 0 43 1155
Size: 43 1112
SfcI C'TryA_G
Cuts at: 0 1014 1155
Size: 1014 141
StuI AGG'CCT
Cuts at: 0 717 1155
Size: 717 438
StyI C'CwwG_G
Cuts at: 0 198 1155
Size: 198 957
XmnI GAAnn'nnTTC
Cuts at: 0 144 426 1155
Size: 144 282 729
Fragments arranged by size:
729 282 144
Enzymes that do cut:
AccI AclI AflIII AlfI AlwNI ApaI ApoI AvaI
BaeI BamHI BanII BbsI BciVI BglI Bme1580I BmrI
BpmI Bpu10I BsaAI BseRI BseYI BsgI Bsp1286I BspHI
BsrDI BsrFI BssHII BstEII BstYI Bsu36I BtgI DraIII
EaeI EarI Eco57MI EcoO109I MmeI MscI MslI MspA1I
PasI PmlI PpuMI PspOMI PvuII SacII SfcI StuI
StyI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BanI
BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrGI BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI