Restriction map for
pFN21AE2260
(Linear) (Six-base) MAPSORT of: KIEE2260.seq Check: 6820 from: 1 to: 1551
KIEE2260.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 125 137 1551
Size: 125 12 1414
Fragments arranged by size:
1414 125 12
AccI GT'mk_AC
Cuts at: 0 257 1436 1551
Size: 257 1179 115
Fragments arranged by size:
1179 257 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1472 1475 1551
Size: 945 527 3 76
Fragments arranged by size:
945 527 76 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 1551
Size: 835 716
AlwNI CAG_nnn'CTG
Cuts at: 0 117 129 1212 1480 1551
Size: 117 12 1083 268 71
Fragments arranged by size:
1083 268 117 71 12
ApaLI G'TGCA_C
Cuts at: 0 1084 1551
Size: 1084 467
ApoI r'AATT_y
Cuts at: 0 187 411 1551
Size: 187 224 1140
Fragments arranged by size:
1140 224 187
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1023 1056 1551
Size: 1023 33 495
Fragments arranged by size:
1023 495 33
BbvCI CC'TCA_GC
Cuts at: 0 1526 1551
Size: 1526 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 959 993 1162 1196 1551
Size: 959 34 169 34 355
Fragments arranged by size:
959 355 169 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 903 1551
Size: 903 648
BglI GCCn_nnn'nGGC
Cuts at: 0 1122 1274 1551
Size: 1122 152 277
Fragments arranged by size:
1122 277 152
Bme1580I G_kGCm'C
Cuts at: 0 772 969 1088 1551
Size: 772 197 119 463
Fragments arranged by size:
772 463 197 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 1373 1468 1551
Size: 1373 95 83
Fragments arranged by size:
1373 95 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 583 1551
Size: 583 968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1551
Size: 880 671
Bpu10I CC'TnA_GC
Cuts at: 0 1481 1526 1551
Size: 1481 45 25
Fragments arranged by size:
1481 45 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1551
Size: 199 1352
BsaAI yAC'GTr
Cuts at: 0 1395 1551
Size: 1395 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 1551
Size: 22 30 1499
Fragments arranged by size:
1499 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 163 178 937 1342 1543 1551
Size: 160 3 15 759 405 201 8
Fragments arranged by size:
759 405 201 160 15 8 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 939 1551
Size: 516 423 612
Fragments arranged by size:
612 516 423
BsiHKAI G_wGCw'C
Cuts at: 0 79 404 566 1088 1551
Size: 79 325 162 522 463
Fragments arranged by size:
522 463 325 162 79
Bsp1286I G_dGCh'C
Cuts at: 0 79 404 566 772 969 1088 1551
Size: 79 325 162 206 197 119 463
Fragments arranged by size:
463 325 206 197 162 119 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 137 538 796 1551
Size: 125 12 401 258 755
Fragments arranged by size:
755 401 258 125 12
BsrBI CCG'CTC
Cuts at: 0 723 1551
Size: 723 828
BsrFI r'CCGG_y
Cuts at: 0 842 1551
Size: 842 709
BstAPI GCAn_nnn'nTGC
Cuts at: 0 129 306 1215 1551
Size: 129 177 909 336
Fragments arranged by size:
909 336 177 129
BstYI r'GATC_y
Cuts at: 0 1538 1551
Size: 1538 13
Bsu36I CC'TnA_GG
Cuts at: 0 1119 1551
Size: 1119 432
BtgI C'CryG_G
Cuts at: 0 83 1551
Size: 83 1468
BtsI GCAGTG_nn'
Cuts at: 0 264 1551
Size: 264 1287
DraI TTT'AAA
Cuts at: 0 852 1551
Size: 852 699
DraIII CAC_nnn'GTG
Cuts at: 0 1398 1551
Size: 1398 153
EarI CTCTTCn'nnn_
Cuts at: 0 149 155 1313 1319 1551
Size: 149 6 1158 6 232
Fragments arranged by size:
1158 232 149 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 1551
Size: 238 1313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 945 1472 1475 1551
Size: 880 65 527 3 76
Fragments arranged by size:
880 527 76 65 3
EcoO109I rG'GnC_Cy
Cuts at: 0 59 1551
Size: 59 1492
EcoRI G'AATT_C
Cuts at: 0 187 411 1551
Size: 187 224 1140
Fragments arranged by size:
1140 224 187
EcoRV GAT'ATC
Cuts at: 0 372 1551
Size: 372 1179
FspI TGC'GCA
Cuts at: 0 745 1551
Size: 745 806
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1551
Size: 43 32 1476
Fragments arranged by size:
1476 43 32
HindIII A'AGCT_T
Cuts at: 0 1510 1551
Size: 1510 41
MslI CAynn'nnrTG
Cuts at: 0 131 1046 1154 1393 1551
Size: 131 915 108 239 158
Fragments arranged by size:
915 239 158 131 108
MspA1I CmG'CkG
Cuts at: 0 795 875 1176 1551
Size: 795 80 301 375
Fragments arranged by size:
795 375 301 80
NcoI C'CATG_G
Cuts at: 0 83 1551
Size: 83 1468
NspI r_CATG'y
Cuts at: 0 106 448 621 744 822 1551
Size: 106 342 173 123 78 729
Fragments arranged by size:
729 342 173 123 106 78
PasI CC'CwG_GG
Cuts at: 0 1459 1551
Size: 1459 92
PfoI T'CCnGG_A
Cuts at: 0 338 1551
Size: 338 1213
PmlI CAC'GTG
Cuts at: 0 1395 1551
Size: 1395 156
PpuMI rG'GwC_Cy
Cuts at: 0 59 1551
Size: 59 1492
PstI C_TGCA'G
Cuts at: 0 122 302 1175 1274 1551
Size: 122 180 873 99 277
Fragments arranged by size:
873 277 180 122 99
PvuII CAG'CTG
Cuts at: 0 875 1176 1551
Size: 875 301 375
Fragments arranged by size:
875 375 301
SbfI CC_TGCA'GG
Cuts at: 0 1274 1551
Size: 1274 277
SfcI C'TryA_G
Cuts at: 0 43 118 298 870 933 1171 1270 1551
Size: 43 75 180 572 63 238 99 281
Fragments arranged by size:
572 281 238 180 99 75 63 43
SmlI C'TyrA_G
Cuts at: 0 178 1551
Size: 178 1373
StuI AGG'CCT
Cuts at: 0 304 1551
Size: 304 1247
StyI C'CwwG_G
Cuts at: 0 83 324 1027 1416 1551
Size: 83 241 703 389 135
Fragments arranged by size:
703 389 241 135 83
TatI w'GTAC_w
Cuts at: 0 1005 1551
Size: 1005 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 1551
Size: 22 32 1497
Fragments arranged by size:
1497 32 22
XbaI T'CTAG_A
Cuts at: 0 1284 1551
Size: 1284 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 322 1551
Size: 322 1229
XmnI GAAnn'nnTTC
Cuts at: 0 415 1511 1551
Size: 415 1096 40
Fragments arranged by size:
1096 415 40
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaLI ApoI BaeI
BbvCI BcgI BciVI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstYI Bsu36I BtgI BtsI
DraI DraIII EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI Hin4I HindIII MslI MspA1I NcoI NspI PasI
PfoI PmlI PpuMI PstI PvuII SbfI SfcI SmlI
StuI StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BclI BfrBI BglII
BlpI BmgBI BmtI BsaI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DrdI EaeI EagI EcoICRI EcoNI FalI FseI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XhoI XmaI ZraI