Restriction map for
pFN21AE2255
(Linear) (Six-base) MAPSORT of: KIEE2255.seq Check: 5700 from: 1 to: 1122
KIEE2255.orf
With 182 enzymes: SgfI *
March 22, 2012 15:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 954 1122
Size: 209 745 168
Fragments arranged by size:
745 209 168
AfeI AGC'GCT
Cuts at: 0 401 1122
Size: 401 721
AlwNI CAG_nnn'CTG
Cuts at: 0 596 957 1122
Size: 596 361 165
Fragments arranged by size:
596 361 165
ApaI G_GGCC'C
Cuts at: 0 474 1122
Size: 474 648
ApoI r'AATT_y
Cuts at: 0 100 168 1122
Size: 100 68 954
Fragments arranged by size:
954 100 68
AvaI C'yCGr_G
Cuts at: 0 1067 1117 1122
Size: 1067 50 5
Fragments arranged by size:
1067 50 5
BanI G'GyrC_C
Cuts at: 0 331 448 519 642 828 964 1122
Size: 331 117 71 123 186 136 158
Fragments arranged by size:
331 186 158 136 123 117 71
BanII G_rGCy'C
Cuts at: 0 213 474 947 1122
Size: 213 261 473 175
Fragments arranged by size:
473 261 213 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 525 861 1122
Size: 231 294 336 261
Fragments arranged by size:
336 294 261 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 315 1122
Size: 281 34 807
Fragments arranged by size:
807 281 34
BglI GCCn_nnn'nGGC
Cuts at: 0 301 487 1122
Size: 301 186 635
Fragments arranged by size:
635 301 186
BlpI GC'TnA_GC
Cuts at: 0 403 1122
Size: 403 719
Bme1580I G_kGCm'C
Cuts at: 0 204 336 474 524 647 833 1122
Size: 204 132 138 50 123 186 289
Fragments arranged by size:
289 204 186 138 132 123 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 1122
Size: 429 693
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 352 428 1122
Size: 352 76 694
Fragments arranged by size:
694 352 76
BsaHI Gr'CG_yC
Cuts at: 0 653 1122
Size: 653 469
BsaWI w'CCGG_w
Cuts at: 0 55 1122
Size: 55 1067
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 50 80 263 293 998 1028 1122
Size: 50 30 183 30 705 30 94
Fragments arranged by size:
705 183 94 50 30 30 30
BseYI C'CCAG_C
Cuts at: 0 162 586 600 1010 1122
Size: 162 424 14 410 112
Fragments arranged by size:
424 410 162 112 14
BsiEI CG_ry'CG
Cuts at: 0 985 1122
Size: 985 137
Bsp1286I G_dGCh'C
Cuts at: 0 204 213 336 474 524 647 833 947
Size: 204 9 123 138 50 123 186 114
Cuts at: 947 1122
Size: 175
Fragments arranged by size:
204 186 175 138 123 123 114 50
9
BspEI T'CCGG_A
Cuts at: 0 55 1122
Size: 55 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 680 1026 1122
Size: 581 99 346 96
Fragments arranged by size:
581 346 99 96
BsrBI CCG'CTC
Cuts at: 0 737 1122
Size: 737 385
BstAPI GCAn_nnn'nTGC
Cuts at: 0 905 1122
Size: 905 217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 1122
Size: 789 333
BstYI r'GATC_y
Cuts at: 0 975 1122
Size: 975 147
Bsu36I CC'TnA_GG
Cuts at: 0 112 934 1122
Size: 112 822 188
Fragments arranged by size:
822 188 112
BtgI C'CryG_G
Cuts at: 0 431 1122
Size: 431 691
BtsI GCAGTG_nn'
Cuts at: 0 69 497 1122
Size: 69 428 625
Fragments arranged by size:
625 428 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 724 1122
Size: 235 489 398
Fragments arranged by size:
489 398 235
EaeI y'GGCC_r
Cuts at: 0 356 428 1122
Size: 356 72 694
Fragments arranged by size:
694 356 72
EarI CTCTTCn'nnn_
Cuts at: 0 469 1122
Size: 469 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 1019 1122
Size: 271 748 103
Fragments arranged by size:
748 271 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 954 1122
Size: 209 745 168
Fragments arranged by size:
745 209 168
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1033 1122
Size: 1033 89
EcoO109I rG'GnC_Cy
Cuts at: 0 605 1122
Size: 605 517
HaeII r_GCGC'y
Cuts at: 0 403 1122
Size: 403 719
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 967 999 1122
Size: 257 32 678 32 123
Fragments arranged by size:
678 257 123 32 32
MscI TGG'CCA
Cuts at: 0 430 1122
Size: 430 692
MslI CAynn'nnrTG
Cuts at: 0 751 1122
Size: 751 371
MspA1I CmG'CkG
Cuts at: 0 529 615 696 1122
Size: 529 86 81 426
Fragments arranged by size:
529 426 86 81
NcoI C'CATG_G
Cuts at: 0 431 1122
Size: 431 691
PasI CC'CwG_GG
Cuts at: 0 999 1122
Size: 999 123
PshAI GACnn'nnGTC
Cuts at: 0 865 1122
Size: 865 257
PspOMI G'GGCC_C
Cuts at: 0 470 1122
Size: 470 652
PstI C_TGCA'G
Cuts at: 0 620 695 1122
Size: 620 75 427
Fragments arranged by size:
620 427 75
PvuII CAG'CTG
Cuts at: 0 529 615 1122
Size: 529 86 507
Fragments arranged by size:
529 507 86
ScaI AGT'ACT
Cuts at: 0 843 1122
Size: 843 279
SexAI A'CCwGG_T
Cuts at: 0 824 1122
Size: 824 298
SfcI C'TryA_G
Cuts at: 0 616 691 1122
Size: 616 75 431
Fragments arranged by size:
616 431 75
SmaI CCC'GGG
Cuts at: 0 1069 1119 1122
Size: 1069 50 3
Fragments arranged by size:
1069 50 3
StyI C'CwwG_G
Cuts at: 0 431 647 1122
Size: 431 216 475
Fragments arranged by size:
475 431 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 411 466 1002 1122
Size: 45 366 55 536 120
Fragments arranged by size:
536 366 120 55 45
TatI w'GTAC_w
Cuts at: 0 841 923 1122
Size: 841 82 199
Fragments arranged by size:
841 199 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 263 295 998 1030 1122
Size: 50 32 181 32 703 32 92
Fragments arranged by size:
703 181 92 50 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 1122
Size: 159 963
XmaI C'CCGG_G
Cuts at: 0 1067 1117 1122
Size: 1067 50 5
Fragments arranged by size:
1067 50 5
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BmrI BplI BsaHI
BsaWI BsaXI BseYI BsiEI Bsp1286I BspEI BspMI BsrBI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI
MslI MspA1I NcoI PasI PshAI PspOMI PstI PvuII
ScaI SexAI SfcI SmaI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmnI ZraI