(Linear) (Six-base) MAPSORT of: KIEE2250.seq  Check: 6786  from: 1  to: 510



KIEE2250.orf

 With 182 enzymes: SgfI * 

                        October 27, 2011 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     414     510 
   Size:        414      96
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     317     349     510 
   Size:        317      32     161

  Fragments arranged by size: 

                 317     161      32
 
ApoI r'AATT_y

Cuts at:      0     217     454     510 
   Size:        217     237      56

  Fragments arranged by size: 

                 237     217      56
 
BanI G'GyrC_C

Cuts at:      0     236     510 
   Size:        236     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     510 
   Size:         39     471
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     510 
   Size:        119     391
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     317     347     510 
   Size:         28     289      30     163

  Fragments arranged by size: 

                 289     163      30      28
 
BspHI T'CATG_A

Cuts at:      0     282     359     510 
   Size:        282      77     151

  Fragments arranged by size: 

                 282     151      77
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      70     414     510 
   Size:         70     344      96

  Fragments arranged by size: 

                 344      96      70
 
BtsI GCAGTG_nn'

Cuts at:      0      19     510 
   Size:         19     491
 
DraI TTT'AAA

Cuts at:      0     459     510 
   Size:        459      51
 
DraIII CAC_nnn'GTG

Cuts at:      0     348     510 
   Size:        348     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     510 
   Size:        119     391
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     101     133     510 
   Size:         30      71      32     377

  Fragments arranged by size: 

                 377      71      32      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     510 
   Size:        225     285
 
MslI CAynn'nnrTG

Cuts at:      0     242     376     510 
   Size:        242     134     134

  Fragments arranged by size: 

                 242     134     134
 
MspA1I CmG'CkG

Cuts at:      0      39     476     510 
   Size:         39     437      34

  Fragments arranged by size: 

                 437      39      34
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     348     510 
   Size:        348     162
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     317     349     510 
   Size:        317      32     161

  Fragments arranged by size: 

                 317     161      32
 
PstI C_TGCA'G

Cuts at:      0      14      81     510 
   Size:         14      67     429

  Fragments arranged by size: 

                 429      67      14
 
PvuII CAG'CTG

Cuts at:      0      39     510 
   Size:         39     471
 
SfcI C'TryA_G

Cuts at:      0      10      77     510 
   Size:         10      67     433

  Fragments arranged by size: 

                 433      67      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     254     510 
   Size:        254     256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     326     510 
   Size:        326     184
 

 Enzymes that do cut:

     AarI     AloI     ApoI     BanI     BbsI     BpmI    BsaXI    BspHI
    BspMI     BtsI     DraI   DraIII  Eco57MI    Hin4I     MmeI     MslI
   MspA1I    PflMI     PpiI     PstI    PvuII     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI