Restriction map for
pFN21AE2247
(Linear) (Six-base) MAPSORT of: KIEE2247.seq Check: 9374 from: 1 to: 2676
KIEE2247.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1107 2018 2676
Size: 1107 911 658
Fragments arranged by size:
1107 911 658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 416 2676
Size: 208 208 2260
Fragments arranged by size:
2260 208 208
AflIII A'CryG_T
Cuts at: 0 722 2636 2676
Size: 722 1914 40
Fragments arranged by size:
1914 722 40
AhdI GACnn_n'nnGTC
Cuts at: 0 2445 2637 2676
Size: 2445 192 39
Fragments arranged by size:
2445 192 39
AleI CACnn'nnGTG
Cuts at: 0 2138 2676
Size: 2138 538
AlwNI CAG_nnn'CTG
Cuts at: 0 936 1638 2205 2676
Size: 936 702 567 471
Fragments arranged by size:
936 702 567 471
ApaI G_GGCC'C
Cuts at: 0 1464 1931 2676
Size: 1464 467 745
Fragments arranged by size:
1464 745 467
ApaLI G'TGCA_C
Cuts at: 0 259 1612 2676
Size: 259 1353 1064
Fragments arranged by size:
1353 1064 259
ApoI r'AATT_y
Cuts at: 0 2371 2676
Size: 2371 305
AvaI C'yCGr_G
Cuts at: 0 562 904 2210 2302 2676
Size: 562 342 1306 92 374
Fragments arranged by size:
1306 562 374 342 92
BamHI G'GATC_C
Cuts at: 0 866 1934 2676
Size: 866 1068 742
Fragments arranged by size:
1068 866 742
BanI G'GyrC_C
Cuts at: 0 900 959 1762 1830 2676
Size: 900 59 803 68 846
Fragments arranged by size:
900 846 803 68 59
BanII G_rGCy'C
Cuts at: 0 756 1382 1464 1911 1931 2676
Size: 756 626 82 447 20 745
Fragments arranged by size:
756 745 626 447 82 20
BbeI G_GCGC'C
Cuts at: 0 963 2676
Size: 963 1713
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 836 2676
Size: 680 156 1840
Fragments arranged by size:
1840 680 156
BclI T'GATC_A
Cuts at: 0 2055 2676
Size: 2055 621
BfrBI ATG'CAT
Cuts at: 0 918 1980 2676
Size: 918 1062 696
Fragments arranged by size:
1062 918 696
BglI GCCn_nnn'nGGC
Cuts at: 0 760 1549 2676
Size: 760 789 1127
Fragments arranged by size:
1127 789 760
BglII A'GATC_T
Cuts at: 0 266 1433 2676
Size: 266 1167 1243
Fragments arranged by size:
1243 1167 266
BlpI GC'TnA_GC
Cuts at: 0 1039 2002 2676
Size: 1039 963 674
Fragments arranged by size:
1039 963 674
Bme1580I G_kGCm'C
Cuts at: 0 263 905 1464 1616 1765 1931 2676
Size: 263 642 559 152 149 166 745
Fragments arranged by size:
745 642 559 263 166 152 149
BmgBI CAC'GTC
Cuts at: 0 630 1749 2676
Size: 630 1119 927
Fragments arranged by size:
1119 927 630
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 1403 2305 2676
Size: 63 1340 902 371
Fragments arranged by size:
1340 902 371 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 2676
Size: 231 2445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 770 910 958 1021 1132 1186 1510
Size: 226 544 140 48 63 111 54 324
Cuts at: 1510 1531 2011 2083 2676
Size: 21 480 72 593
Fragments arranged by size:
593 544 480 324 226 140 111 72
63 54 48 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 2676
Size: 1285 1391
BsaI GGTCTCn'nnnn_
Cuts at: 0 1086 1261 1507 2676
Size: 1086 175 246 1169
Fragments arranged by size:
1169 1086 246 175
BsaAI yAC'GTr
Cuts at: 0 723 1818 2676
Size: 723 1095 858
Fragments arranged by size:
1095 858 723
BsaHI Gr'CG_yC
Cuts at: 0 311 960 2676
Size: 311 649 1716
Fragments arranged by size:
1716 649 311
BsaWI w'CCGG_w
Cuts at: 0 134 696 1157 2676
Size: 134 562 461 1519
Fragments arranged by size:
1519 562 461 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 2676
Size: 253 30 2393
Fragments arranged by size:
2393 253 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1150 1639 1873 1966 2320 2676
Size: 1150 489 234 93 354 356
Fragments arranged by size:
1150 489 356 354 234 93
BseYI C'CCAG_C
Cuts at: 0 2147 2509 2676
Size: 2147 362 167
Fragments arranged by size:
2147 362 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1003 1538 2676
Size: 949 54 535 1138
Fragments arranged by size:
1138 949 535 54
BsiEI CG_ry'CG
Cuts at: 0 697 2676
Size: 697 1979
BsiHKAI G_wGCw'C
Cuts at: 0 263 1382 1616 2135 2628 2676
Size: 263 1119 234 519 493 48
Fragments arranged by size:
1119 519 493 263 234 48
Bsp1286I G_dGCh'C
Cuts at: 0 263 756 905 1382 1464 1616 1765 1911
Size: 263 493 149 477 82 152 149 146
Cuts at: 1911 1931 2135 2628 2676
Size: 20 204 493 48
Fragments arranged by size:
493 493 477 263 204 152 149 149
146 82 48 20
BspEI T'CCGG_A
Cuts at: 0 134 1157 2676
Size: 134 1023 1519
Fragments arranged by size:
1519 1023 134
BspHI T'CATG_A
Cuts at: 0 713 2384 2676
Size: 713 1671 292
Fragments arranged by size:
1671 713 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 1107 1601 2018 2676
Size: 74 1033 494 417 658
Fragments arranged by size:
1033 658 494 417 74
BsrBI CCG'CTC
Cuts at: 0 1494 2676
Size: 1494 1182
BsrFI r'CCGG_y
Cuts at: 0 557 956 1046 2602 2676
Size: 557 399 90 1556 74
Fragments arranged by size:
1556 557 399 90 74
BssSI C'ACGA_G
Cuts at: 0 1216 2676
Size: 1216 1460
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 1830 2374 2676
Size: 924 906 544 302
Fragments arranged by size:
924 906 544 302
BstXI CCAn_nnnn'nTGG
Cuts at: 0 663 2409 2676
Size: 663 1746 267
Fragments arranged by size:
1746 663 267
BstYI r'GATC_y
Cuts at: 0 266 866 1154 1433 1628 1934 1964 2201
Size: 266 600 288 279 195 306 30 237
Cuts at: 2201 2501 2676
Size: 300 175
Fragments arranged by size:
600 306 300 288 279 266 237 195
175 30
Bsu36I CC'TnA_GG
Cuts at: 0 1790 2676
Size: 1790 886
BtgI C'CryG_G
Cuts at: 0 419 883 1583 2126 2531 2648 2676
Size: 419 464 700 543 405 117 28
Fragments arranged by size:
700 543 464 419 405 117 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1353 2154 2565 2676
Size: 1353 801 411 111
Fragments arranged by size:
1353 801 411 111
DraIII CAC_nnn'GTG
Cuts at: 0 1411 2676
Size: 1411 1265
DrdI GACnn_nn'nnGTC
Cuts at: 0 723 2676
Size: 723 1953
EaeI y'GGCC_r
Cuts at: 0 230 797 845 1697 2676
Size: 230 567 48 852 979
Fragments arranged by size:
979 852 567 230 48
EarI CTCTTCn'nnn_
Cuts at: 0 41 161 419 2020 2676
Size: 41 120 258 1601 656
Fragments arranged by size:
1601 656 258 120 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 778 859 2676
Size: 778 81 1817
Fragments arranged by size:
1817 778 81
EcoICRI GAG'CTC
Cuts at: 0 1380 2676
Size: 1380 1296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 226 416 770 910 958 1021 1132
Size: 208 18 190 354 140 48 63 111
Cuts at: 1132 1186 1510 1531 2011 2083 2676
Size: 54 324 21 480 72 593
Fragments arranged by size:
593 480 354 324 208 190 140 111
72 63 54 48 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 1114 2676
Size: 136 978 1562
Fragments arranged by size:
1562 978 136
EcoO109I rG'GnC_Cy
Cuts at: 0 63 285 1293 1460 1461 1590 1928 1985
Size: 63 222 1008 167 1 129 338 57
Cuts at: 1985 2299 2676
Size: 314 377
Fragments arranged by size:
1008 377 338 314 222 167 129 63
57 1
HaeII r_GCGC'y
Cuts at: 0 963 1695 2095 2656 2676
Size: 963 732 400 561 20
Fragments arranged by size:
963 732 561 400 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2431 2463 2623 2655 2676
Size: 2431 32 160 32 21
Fragments arranged by size:
2431 160 32 32 21
HincII GTy'rAC
Cuts at: 0 279 492 804 1416 2037 2676
Size: 279 213 312 612 621 639
Fragments arranged by size:
639 621 612 312 279 213
KasI G'GCGC_C
Cuts at: 0 959 2676
Size: 959 1717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 663 839 2676
Size: 296 367 176 1837
Fragments arranged by size:
1837 367 296 176
MscI TGG'CCA
Cuts at: 0 232 847 1699 2676
Size: 232 615 852 977
Fragments arranged by size:
977 852 615 232
MslI CAynn'nnrTG
Cuts at: 0 418 1975 2138 2140 2530 2676
Size: 418 1557 163 2 390 146
Fragments arranged by size:
1557 418 390 163 146 2
MspA1I CmG'CkG
Cuts at: 0 999 1527 1827 2061 2157 2676
Size: 999 528 300 234 96 519
Fragments arranged by size:
999 528 519 300 234 96
NarI GG'CG_CC
Cuts at: 0 960 2676
Size: 960 1716
NcoI C'CATG_G
Cuts at: 0 1583 2126 2531 2676
Size: 1583 543 405 145
Fragments arranged by size:
1583 543 405 145
NsiI A_TGCA'T
Cuts at: 0 920 1982 2676
Size: 920 1062 694
Fragments arranged by size:
1062 920 694
NspI r_CATG'y
Cuts at: 0 39 922 1867 2640 2676
Size: 39 883 945 773 36
Fragments arranged by size:
945 883 773 39 36
PasI CC'CwG_GG
Cuts at: 0 369 2358 2676
Size: 369 1989 318
Fragments arranged by size:
1989 369 318
PciI A'CATG_T
Cuts at: 0 2636 2676
Size: 2636 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 2487 2676
Size: 2487 189
PfoI T'CCnGG_A
Cuts at: 0 71 176 407 947 2084 2676
Size: 71 105 231 540 1137 592
Fragments arranged by size:
1137 592 540 231 105 71
PmlI CAC'GTG
Cuts at: 0 1818 2676
Size: 1818 858
PpuMI rG'GwC_Cy
Cuts at: 0 63 1590 1985 2299 2676
Size: 63 1527 395 314 377
Fragments arranged by size:
1527 395 377 314 63
PspOMI G'GGCC_C
Cuts at: 0 1460 1927 2676
Size: 1460 467 749
Fragments arranged by size:
1460 749 467
PstI C_TGCA'G
Cuts at: 0 1025 1598 2676
Size: 1025 573 1078
Fragments arranged by size:
1078 1025 573
PvuII CAG'CTG
Cuts at: 0 999 1527 1827 2061 2157 2676
Size: 999 528 300 234 96 519
Fragments arranged by size:
999 528 519 300 234 96
RsrII CG'GwC_CG
Cuts at: 0 1496 2676
Size: 1496 1180
SacI G_AGCT'C
Cuts at: 0 1382 2676
Size: 1382 1294
SanDI GG'GwC_CC
Cuts at: 0 1985 2299 2676
Size: 1985 314 377
Fragments arranged by size:
1985 377 314
SapI GCTCTTCn'nnn_
Cuts at: 0 2020 2676
Size: 2020 656
SbfI CC_TGCA'GG
Cuts at: 0 1598 2676
Size: 1598 1078
ScaI AGT'ACT
Cuts at: 0 2575 2676
Size: 2575 101
SfcI C'TryA_G
Cuts at: 0 1021 1594 2676
Size: 1021 573 1082
Fragments arranged by size:
1082 1021 573
SfoI GGC'GCC
Cuts at: 0 961 2676
Size: 961 1715
SmaI CCC'GGG
Cuts at: 0 2212 2676
Size: 2212 464
SmlI C'TyrA_G
Cuts at: 0 1300 2676
Size: 1300 1376
SphI G_CATG'C
Cuts at: 0 922 1867 2676
Size: 922 945 809
Fragments arranged by size:
945 922 809
StuI AGG'CCT
Cuts at: 0 1207 1222 1315 2431 2676
Size: 1207 15 93 1116 245
Fragments arranged by size:
1207 1116 245 93 15
StyI C'CwwG_G
Cuts at: 0 437 977 1583 2126 2219 2531 2676
Size: 437 540 606 543 93 312 145
Fragments arranged by size:
606 543 540 437 312 145 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1422 1513 2676
Size: 1422 91 1163
Fragments arranged by size:
1422 1163 91
TatI w'GTAC_w
Cuts at: 0 1529 2573 2676
Size: 1529 1044 103
Fragments arranged by size:
1529 1044 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 2283 2315 2676
Size: 253 32 1998 32 361
Fragments arranged by size:
1998 361 253 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2482 2676
Size: 2482 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 50 745 855 2124 2676
Size: 50 695 110 1269 552
Fragments arranged by size:
1269 695 552 110 50
XmaI C'CCGG_G
Cuts at: 0 2210 2676
Size: 2210 466
XmnI GAAnn'nnTTC
Cuts at: 0 2248 2676
Size: 2248 428
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII RsrII
SacI SanDI SapI SbfI ScaI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BmtI Bpu10I BsaBI BsiWI BsmI BsmBI BsrDI
BsrGI BssHII BstBI BstEII BstZ17I BtsI ClaI CspCI
DraI EagI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI PacI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI SacII SalI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
ZraI