(Linear) (Six-base) MAPSORT of: KIEE2244.seq  Check: 3171  from: 1  to: 756



KIEE2244.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     756 
   Size:          6     750
 
AflII C'TTAA_G

Cuts at:      0     184     756 
   Size:        184     572
 
AflIII A'CryG_T

Cuts at:      0     348     756 
   Size:        348     408
 
AgeI A'CCGG_T

Cuts at:      0       9     756 
   Size:          9     747
 
BanI G'GyrC_C

Cuts at:      0      12     756 
   Size:         12     744
 
BanII G_rGCy'C

Cuts at:      0     285     756 
   Size:        285     471
 
Bme1580I G_kGCm'C

Cuts at:      0      17     756 
   Size:         17     739
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     756 
   Size:        202     554
 
BsaAI yAC'GTr

Cuts at:      0     349     756 
   Size:        349     407
 
BsaHI Gr'CG_yC

Cuts at:      0     685     756 
   Size:        685      71
 
BsaWI w'CCGG_w

Cuts at:      0       9     756 
   Size:          9     747
 
BseYI C'CCAG_C

Cuts at:      0     212     756 
   Size:        212     544
 
BsiHKAI G_wGCw'C

Cuts at:      0     285     756 
   Size:        285     471
 
BsmI GAATG_Cn'

Cuts at:      0     445     756 
   Size:        445     311
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     285     756 
   Size:         17     268     471

  Fragments arranged by size: 

                 471     268      17
 
BsrFI r'CCGG_y

Cuts at:      0       9     756 
   Size:          9     747
 
BsrGI T'GTAC_A

Cuts at:      0     497     756 
   Size:        497     259
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     159     756 
   Size:        159     597
 
BstEII G'GTnAC_C

Cuts at:      0     406     756 
   Size:        406     350
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     615     756 
   Size:        615     141
 
BstYI r'GATC_y

Cuts at:      0      63     756 
   Size:         63     693
 
BtsI GCAGTG_nn'

Cuts at:      0     169     756 
   Size:        169     587
 
DraI TTT'AAA

Cuts at:      0     450     756 
   Size:        450     306
 
EarI CTCTTCn'nnn_

Cuts at:      0      25      73     328     756 
   Size:         25      48     255     428

  Fragments arranged by size: 

                 428     255      48      25
 
EcoICRI GAG'CTC

Cuts at:      0     283     756 
   Size:        283     473
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     756 
   Size:          6     750
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84     756 
   Size:         84     672
 
HincII GTy'rAC

Cuts at:      0     738     756 
   Size:        738      18
 
MslI CAynn'nnrTG

Cuts at:      0     275     337     756 
   Size:        275      62     419

  Fragments arranged by size: 

                 419     275      62
 
NdeI CA'TA_TG

Cuts at:      0     155     756 
   Size:        155     601
 
PstI C_TGCA'G

Cuts at:      0     164     756 
   Size:        164     592
 
SacI G_AGCT'C

Cuts at:      0     285     756 
   Size:        285     471
 
SexAI A'CCwGG_T

Cuts at:      0     232     756 
   Size:        232     524
 
SfcI C'TryA_G

Cuts at:      0     160     756 
   Size:        160     596
 
SmlI C'TyrA_G

Cuts at:      0     184     217     756 
   Size:        184      33     539

  Fragments arranged by size: 

                 539     184      33
 
StyI C'CwwG_G

Cuts at:      0     296     756 
   Size:        296     460
 
TatI w'GTAC_w

Cuts at:      0     497     756 
   Size:        497     259
 
XmnI GAAnn'nnTTC

Cuts at:      0     136     756 
   Size:        136     620
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AgeI     BanI    BanII Bme1580I    BpuEI
    BsaAI    BsaHI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI     BtsI     DraI     EarI
  EcoICRI  Eco57MI     FalI   HincII     MslI     NdeI     PstI     SacI
    SexAI     SfcI     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI