(Linear) (Six-base) MAPSORT of: KIEE2239.seq  Check: 6012  from: 1  to: 1299



KIEE2239.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

Acc65I G'GTAC_C

Cuts at:      0     255    1299 
   Size:        255    1044
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1136    1299 
   Size:        188     948     163

  Fragments arranged by size: 

                 948     188     163
 
AlwNI CAG_nnn'CTG

Cuts at:      0     211     283     307     319    1146    1299 
   Size:        211      72      24      12     827     153

  Fragments arranged by size: 

                 827     211     153      72      24      12
 
ApaLI G'TGCA_C

Cuts at:      0    1009    1299 
   Size:       1009     290
 
ApoI r'AATT_y

Cuts at:      0     643    1061    1254    1299 
   Size:        643     418     193      45

  Fragments arranged by size: 

                 643     418     193      45
 
AseI AT'TA_AT

Cuts at:      0      32    1299 
   Size:         32    1267
 
BanI G'GyrC_C

Cuts at:      0     255     267    1299 
   Size:        255      12    1032

  Fragments arranged by size: 

                1032     255      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     425    1299 
   Size:        425     874
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     898     932    1299 
   Size:        898      34     367

  Fragments arranged by size: 

                 898     367      34
 
BlpI GC'TnA_GC

Cuts at:      0    1050    1299 
   Size:       1050     249
 
Bme1580I G_kGCm'C

Cuts at:      0     408     719    1013    1299 
   Size:        408     311     294     286

  Fragments arranged by size: 

                 408     311     294     286
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     586    1299 
   Size:        586     713
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1299 
   Size:        773     526
 
Bpu10I CC'TnA_GC

Cuts at:      0     308     320     350    1299 
   Size:        308      12      30     949

  Fragments arranged by size: 

                 949     308      30      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562    1299 
   Size:        562     737
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     655    1299 
   Size:        655     644
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     360     390     652     682    1299 
   Size:        360      30     262      30     617

  Fragments arranged by size: 

                 617     360     262      30      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1244    1299 
   Size:       1244      55
 
BsiHKAI G_wGCw'C

Cuts at:      0    1013    1299 
   Size:       1013     286
 
Bsp1286I G_dGCh'C

Cuts at:      0     408     719    1013    1299 
   Size:        408     311     294     286

  Fragments arranged by size: 

                 408     311     294     286
 
BsrGI T'GTAC_A

Cuts at:      0     668    1299 
   Size:        668     631
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1287    1299 
   Size:       1287      12
 
BtsI GCAGTG_nn'

Cuts at:      0     746    1299 
   Size:        746     553
 
DraI TTT'AAA

Cuts at:      0     878    1299 
   Size:        878     421
 
EaeI y'GGCC_r

Cuts at:      0     965    1299 
   Size:        965     334
 
EarI CTCTTCn'nnn_

Cuts at:      0     542    1130    1299 
   Size:        542     588     169

  Fragments arranged by size: 

                 588     542     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     773    1136    1299 
   Size:        188     585     363     163

  Fragments arranged by size: 

                 585     363     188     163
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     427    1299 
   Size:        427     872
 
EcoRI G'AATT_C

Cuts at:      0    1254    1299 
   Size:       1254      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     358    1141    1299 
   Size:        358     783     158

  Fragments arranged by size: 

                 783     358     158
 
HaeII r_GCGC'y

Cuts at:      0       7    1299 
   Size:          7    1292
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390    1074    1106    1224    1256    1299 
   Size:        358      32     684      32     118      32      43

  Fragments arranged by size: 

                 684     358     118      43      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     259    1299 
   Size:        259    1040
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1299 
   Size:        974     325
 
MscI TGG'CCA

Cuts at:      0     967    1299 
   Size:        967     332
 
MslI CAynn'nnrTG

Cuts at:      0     399     670     751    1299 
   Size:        399     271      81     548

  Fragments arranged by size: 

                 548     399     271      81
 
MspA1I CmG'CkG

Cuts at:      0     735    1251    1299 
   Size:        735     516      48

  Fragments arranged by size: 

                 735     516      48
 
NspI r_CATG'y

Cuts at:      0    1228    1299 
   Size:       1228      71
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     796    1299 
   Size:        796     503
 
PshAI GACnn'nnGTC

Cuts at:      0     425    1299 
   Size:        425     874
 
PstI C_TGCA'G

Cuts at:      0     170     414     734    1299 
   Size:        170     244     320     565

  Fragments arranged by size: 

                 565     320     244     170
 
PvuII CAG'CTG

Cuts at:      0     735    1251    1299 
   Size:        735     516      48

  Fragments arranged by size: 

                 735     516      48
 
SapI GCTCTTCn'nnn_

Cuts at:      0     542    1299 
   Size:        542     757
 
SfcI C'TryA_G

Cuts at:      0     166     410     730    1299 
   Size:        166     244     320     569

  Fragments arranged by size: 

                 569     320     244     166
 
SmlI C'TyrA_G

Cuts at:      0     541    1299 
   Size:        541     758
 
SpeI A'CTAG_T

Cuts at:      0    1263    1299 
   Size:       1263      36
 
SphI G_CATG'C

Cuts at:      0    1228    1299 
   Size:       1228      71
 
StyI C'CwwG_G

Cuts at:      0    1280    1299 
   Size:       1280      19
 
TatI w'GTAC_w

Cuts at:      0     627     668    1299 
   Size:        627      41     631

  Fragments arranged by size: 

                 631     627      41
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     975    1299 
   Size:        975     324
 
XmnI GAAnn'nnTTC

Cuts at:      0    1124    1299 
   Size:       1124     175
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI    ApaLI     ApoI     AseI     BanI     BbsI
     BcgI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI     BsgI  BsiHKAI Bsp1286I    BsrGI    BstXI     BtsI     DraI
     EaeI     EarI  Eco57MI    EcoNI    EcoRI     FalI    HaeII    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     NspI    PflMI    PshAI
     PstI    PvuII     SapI     SfcI     SmlI     SpeI     SphI     StyI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI