(Linear) (Six-base) MAPSORT of: KIEE2235.seq  Check: 3590  from: 1  to: 306



KIEE2235.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AccI GT'mk_AC

Cuts at:      0     302     306 
   Size:        302       4
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     194     226     306 
   Size:        194      32      80

  Fragments arranged by size: 

                 194      80      32
 
BanI G'GyrC_C

Cuts at:      0     232     306 
   Size:        232      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     306 
   Size:         95     211
 
BsaAI yAC'GTr

Cuts at:      0     300     306 
   Size:        300       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     194     224     306 
   Size:        194      30      82

  Fragments arranged by size: 

                 194      82      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     180     306 
   Size:        126      54     126

  Fragments arranged by size: 

                 126     126      54
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     226     306 
   Size:        226      80
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     194     226     306 
   Size:        194      32      80

  Fragments arranged by size: 

                 194      80      32
 
SmlI C'TyrA_G

Cuts at:      0     110     306 
   Size:        110     196
 
SnaBI TAC'GTA

Cuts at:      0     300     306 
   Size:        300       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      56     306 
   Size:         56     250
 
XbaI T'CTAG_A

Cuts at:      0     246     306 
   Size:        246      60
 

 Enzymes that do cut:

     AccI     AloI     BanI    BpuEI    BsaAI    BsaXI     MmeI    PflMI
     PpiI     SmlI    SnaBI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI