Restriction map for
pFN21AE2234
(Linear) (Six-base) MAPSORT of: KIEE2234.seq Check: 7113 from: 1 to: 789
KIEE2234.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 43 789
Size: 43 746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 670 789
Size: 265 405 119
Fragments arranged by size:
405 265 119
AfeI AGC'GCT
Cuts at: 0 265 789
Size: 265 524
AleI CACnn'nnGTG
Cuts at: 0 479 574 789
Size: 479 95 215
Fragments arranged by size:
479 215 95
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 789
Size: 509 280
AlwNI CAG_nnn'CTG
Cuts at: 0 648 789
Size: 648 141
ApoI r'AATT_y
Cuts at: 0 193 789
Size: 193 596
BamHI G'GATC_C
Cuts at: 0 162 789
Size: 162 627
BanII G_rGCy'C
Cuts at: 0 32 597 605 789
Size: 32 565 8 184
Fragments arranged by size:
565 184 32 8
BclI T'GATC_A
Cuts at: 0 2 710 789
Size: 2 708 79
Fragments arranged by size:
708 79 2
BlpI GC'TnA_GC
Cuts at: 0 31 789
Size: 31 758
Bpu10I CC'TnA_GC
Cuts at: 0 599 789
Size: 599 190
BsaAI yAC'GTr
Cuts at: 0 443 789
Size: 443 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 789
Size: 115 30 644
Fragments arranged by size:
644 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 737 789
Size: 184 553 52
Fragments arranged by size:
553 184 52
BseYI C'CCAG_C
Cuts at: 0 24 100 158 302 497 789
Size: 24 76 58 144 195 292
Fragments arranged by size:
292 195 144 76 58 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 789
Size: 27 762
BsiHKAI G_wGCw'C
Cuts at: 0 587 789
Size: 587 202
Bsp1286I G_dGCh'C
Cuts at: 0 32 587 597 605 789
Size: 32 555 10 8 184
Fragments arranged by size:
555 184 32 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 43 163 789
Size: 43 120 626
Fragments arranged by size:
626 120 43
BsrDI GCAATG_nn'
Cuts at: 0 279 789
Size: 279 510
BssSI C'ACGA_G
Cuts at: 0 378 789
Size: 378 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 267 789
Size: 267 522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 505 789
Size: 505 284
BstYI r'GATC_y
Cuts at: 0 144 162 789
Size: 144 18 627
Fragments arranged by size:
627 144 18
BtsI GCAGTG_nn'
Cuts at: 0 486 674 789
Size: 486 188 115
Fragments arranged by size:
486 188 115
EaeI y'GGCC_r
Cuts at: 0 337 729 789
Size: 337 392 60
Fragments arranged by size:
392 337 60
EarI CTCTTCn'nnn_
Cuts at: 0 755 789
Size: 755 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 735 789
Size: 735 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 670 789
Size: 265 405 119
Fragments arranged by size:
405 265 119
FspI TGC'GCA
Cuts at: 0 271 485 789
Size: 271 214 304
Fragments arranged by size:
304 271 214
FspAI rTGC'GCAy
Cuts at: 0 485 789
Size: 485 304
HaeII r_GCGC'y
Cuts at: 0 267 789
Size: 267 522
HindIII A'AGCT_T
Cuts at: 0 460 789
Size: 460 329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 789
Size: 71 718
MscI TGG'CCA
Cuts at: 0 731 789
Size: 731 58
MslI CAynn'nnrTG
Cuts at: 0 256 479 574 789
Size: 256 223 95 215
Fragments arranged by size:
256 223 215 95
MspA1I CmG'CkG
Cuts at: 0 18 716 789
Size: 18 698 73
Fragments arranged by size:
698 73 18
PmlI CAC'GTG
Cuts at: 0 443 789
Size: 443 346
PstI C_TGCA'G
Cuts at: 0 669 789
Size: 669 120
PvuII CAG'CTG
Cuts at: 0 716 789
Size: 716 73
SfcI C'TryA_G
Cuts at: 0 665 789
Size: 665 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 789
Size: 316 473
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 735 767 789
Size: 735 32 22
Fragments arranged by size:
735 32 22
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApoI BamHI
BanII BclI BlpI Bpu10I BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI
BstYI BtsI EaeI EarI EciI Eco57MI FspI FspAI
HaeII HindIII MmeI MscI MslI MspA1I PmlI PstI
PvuII SfcI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI Hin4I HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI