Restriction map for
pFN21AE2233
(Linear) (Six-base) MAPSORT of: KIEE2233.seq Check: 9063 from: 1 to: 1986
KIEE2233.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AflII C'TTAA_G
Cuts at: 0 271 1986
Size: 271 1715
AflIII A'CryG_T
Cuts at: 0 1562 1986
Size: 1562 424
AleI CACnn'nnGTG
Cuts at: 0 1067 1986
Size: 1067 919
AlwNI CAG_nnn'CTG
Cuts at: 0 928 1986
Size: 928 1058
ApaI G_GGCC'C
Cuts at: 0 17 61 1986
Size: 17 44 1925
Fragments arranged by size:
1925 44 17
ApoI r'AATT_y
Cuts at: 0 457 1546 1677 1986
Size: 457 1089 131 309
Fragments arranged by size:
1089 457 309 131
AvaI C'yCGr_G
Cuts at: 0 54 1986
Size: 54 1932
BamHI G'GATC_C
Cuts at: 0 1005 1716 1834 1986
Size: 1005 711 118 152
Fragments arranged by size:
1005 711 152 118
BanII G_rGCy'C
Cuts at: 0 17 61 541 1986
Size: 17 44 480 1445
Fragments arranged by size:
1445 480 44 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 1986
Size: 115 1871
BclI T'GATC_A
Cuts at: 0 1956 1986
Size: 1956 30
BglII A'GATC_T
Cuts at: 0 322 1986
Size: 322 1664
BlpI GC'TnA_GC
Cuts at: 0 548 633 1986
Size: 548 85 1353
Fragments arranged by size:
1353 548 85
Bme1580I G_kGCm'C
Cuts at: 0 17 61 735 1986
Size: 17 44 674 1251
Fragments arranged by size:
1251 674 44 17
BmtI G_CTAG'C
Cuts at: 0 1903 1986
Size: 1903 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 573 1986
Size: 129 444 1413
Fragments arranged by size:
1413 444 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 905 1986
Size: 208 697 1081
Fragments arranged by size:
1081 697 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1986
Size: 329 1657
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 1986
Size: 343 1643
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 1986
Size: 226 30 1730
Fragments arranged by size:
1730 226 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1553 1986
Size: 1553 433
BsiHKAI G_wGCw'C
Cuts at: 0 513 541 1064 1986
Size: 513 28 523 922
Fragments arranged by size:
922 523 513 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1640 1986
Size: 1640 346
Bsp1286I G_dGCh'C
Cuts at: 0 17 61 513 541 735 1064 1986
Size: 17 44 452 28 194 329 922
Fragments arranged by size:
922 452 329 194 44 28 17
BsrBI CCG'CTC
Cuts at: 0 301 1986
Size: 301 1685
BsrDI GCAATG_nn'
Cuts at: 0 962 1055 1986
Size: 962 93 931
Fragments arranged by size:
962 931 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 220 1986
Size: 220 1766
BstEII G'GTnAC_C
Cuts at: 0 310 1986
Size: 310 1676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1279 1986
Size: 1279 707
BstYI r'GATC_y
Cuts at: 0 322 1005 1716 1834 1986
Size: 322 683 711 118 152
Fragments arranged by size:
711 683 322 152 118
BtgI C'CryG_G
Cuts at: 0 782 1457 1986
Size: 782 675 529
Fragments arranged by size:
782 675 529
BtsI GCAGTG_nn'
Cuts at: 0 899 1211 1986
Size: 899 312 775
Fragments arranged by size:
899 775 312
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 1986
Size: 400 1586
EaeI y'GGCC_r
Cuts at: 0 755 1986
Size: 755 1231
EarI CTCTTCn'nnn_
Cuts at: 0 1156 1761 1889 1986
Size: 1156 605 128 97
Fragments arranged by size:
1156 605 128 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 1986
Size: 96 1890
EcoICRI GAG'CTC
Cuts at: 0 539 1986
Size: 539 1447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 905 1986
Size: 208 697 1081
Fragments arranged by size:
1081 697 208
EcoO109I rG'GnC_Cy
Cuts at: 0 14 58 1986
Size: 14 44 1928
Fragments arranged by size:
1928 44 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 340 372 616 648 1708 1740
Size: 109 32 199 32 244 32 1060 32
Cuts at: 1740 1986
Size: 246
Fragments arranged by size:
1060 246 244 199 109 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 134 1986
Size: 134 1852
MscI TGG'CCA
Cuts at: 0 757 1986
Size: 757 1229
MslI CAynn'nnrTG
Cuts at: 0 308 1067 1986
Size: 308 759 919
Fragments arranged by size:
919 759 308
NcoI C'CATG_G
Cuts at: 0 782 1457 1986
Size: 782 675 529
Fragments arranged by size:
782 675 529
NdeI CA'TA_TG
Cuts at: 0 1743 1986
Size: 1743 243
NheI G'CTAG_C
Cuts at: 0 1899 1986
Size: 1899 87
NruI TCG'CGA
Cuts at: 0 501 1986
Size: 501 1485
PasI CC'CwG_GG
Cuts at: 0 1612 1986
Size: 1612 374
PsiI TTA'TAA
Cuts at: 0 1505 1986
Size: 1505 481
PspOMI G'GGCC_C
Cuts at: 0 13 57 1986
Size: 13 44 1929
Fragments arranged by size:
1929 44 13
PstI C_TGCA'G
Cuts at: 0 198 1399 1986
Size: 198 1201 587
Fragments arranged by size:
1201 587 198
SacI G_AGCT'C
Cuts at: 0 541 1986
Size: 541 1445
SapI GCTCTTCn'nnn_
Cuts at: 0 1889 1986
Size: 1889 97
ScaI AGT'ACT
Cuts at: 0 1324 1986
Size: 1324 662
SfcI C'TryA_G
Cuts at: 0 194 581 1395 1986
Size: 194 387 814 591
Fragments arranged by size:
814 591 387 194
SmaI CCC'GGG
Cuts at: 0 56 1986
Size: 56 1930
SmlI C'TyrA_G
Cuts at: 0 271 344 1986
Size: 271 73 1642
Fragments arranged by size:
1642 271 73
SspI AAT'ATT
Cuts at: 0 1510 1986
Size: 1510 476
StuI AGG'CCT
Cuts at: 0 1447 1986
Size: 1447 539
StyI C'CwwG_G
Cuts at: 0 782 1457 1986
Size: 782 675 529
Fragments arranged by size:
782 675 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1625 1854 1986
Size: 1625 229 132
Fragments arranged by size:
1625 229 132
TatI w'GTAC_w
Cuts at: 0 1184 1322 1986
Size: 1184 138 664
Fragments arranged by size:
1184 664 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 62 763 795 1986
Size: 30 32 701 32 1191
Fragments arranged by size:
1191 701 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 203 1986
Size: 203 1783
XmaI C'CCGG_G
Cuts at: 0 54 1986
Size: 54 1932
XmnI GAAnn'nnTTC
Cuts at: 0 479 1986
Size: 479 1507
Enzymes that do cut:
AflII AflIII AleI AlwNI ApaI ApoI AvaI BamHI
BanII BbsI BclI BglII BlpI Bme1580I BmtI BplI
BpmI BpuEI BsaI BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BsrBI BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I
HindIII MscI MslI NcoI NdeI NheI NruI PasI
PsiI PspOMI PstI SacI SapI ScaI SfcI SmaI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BmgBI BmrI Bpu10I BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NotI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI XbaI XcmI
XhoI ZraI